Diatom acclimation to elevated CO 2 via cAMP signalling and coordinated gene expression

Hennon, GMM; Ashworth, J; Groussman, RD; Berthiaume, C; Morales, RL; Baliga, NS; Orellana, MV; Armbrust, EV

Diatom acclimation to elevated CO 2 via cAMP signalling and coordinated gene expression

Hennon, GMM Ashworth, J

Groussman, RD Berthiaume, C Morales, RL Baliga, NS Orellana, MV Armbrust, EV

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Publication Type:: Journal Article
Citation:: Nature Climate Change, 2015, 5 (8), pp. 761 - 765
Issue Date:: 2015-01-01

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Field	Value	Language
dc.contributor.author	Hennon, GMM	en_US
dc.contributor.author	Ashworth, J https://orcid.org/0000-0003-4835-6551	en_US
dc.contributor.author	Groussman, RD	en_US
dc.contributor.author	Berthiaume, C	en_US
dc.contributor.author	Morales, RL	en_US
dc.contributor.author	Baliga, NS	en_US
dc.contributor.author	Orellana, MV	en_US
dc.contributor.author	Armbrust, EV	en_US
dc.date.issued	2015-01-01	en_US
dc.identifier.citation	Nature Climate Change, 2015, 5 (8), pp. 761 - 765	en_US
dc.identifier.issn	1758-678X	en_US
dc.identifier.uri	http://hdl.handle.net/10453/119048
dc.description.abstract	© 2015 Macmillan Publishers Limited. Diatoms are responsible for â 1/440% of marine primary productivity, fuelling the oceanic carbon cycle and contributing to natural carbon sequestration in the deep ocean. Diatoms rely on energetically expensive carbon concentrating mechanisms (CCMs) to fix carbon efficiently at modern levels of CO 2 (refs). How diatoms may respond over the short and long term to rising atmospheric CO 2 remains an open question. Here we use nitrate-limited chemostats to show that the model diatom Thalassiosira pseudonana rapidly responds to increasing CO 2 by differentially expressing gene clusters that regulate transcription and chromosome folding, and subsequently reduces transcription of photosynthesis and respiration gene clusters under steady-state elevated CO 2. These results suggest that exposure to elevated CO 2 first causes a shift in regulation, and then a metabolic rearrangement. Genes in one CO 2 -responsive cluster included CCM and photorespiration genes that share a putative cAMP-responsive cis-regulatory sequence, implying these genes are co-regulated in response to CO 2, with cAMP as an intermediate messenger. We verified cAMP-induced downregulation of CCM gene-CA3 in nutrient-replete diatom cultures by inhibiting the hydrolysis of cAMP. These results indicate an important role for cAMP in downregulating CCM and photorespiration genes under elevated CO 2 and provide insights into mechanisms of diatom acclimation in response to climate change.	en_US
dc.relation.ispartof	Nature Climate Change	en_US
dc.relation.isbasedon	10.1038/nclimate2683	en_US
dc.title	Diatom acclimation to elevated CO 2 via cAMP signalling and coordinated gene expression	en_US
dc.type	Journal Article
utslib.citation.volume	8	en_US
utslib.citation.volume	5	en_US
utslib.for	060405 Gene Expression (incl. Microarray and other genome-wide approaches)	en_US
utslib.for	050101 Ecological Impacts of Climate Change	en_US
utslib.for	0502 Environmental Science and Management	en_US
utslib.for	0401 Atmospheric Sciences	en_US
utslib.for	0406 Physical Geography and Environmental Geoscience	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	closed_access
pubs.issue	8	en_US
pubs.publication-status	Published	en_US
pubs.volume	5	en_US

Abstract:

© 2015 Macmillan Publishers Limited. Diatoms are responsible for â 1/440% of marine primary productivity, fuelling the oceanic carbon cycle and contributing to natural carbon sequestration in the deep ocean. Diatoms rely on energetically expensive carbon concentrating mechanisms (CCMs) to fix carbon efficiently at modern levels of CO 2 (refs). How diatoms may respond over the short and long term to rising atmospheric CO 2 remains an open question. Here we use nitrate-limited chemostats to show that the model diatom Thalassiosira pseudonana rapidly responds to increasing CO 2 by differentially expressing gene clusters that regulate transcription and chromosome folding, and subsequently reduces transcription of photosynthesis and respiration gene clusters under steady-state elevated CO 2. These results suggest that exposure to elevated CO 2 first causes a shift in regulation, and then a metabolic rearrangement. Genes in one CO 2 -responsive cluster included CCM and photorespiration genes that share a putative cAMP-responsive cis-regulatory sequence, implying these genes are co-regulated in response to CO 2, with cAMP as an intermediate messenger. We verified cAMP-induced downregulation of CCM gene-CA3 in nutrient-replete diatom cultures by inhibiting the hydrolysis of cAMP. These results indicate an important role for cAMP in downregulating CCM and photorespiration genes under elevated CO 2 and provide insights into mechanisms of diatom acclimation in response to climate change.

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http://hdl.handle.net/10453/119048