Plasmonic Ag/SiO<inf>2</inf>composite nanoparticles doped with europium chelate and their metal enhanced fluorescence

Publisher:
SPIE
Publication Type:
Conference Proceeding
Citation:
Progress in Biomedical Optics and Imaging - Proceedings of SPIE, 2011, 7909
Issue Date:
2011-03-21
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We report silver nanostructure-enhanced fluorescence of a europium (Eu) chelate, BHHCT-Eu-DPBT, which was covalently bound in Ag/SiO 2 nanocomposites. This design enhances the europium signal intensity by more than one order of magnitude, and accelerates the decay time from 0.3 ms down to 60 microseconds, at low excitation conditions. These nanocomposites were bright enough to be observed in time-gated fluorescence microscopy under 365 nm LED excitation. The increased brightness and reduced lifetime of such fluorescent core-shell nanocomposites will enhance their applicability for ultrasensitive bioassays and bioimaging, especially with time-gating. © 2011 SPIE.
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