DdrO is an essential protein that regulates the radiation desiccation response and the apoptotic-like cell death in the radioresistant Deinococcus radiodurans bacterium

Devigne, A; Ithurbide, S; Bouthier de la Tour, C; Passot, F; Mathieu, M; Sommer, S; Servant, P

DdrO is an essential protein that regulates the radiation desiccation response and the apoptotic-like cell death in the radioresistant Deinococcus radiodurans bacterium

Devigne, A Ithurbide, S

Bouthier de la Tour, C Passot, F Mathieu, M Sommer, S Servant, P

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Publication Type:: Journal Article
Citation:: Molecular Microbiology, 2015, 96 (5), pp. 1069 - 1084
Issue Date:: 2015-06-01

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Field	Value	Language
dc.contributor.author	Devigne, A	en_US
dc.contributor.author	Ithurbide, S https://orcid.org/0000-0002-1533-8472	en_US
dc.contributor.author	Bouthier de la Tour, C	en_US
dc.contributor.author	Passot, F	en_US
dc.contributor.author	Mathieu, M	en_US
dc.contributor.author	Sommer, S	en_US
dc.contributor.author	Servant, P	en_US
dc.date.available	2015-03-02	en_US
dc.date.issued	2015-06-01	en_US
dc.identifier.citation	Molecular Microbiology, 2015, 96 (5), pp. 1069 - 1084	en_US
dc.identifier.issn	0950-382X	en_US
dc.identifier.uri	http://hdl.handle.net/10453/119189
dc.description.abstract	© 2015 John Wiley & Sons Ltd. Deinococcus radiodurans is known for its extreme radioresistance. Comparative genomics identified a radiation-desiccation response (RDR) regulon comprising genes that are highly induced after DNA damage and containing a conserved motif (RDRM) upstream of their coding region. We demonstrated that the RDRM sequence is involved in cis-regulation of the RDR gene ddrBin vivo. Using a transposon mutagenesis approach, we showed that, in addition to ddrO encoding a predicted RDR repressor and irrE encoding a positive regulator recently shown to cleave DdrO in Deinococcusdeserti, two genes encoding α-keto-glutarate dehydrogenase subunits are involved in ddrB regulation. In wild-type cells, the DdrO cell concentration decreased transiently in an IrrE-dependent manner at early times after irradiation. Using a conditional gene inactivation system, we showed that DdrO depletion enhanced expression of three RDR proteins, consistent with the hypothesis that DdrO acts as a repressor of the RDR regulon. DdrO-depleted cells loose viability and showed morphological changes evocative of an apoptotic-like response, including membrane blebbing, defects in cell division and DNA fragmentation. We propose that DNA repair and apoptotic-like death might be two responses mediated by the same regulators, IrrE and DdrO, but differently activated depending on the persistence of IrrE-dependent DdrO cleavage. Deinococcus radiodurans is known for its extreme radioresistance. We present a model for the regulation of the radiation desiccation response (RDR) regulon comprising genes highly induced after DNA damage and containing a conserved motif (RDRM) upstream of their coding region. We propose that DNA repair and apoptotic-like death might be two responses mediated by the same regulators, IrrE and DdrO, but differently activated depending on the persistence of IrrE-dependent DdrO cleavage.	en_US
dc.relation.ispartof	Molecular Microbiology	en_US
dc.relation.isbasedon	10.1111/mmi.12991	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Deinococcus	en_US
dc.subject.mesh	Dehydration	en_US
dc.subject.mesh	DNA Damage	en_US
dc.subject.mesh	Ketoglutarate Dehydrogenase Complex	en_US
dc.subject.mesh	Bacterial Proteins	en_US
dc.subject.mesh	Genomics	en_US
dc.subject.mesh	DNA Repair	en_US
dc.subject.mesh	Gene Expression Regulation, Bacterial	en_US
dc.subject.mesh	Mutagenesis	en_US
dc.subject.mesh	Amino Acid Sequence	en_US
dc.subject.mesh	Protein Structure, Tertiary	en_US
dc.subject.mesh	Open Reading Frames	en_US
dc.subject.mesh	Regulon	en_US
dc.subject.mesh	Promoter Regions, Genetic	en_US
dc.title	DdrO is an essential protein that regulates the radiation desiccation response and the apoptotic-like cell death in the radioresistant Deinococcus radiodurans bacterium	en_US
dc.type	Journal Article
utslib.citation.volume	5	en_US
utslib.citation.volume	96	en_US
utslib.for	060599 Microbiology not elsewhere classified	en_US
utslib.for	070707 Veterinary Microbiology (excl. Virology)	en_US
utslib.for	110899 Medical Microbiology not elsewhere classified	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	07 Agricultural and Veterinary Sciences	en_US
utslib.for	11 Medical and Health Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	closed_access
pubs.issue	5	en_US
pubs.publication-status	Published	en_US
pubs.volume	96	en_US

Abstract:

© 2015 John Wiley & Sons Ltd. Deinococcus radiodurans is known for its extreme radioresistance. Comparative genomics identified a radiation-desiccation response (RDR) regulon comprising genes that are highly induced after DNA damage and containing a conserved motif (RDRM) upstream of their coding region. We demonstrated that the RDRM sequence is involved in cis-regulation of the RDR gene ddrBin vivo. Using a transposon mutagenesis approach, we showed that, in addition to ddrO encoding a predicted RDR repressor and irrE encoding a positive regulator recently shown to cleave DdrO in Deinococcusdeserti, two genes encoding α-keto-glutarate dehydrogenase subunits are involved in ddrB regulation. In wild-type cells, the DdrO cell concentration decreased transiently in an IrrE-dependent manner at early times after irradiation. Using a conditional gene inactivation system, we showed that DdrO depletion enhanced expression of three RDR proteins, consistent with the hypothesis that DdrO acts as a repressor of the RDR regulon. DdrO-depleted cells loose viability and showed morphological changes evocative of an apoptotic-like response, including membrane blebbing, defects in cell division and DNA fragmentation. We propose that DNA repair and apoptotic-like death might be two responses mediated by the same regulators, IrrE and DdrO, but differently activated depending on the persistence of IrrE-dependent DdrO cleavage. Deinococcus radiodurans is known for its extreme radioresistance. We present a model for the regulation of the radiation desiccation response (RDR) regulon comprising genes highly induced after DNA damage and containing a conserved motif (RDRM) upstream of their coding region. We propose that DNA repair and apoptotic-like death might be two responses mediated by the same regulators, IrrE and DdrO, but differently activated depending on the persistence of IrrE-dependent DdrO cleavage.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/119189