A modified method for purification of biological samples collected on FTA cards for STR analysis

Barash, M; Shpitzen, M; Gafny, R; Zamir, A

A modified method for purification of biological samples collected on FTA cards for STR analysis

Barash, M

Shpitzen, M Gafny, R Zamir, A

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Publication Type:: Journal Article
Citation:: Journal of Forensic Identification, 2015, 65 (4), pp. 662 - 670
Issue Date:: 2015-01-01

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Field	Value	Language
dc.contributor.author	Barash, M https://orcid.org/0000-0002-5445-6316	en_US
dc.contributor.author	Shpitzen, M	en_US
dc.contributor.author	Gafny, R	en_US
dc.contributor.author	Zamir, A	en_US
dc.date.issued	2015-01-01	en_US
dc.identifier.citation	Journal of Forensic Identification, 2015, 65 (4), pp. 662 - 670	en_US
dc.identifier.issn	0895-173X	en_US
dc.identifier.uri	http://hdl.handle.net/10453/119493
dc.description.abstract	FTA cards are regarded as a method of choice for the preservation and storage of blood and saliva prior to genetic testing. In this study, we compared the recommended protocol of DNA purification and amplification from FTA cards to a method we developed in the laboratory. Our simplified method was tailored for amplification using the AmpFLSTR SGM Plus kit. The modified method proved to decrease artifacts in DNA profiles and was more time- and cost-effective than the manufacturer's protocol.	en_US
dc.relation.ispartof	Journal of Forensic Identification	en_US
dc.subject.classification	Legal & Forensic Medicine	en_US
dc.title	A modified method for purification of biological samples collected on FTA cards for STR analysis	en_US
dc.type	Journal Article
utslib.citation.volume	4	en_US
utslib.citation.volume	65	en_US
utslib.for	0399 Other Chemical Sciences	en_US
utslib.for	0699 Other Biological Sciences	en_US
utslib.for	1801 Law	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
utslib.copyright.status	closed_access
pubs.issue	4	en_US
pubs.publication-status	Published	en_US
pubs.volume	65	en_US

Abstract:

FTA cards are regarded as a method of choice for the preservation and storage of blood and saliva prior to genetic testing. In this study, we compared the recommended protocol of DNA purification and amplification from FTA cards to a method we developed in the laboratory. Our simplified method was tailored for amplification using the AmpFLSTR SGM Plus kit. The modified method proved to decrease artifacts in DNA profiles and was more time- and cost-effective than the manufacturer's protocol.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/119493