A Liver Capsular Network of Monocyte-Derived Macrophages Restricts Hepatic Dissemination of Intraperitoneal Bacteria by Neutrophil Recruitment

Sierro, F; Evrard, M; Rizzetto, S; Melino, M; Mitchell, AJ; Florido, M; Beattie, L; Walters, SB; Tay, SS; Lu, B; Holz, LE; Roediger, B; Wong, YC; Warren, A; Ritchie, W; McGuffog, C; Weninger, W; Le Couteur, DG; Ginhoux, F; Britton, WJ; Heath, WR; Saunders, BM; McCaughan, GW; Luciani, F; MacDonald, KPA; Ng, LG; Bowen, DG; Bertolino, P

A Liver Capsular Network of Monocyte-Derived Macrophages Restricts Hepatic Dissemination of Intraperitoneal Bacteria by Neutrophil Recruitment

Sierro, F Evrard, M Rizzetto, S Melino, M Mitchell, AJ Florido, M Beattie, L Walters, SB Tay, SS Lu, B Holz, LE Roediger, B Wong, YC Warren, A Ritchie, W McGuffog, C Weninger, W Le Couteur, DG Ginhoux, F Britton, WJ Heath, WR Saunders, BM

McCaughan, GW Luciani, F MacDonald, KPA Ng, LG Bowen, DG Bertolino, P

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Publication Type:: Journal Article
Citation:: Immunity, 2017, 47 (2), pp. 374 - 388.e6
Issue Date:: 2017-08-15

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Field	Value	Language
dc.contributor.author	Sierro, F	en_US
dc.contributor.author	Evrard, M	en_US
dc.contributor.author	Rizzetto, S	en_US
dc.contributor.author	Melino, M	en_US
dc.contributor.author	Mitchell, AJ	en_US
dc.contributor.author	Florido, M	en_US
dc.contributor.author	Beattie, L	en_US
dc.contributor.author	Walters, SB	en_US
dc.contributor.author	Tay, SS	en_US
dc.contributor.author	Lu, B	en_US
dc.contributor.author	Holz, LE	en_US
dc.contributor.author	Roediger, B	en_US
dc.contributor.author	Wong, YC	en_US
dc.contributor.author	Warren, A	en_US
dc.contributor.author	Ritchie, W	en_US
dc.contributor.author	McGuffog, C	en_US
dc.contributor.author	Weninger, W	en_US
dc.contributor.author	Le Couteur, DG	en_US
dc.contributor.author	Ginhoux, F	en_US
dc.contributor.author	Britton, WJ	en_US
dc.contributor.author	Heath, WR	en_US
dc.contributor.author	Saunders, BM https://orcid.org/0000-0003-0540-4445	en_US
dc.contributor.author	McCaughan, GW	en_US
dc.contributor.author	Luciani, F	en_US
dc.contributor.author	MacDonald, KPA	en_US
dc.contributor.author	Ng, LG	en_US
dc.contributor.author	Bowen, DG	en_US
dc.contributor.author	Bertolino, P	en_US
dc.date.available	2017-07-23	en_US
dc.date.issued	2017-08-15	en_US
dc.identifier.citation	Immunity, 2017, 47 (2), pp. 374 - 388.e6	en_US
dc.identifier.issn	1074-7613	en_US
dc.identifier.uri	http://hdl.handle.net/10453/119629
dc.description.abstract	© 2017 Elsevier Inc. The liver is positioned at the interface between two routes traversed by pathogens in disseminating infection. Whereas blood-borne pathogens are efficiently cleared in hepatic sinusoids by Kupffer cells (KCs), it is unknown how the liver prevents dissemination of peritoneal pathogens accessing its outer membrane. We report here that the hepatic capsule harbors a contiguous cellular network of liver-resident macrophages phenotypically distinct from KCs. These liver capsular macrophages (LCMs) were replenished in the steady state from blood monocytes, unlike KCs that are embryonically derived and self-renewing. LCM numbers increased after weaning in a microbiota-dependent process. LCMs sensed peritoneal bacteria and promoted neutrophil recruitment to the capsule, and their specific ablation resulted in decreased neutrophil recruitment and increased intrahepatic bacterial burden. Thus, the liver contains two separate and non-overlapping niches occupied by distinct resident macrophage populations mediating immunosurveillance at these two pathogen entry points to the liver.	en_US
dc.relation.ispartof	Immunity	en_US
dc.relation.isbasedon	10.1016/j.immuni.2017.07.018	en_US
dc.subject.classification	Immunology	en_US
dc.subject.mesh	Peritoneum	en_US
dc.subject.mesh	Liver	en_US
dc.subject.mesh	Neutrophils	en_US
dc.subject.mesh	Monocytes	en_US
dc.subject.mesh	Macrophages	en_US
dc.subject.mesh	Kupffer Cells	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Mice, Inbred C57BL	en_US
dc.subject.mesh	Mice, Knockout	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Mice	en_US
dc.subject.mesh	Listeria monocytogenes	en_US
dc.subject.mesh	Cell Communication	en_US
dc.subject.mesh	Neutrophil Infiltration	en_US
dc.subject.mesh	Host-Pathogen Interactions	en_US
dc.subject.mesh	Immunity, Innate	en_US
dc.subject.mesh	Listeriosis	en_US
dc.subject.mesh	Cell Self Renewal	en_US
dc.title	A Liver Capsular Network of Monocyte-Derived Macrophages Restricts Hepatic Dissemination of Intraperitoneal Bacteria by Neutrophil Recruitment	en_US
dc.type	Journal Article
utslib.citation.volume	2	en_US
utslib.citation.volume	47	en_US
utslib.for	1107 Immunology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access
pubs.issue	2	en_US
pubs.publication-status	Published	en_US
pubs.volume	47	en_US

Abstract:

© 2017 Elsevier Inc. The liver is positioned at the interface between two routes traversed by pathogens in disseminating infection. Whereas blood-borne pathogens are efficiently cleared in hepatic sinusoids by Kupffer cells (KCs), it is unknown how the liver prevents dissemination of peritoneal pathogens accessing its outer membrane. We report here that the hepatic capsule harbors a contiguous cellular network of liver-resident macrophages phenotypically distinct from KCs. These liver capsular macrophages (LCMs) were replenished in the steady state from blood monocytes, unlike KCs that are embryonically derived and self-renewing. LCM numbers increased after weaning in a microbiota-dependent process. LCMs sensed peritoneal bacteria and promoted neutrophil recruitment to the capsule, and their specific ablation resulted in decreased neutrophil recruitment and increased intrahepatic bacterial burden. Thus, the liver contains two separate and non-overlapping niches occupied by distinct resident macrophage populations mediating immunosurveillance at these two pathogen entry points to the liver.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/119629