A modified method for purification of biological samples collected on FTA cards for STR analysis
- Publication Type:
- Journal Article
- Journal of Forensic Identification, 2015, 65 (4), pp. 662 - 670
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FTA cards are regarded as a method of choice for the preservation and storage of blood and saliva prior to genetic testing. In this study, we compared the recommended protocol of DNA purification and amplification from FTA cards to a method we developed in the laboratory. Our simplified method was tailored for amplification using the AmpFLSTR SGM Plus kit. The modified method proved to decrease artifacts in DNA profiles and was more time- and cost-effective than the manufacturer's protocol.
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