Dynamic tubulation of mitochondria drives mitochondrial network formation

Wang, C; Du, W; Su, QP; Zhu, M; Feng, P; Li, Y; Zhou, Y; Mi, N; Zhu, Y; Jiang, D; Zhang, S; Zhang, Z; Sun, Y; Yu, L

Dynamic tubulation of mitochondria drives mitochondrial network formation

Wang, C Du, W Su, QP

Zhu, M Feng, P Li, Y Zhou, Y Mi, N Zhu, Y Jiang, D Zhang, S Zhang, Z Sun, Y Yu, L

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Publication Type:: Journal Article
Citation:: Cell Research, 2015, 25 (10), pp. 1108 - 1120
Issue Date:: 2015-01-01

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Field	Value	Language
dc.contributor.author	Wang, C	en_US
dc.contributor.author	Du, W	en_US
dc.contributor.author	Su, QP https://orcid.org/0000-0001-7364-3945	en_US
dc.contributor.author	Zhu, M	en_US
dc.contributor.author	Feng, P	en_US
dc.contributor.author	Li, Y	en_US
dc.contributor.author	Zhou, Y	en_US
dc.contributor.author	Mi, N	en_US
dc.contributor.author	Zhu, Y	en_US
dc.contributor.author	Jiang, D	en_US
dc.contributor.author	Zhang, S	en_US
dc.contributor.author	Zhang, Z	en_US
dc.contributor.author	Sun, Y	en_US
dc.contributor.author	Yu, L	en_US
dc.date.available	2015-06-05	en_US
dc.date.issued	2015-01-01	en_US
dc.identifier.citation	Cell Research, 2015, 25 (10), pp. 1108 - 1120	en_US
dc.identifier.issn	1001-0602	en_US
dc.identifier.uri	http://hdl.handle.net/10453/119448
dc.description.abstract	© 2015 IBCB, SIBS, CAS. Mitochondria form networks. Formation of mitochondrial networks is important for maintaining mitochondrial DNA integrity and interchanging mitochondrial material, whereas disruption of the mitochondrial network affects mitochondrial functions. According to the current view, mitochondrial networks are formed by fusion of individual mitochondria. Here, we report a new mechanism for formation of mitochondrial networks through KIF5B-mediated dynamic tubulation of mitochondria. We found that KIF5B pulls thin, highly dynamic tubules out of mitochondria. Fusion of these dynamic tubules, which is mediated by mitofusins, gives rise to the mitochondrial network. We further demonstrated that dynamic tubulation and fusion is sufficient for mitochondrial network formation, by reconstituting mitochondrial networks in vitro using purified fusion-competent mitochondria, recombinant KIF5B, and polymerized microtubules. Interestingly, KIF5B only controls network formation in the peripheral zone of the cell, indicating that the mitochondrial network is divided into subzones, which may be constructed by different mechanisms. Our data not only uncover an essential mechanism for mitochondrial network formation, but also reveal that different parts of the mitochondrial network are formed by different mechanisms.	en_US
dc.relation.ispartof	Cell Research	en_US
dc.relation.isbasedon	10.1038/cr.2015.89	en_US
dc.subject.classification	Developmental Biology	en_US
dc.subject.mesh	Cell Line	en_US
dc.subject.mesh	Mitochondria	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Rats	en_US
dc.subject.mesh	Kinesin	en_US
dc.subject.mesh	Membrane Proteins	en_US
dc.subject.mesh	Mitochondrial Proteins	en_US
dc.subject.mesh	Mitochondrial Dynamics	en_US
dc.title	Dynamic tubulation of mitochondria drives mitochondrial network formation	en_US
dc.type	Journal Article
utslib.citation.volume	10	en_US
utslib.citation.volume	25	en_US
utslib.for	0601 Biochemistry and Cell Biology	en_US
utslib.for	1103 Clinical Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
utslib.copyright.status	open_access
pubs.issue	10	en_US
pubs.publication-status	Published	en_US
pubs.volume	25	en_US

Abstract:

© 2015 IBCB, SIBS, CAS. Mitochondria form networks. Formation of mitochondrial networks is important for maintaining mitochondrial DNA integrity and interchanging mitochondrial material, whereas disruption of the mitochondrial network affects mitochondrial functions. According to the current view, mitochondrial networks are formed by fusion of individual mitochondria. Here, we report a new mechanism for formation of mitochondrial networks through KIF5B-mediated dynamic tubulation of mitochondria. We found that KIF5B pulls thin, highly dynamic tubules out of mitochondria. Fusion of these dynamic tubules, which is mediated by mitofusins, gives rise to the mitochondrial network. We further demonstrated that dynamic tubulation and fusion is sufficient for mitochondrial network formation, by reconstituting mitochondrial networks in vitro using purified fusion-competent mitochondria, recombinant KIF5B, and polymerized microtubules. Interestingly, KIF5B only controls network formation in the peripheral zone of the cell, indicating that the mitochondrial network is divided into subzones, which may be constructed by different mechanisms. Our data not only uncover an essential mechanism for mitochondrial network formation, but also reveal that different parts of the mitochondrial network are formed by different mechanisms.

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http://hdl.handle.net/10453/119448