In situ metabolomic- and transcriptomic-profiling of the host-associated cyanobacteria Prochloron and Acaryochloris marina

Behrendt, L; Raina, JB; Lutz, A; Kot, W; Albertsen, M; Halkjær-Nielsen, P; Sørensen, SJ; Larkum, AW; Kühl, M

In situ metabolomic- and transcriptomic-profiling of the host-associated cyanobacteria Prochloron and Acaryochloris marina

Behrendt, L Raina, JB

Lutz, A Kot, W Albertsen, M Halkjær-Nielsen, P Sørensen, SJ Larkum, AW

Kühl, M

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Publication Type:: Journal Article
Citation:: ISME Journal, 2018, 12 (2), pp. 556 - 567
Issue Date:: 2018-02-01

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Field	Value	Language
dc.contributor.author	Behrendt, L	en_US
dc.contributor.author	Raina, JB https://orcid.org/0000-0002-7508-0004	en_US
dc.contributor.author	Lutz, A	en_US
dc.contributor.author	Kot, W	en_US
dc.contributor.author	Albertsen, M	en_US
dc.contributor.author	Halkjær-Nielsen, P	en_US
dc.contributor.author	Sørensen, SJ	en_US
dc.contributor.author	Larkum, AW https://orcid.org/0000-0002-0420-134X	en_US
dc.contributor.author	Kühl, M https://orcid.org/0000-0002-1792-4790	en_US
dc.date.available	2020-05-25T19:25:43Z
dc.date.issued	2018-02-01	en_US
dc.identifier.citation	ISME Journal, 2018, 12 (2), pp. 556 - 567	en_US
dc.identifier.issn	1751-7362	en_US
dc.identifier.uri	http://hdl.handle.net/10453/120737
dc.description.abstract	© 2018 International Society for Microbial Ecology All rights reserved 1751-7362/18. The tropical ascidian Lissoclinum patella hosts two enigmatic cyanobacteria: (1) the photoendosymbiont Prochloron spp., a producer of valuable bioactive compounds and (2) the chlorophyll-d containing Acaryochloris spp., residing in the near-infrared enriched underside of the animal. Despite numerous efforts, Prochloron remains uncultivable, restricting the investigation of its biochemical potential to cultivation-independent techniques. Likewise, in both cyanobacteria, universally important parameters on light-niche adaptation and in situ photosynthetic regulation are unknown. Here we used genome sequencing, transcriptomics and metabolomics to investigate the symbiotic linkage between host and photoendosymbiont and simultaneously probed the transcriptional response of Acaryochloris in situ. During high light, both cyanobacteria downregulate CO 2 fixing pathways, likely a result of O 2 photorespiration on the functioning of RuBisCO, and employ a variety of stress-quenching mechanisms, even under less stressful far-red light (Acaryochloris). Metabolomics reveals a distinct biochemical modulation between Prochloron and L. patella, including noon/midnight-dependent signatures of amino acids, nitrogenous waste products and primary photosynthates. Surprisingly, Prochloron constitutively expressed genes coding for patellamides, that is, cyclic peptides of great pharmaceutical value, with yet unknown ecological significance. Together these findings shed further light on far-red-driven photosynthesis in natural consortia, the interplay of Prochloron and its ascidian partner in a model chordate photosymbiosis and the uncultivability of Prochloron.	en_US
dc.relation.ispartof	ISME Journal	en_US
dc.relation.isbasedon	10.1038/ismej.2017.192	en_US
dc.subject.classification	Microbiology	en_US
dc.title	In situ metabolomic- and transcriptomic-profiling of the host-associated cyanobacteria Prochloron and Acaryochloris marina	en_US
dc.type	Journal Article
utslib.citation.volume	2	en_US
utslib.citation.volume	12	en_US
utslib.for	0601 Biochemistry and Cell Biology	en_US
utslib.for	1002 Environmental Biotechnology	en_US
utslib.for	0501 Ecological Applications	en_US
utslib.for	05 Environmental Sciences	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	10 Technology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	open_access
pubs.issue	2	en_US
pubs.publication-status	Published	en_US
pubs.volume	12	en_US

Abstract:

© 2018 International Society for Microbial Ecology All rights reserved 1751-7362/18. The tropical ascidian Lissoclinum patella hosts two enigmatic cyanobacteria: (1) the photoendosymbiont Prochloron spp., a producer of valuable bioactive compounds and (2) the chlorophyll-d containing Acaryochloris spp., residing in the near-infrared enriched underside of the animal. Despite numerous efforts, Prochloron remains uncultivable, restricting the investigation of its biochemical potential to cultivation-independent techniques. Likewise, in both cyanobacteria, universally important parameters on light-niche adaptation and in situ photosynthetic regulation are unknown. Here we used genome sequencing, transcriptomics and metabolomics to investigate the symbiotic linkage between host and photoendosymbiont and simultaneously probed the transcriptional response of Acaryochloris in situ. During high light, both cyanobacteria downregulate CO 2 fixing pathways, likely a result of O 2 photorespiration on the functioning of RuBisCO, and employ a variety of stress-quenching mechanisms, even under less stressful far-red light (Acaryochloris). Metabolomics reveals a distinct biochemical modulation between Prochloron and L. patella, including noon/midnight-dependent signatures of amino acids, nitrogenous waste products and primary photosynthates. Surprisingly, Prochloron constitutively expressed genes coding for patellamides, that is, cyclic peptides of great pharmaceutical value, with yet unknown ecological significance. Together these findings shed further light on far-red-driven photosynthesis in natural consortia, the interplay of Prochloron and its ascidian partner in a model chordate photosymbiosis and the uncultivability of Prochloron.

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http://hdl.handle.net/10453/120737