Exploring the structure of biological macromolecules in solution using Quokka, the small angle neutron scattering instrument, at ANSTO

Wood, K; Jeffries, CM; Knott, RB; Sokolova, A; Jacques, DA; Duff, AP

Exploring the structure of biological macromolecules in solution using Quokka, the small angle neutron scattering instrument, at ANSTO

Wood, K Jeffries, CM Knott, RB Sokolova, A Jacques, DA

Duff, AP

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Publication Type:: Journal Article
Citation:: Nuclear Instruments and Methods in Physics Research, Section A: Accelerators, Spectrometers, Detectors and Associated Equipment, 2015, 798 pp. 44 - 51
Issue Date:: 2015-07-31

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Field	Value	Language
dc.contributor.author	Wood, K	en_US
dc.contributor.author	Jeffries, CM	en_US
dc.contributor.author	Knott, RB	en_US
dc.contributor.author	Sokolova, A	en_US
dc.contributor.author	Jacques, DA https://orcid.org/0000-0002-6426-4510	en_US
dc.contributor.author	Duff, AP	en_US
dc.date.issued	2015-07-31	en_US
dc.identifier.citation	Nuclear Instruments and Methods in Physics Research, Section A: Accelerators, Spectrometers, Detectors and Associated Equipment, 2015, 798 pp. 44 - 51	en_US
dc.identifier.issn	0168-9002	en_US
dc.identifier.uri	http://hdl.handle.net/10453/119268
dc.description.abstract	© 2015 Published by Elsevier B.V. Abstract Small angle neutron scattering (SANS) is widely used to extract structural parameters, shape and other types of information from a vast array of materials. The technique is applied to biological macromolecules and their complexes in solution to reveal information often not accessible by other techniques. SANS measurements on biomolecules present some particular challenges however, one of which is suitable instrumentation. This review details SANS experiments performed on two well-characterised globular proteins (lysozyme and glucose isomerase) using Quokka, the recently commissioned SANS instrument at the Australian Nuclear Science and Technology Organisation (ANSTO). The instrument configuration as well as data collection and reduction strategies for biological investigations are discussed and act as a general reference for structural biologists who use the instrument. Both model independent analysis of the two proteins and ab initio modelling illustrate that Quokka-SANS data can be used to successfully model the overall shapes of proteins in solution, providing a benchmark for users.	en_US
dc.relation.ispartof	Nuclear Instruments and Methods in Physics Research, Section A: Accelerators, Spectrometers, Detectors and Associated Equipment	en_US
dc.relation.isbasedon	10.1016/j.nima.2015.06.034	en_US
dc.subject.classification	Nuclear & Particles Physics	en_US
dc.title	Exploring the structure of biological macromolecules in solution using Quokka, the small angle neutron scattering instrument, at ANSTO	en_US
dc.type	Journal Article
utslib.citation.volume	798	en_US
utslib.for	0202 Atomic, Molecular, Nuclear, Particle and Plasma Physics	en_US
utslib.for	0201 Astronomical and Space Sciences	en_US
utslib.for	0299 Other Physical Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
utslib.copyright.status	closed_access
pubs.publication-status	Published	en_US
pubs.volume	798	en_US

Abstract:

© 2015 Published by Elsevier B.V. Abstract Small angle neutron scattering (SANS) is widely used to extract structural parameters, shape and other types of information from a vast array of materials. The technique is applied to biological macromolecules and their complexes in solution to reveal information often not accessible by other techniques. SANS measurements on biomolecules present some particular challenges however, one of which is suitable instrumentation. This review details SANS experiments performed on two well-characterised globular proteins (lysozyme and glucose isomerase) using Quokka, the recently commissioned SANS instrument at the Australian Nuclear Science and Technology Organisation (ANSTO). The instrument configuration as well as data collection and reduction strategies for biological investigations are discussed and act as a general reference for structural biologists who use the instrument. Both model independent analysis of the two proteins and ab initio modelling illustrate that Quokka-SANS data can be used to successfully model the overall shapes of proteins in solution, providing a benchmark for users.

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http://hdl.handle.net/10453/119268