Novel integrons and gene cassettes from a Cascadian submarine gas-hydrate-bearing core

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Journal Article
FEMS Microbiology Ecology, 2014, 87 (2)
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To determine if integrons are present in a submarine gas hydrate community, metagenomic DNA was extracted from a gas hydrate-bearing core, 150 m below the sea floor, from the Cascadian Margin. Integrons and gene cassettes were recovered by PCR from metagenomic DNA and sequenced. Thirty seven integron integrase phylotypes were identified. The phylotypes were diverse and included members with homology to integrases from Methylomonas methanica, Desulfuromonas acetoxidans, Thermodesulfatator indicus and marine uncultured bacteria. The gene cassette composition, 153 gene cassettes, was dominated by two types of encoded putative proteins. The first of these was predicted oxido/reductases, such as iron/sulfur cluster-binding proteins. A second type was alkyl transferases. Some cassette proteins showed homologies with those from methane-related archaea. These observations suggest that integrons may assist in the adaptation of microbial communities in this environment.
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