The rapid in vivo evolution of Pseudomonas aeruginosa in ventilator-associated pneumonia patients leads to attenuated virulence
Wang, K
Chen, YQ
Salido, MM
Kohli, GS
Kong, JL
Liang, HJ
Yao, ZT
Xie, YT
Wu, HY
Cai, SQ
Drautz-Moses, DI
Darling, AE
Schuster, SC
Yang, L
Ding, Y
- Publication Type:
- Journal Article
- Citation:
- Open Biology, 2017, 7 (9)
- Issue Date:
- 2017-01-01
Closed Access
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The rapid in vivo evolution of Pseudomonas aeruginosa in ventilator-associated pneumonia patients leads to attenuated virulence.pdf | Accepted Manuscript Version | 1.24 MB | Adobe PDF |
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Full metadata record
Field | Value | Language |
---|---|---|
dc.contributor.author | Wang, K | en_US |
dc.contributor.author | Chen, YQ | en_US |
dc.contributor.author | Salido, MM | en_US |
dc.contributor.author |
Kohli, GS https://orcid.org/0000-0002-4578-2355 |
en_US |
dc.contributor.author | Kong, JL | en_US |
dc.contributor.author | Liang, HJ | en_US |
dc.contributor.author | Yao, ZT | en_US |
dc.contributor.author | Xie, YT | en_US |
dc.contributor.author | Wu, HY | en_US |
dc.contributor.author | Cai, SQ | en_US |
dc.contributor.author | Drautz-Moses, DI | en_US |
dc.contributor.author |
Darling, AE https://orcid.org/0000-0003-2397-7925 |
en_US |
dc.contributor.author | Schuster, SC | en_US |
dc.contributor.author | Yang, L | en_US |
dc.contributor.author | Ding, Y | en_US |
dc.date.available | 2017-07-26 | en_US |
dc.date.issued | 2017-01-01 | en_US |
dc.identifier.citation | Open Biology, 2017, 7 (9) | en_US |
dc.identifier.uri | http://hdl.handle.net/10453/121938 | |
dc.description.abstract | © 2017 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited. Pseudomonas aeruginosa is an opportunistic pathogen that causes severe airway infections in humans. These infections are usually difficult to treat and associated with high mortality rates. While colonizing the human airways, P. aeruginosa could accumulate genetic mutations that often lead to its better adaptability to the host environment. Understanding these evolutionary traits may provide important clues for the development of effective therapies to treat P. aeruginosa infections. In this study, 25 P. aeruginosa isolates were longitudinally sampled from the airways of four ventilator-associated pneumonia (VAP) patients. Pacbio and Illumina sequencing were used to analyse the in vivo evolutionary trajectories of these isolates. Our analysis showed that positive selection dominantly shaped P. aeruginosa genomes during VAP infections and led to three convergent evolution events, including loss-of-function mutations of lasR and mpl, and a pyoverdine-deficient phenotype. Specifically, lasR encodes one of the major transcriptional regulators in quorum sensing, whereas mpl encodes an enzyme responsible for recycling cell wall peptidoglycan. We also found that P. aeruginosa isolated at late stages of VAP infections produce less elastase and are less virulent in vivo than their earlier isolated counterparts, suggesting the short-term in vivo evolution of P. aeruginosa leads to attenuated virulence. | en_US |
dc.relation.ispartof | Open Biology | en_US |
dc.relation.isbasedon | 10.1098/rsob.170029 | en_US |
dc.subject.mesh | Cell Wall | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Pseudomonas aeruginosa | en_US |
dc.subject.mesh | Pseudomonas Infections | en_US |
dc.subject.mesh | Metalloendopeptidases | en_US |
dc.subject.mesh | Pancreatic Elastase | en_US |
dc.subject.mesh | Oligopeptides | en_US |
dc.subject.mesh | Bacterial Proteins | en_US |
dc.subject.mesh | Trans-Activators | en_US |
dc.subject.mesh | Siderophores | en_US |
dc.subject.mesh | Anti-Bacterial Agents | en_US |
dc.subject.mesh | Microbial Sensitivity Tests | en_US |
dc.subject.mesh | Evolution, Molecular | en_US |
dc.subject.mesh | Phylogeny | en_US |
dc.subject.mesh | Drug Resistance, Multiple, Bacterial | en_US |
dc.subject.mesh | Virulence | en_US |
dc.subject.mesh | Gene Expression Regulation, Bacterial | en_US |
dc.subject.mesh | Mutation | en_US |
dc.subject.mesh | Genome, Bacterial | en_US |
dc.subject.mesh | Pneumonia, Ventilator-Associated | en_US |
dc.subject.mesh | Quorum Sensing | en_US |
dc.title | The rapid in vivo evolution of Pseudomonas aeruginosa in ventilator-associated pneumonia patients leads to attenuated virulence | en_US |
dc.type | Journal Article | |
utslib.citation.volume | 9 | en_US |
utslib.citation.volume | 7 | en_US |
utslib.for | 0306 Physical Chemistry (incl. Structural) | en_US |
utslib.for | 0601 Biochemistry and Cell Biology | en_US |
utslib.for | 0605 Microbiology | en_US |
utslib.for | 1107 Immunology | en_US |
pubs.embargo.period | Not known | en_US |
pubs.organisational-group | /University of Technology Sydney | |
pubs.organisational-group | /University of Technology Sydney/DVC (International) | |
pubs.organisational-group | /University of Technology Sydney/DVC (International)/Australia-China Relations Institute | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Science | |
pubs.organisational-group | /University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation | |
utslib.copyright.status | closed_access | |
pubs.issue | 9 | en_US |
pubs.publication-status | Published | en_US |
pubs.volume | 7 | en_US |
Abstract:
© 2017 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited. Pseudomonas aeruginosa is an opportunistic pathogen that causes severe airway infections in humans. These infections are usually difficult to treat and associated with high mortality rates. While colonizing the human airways, P. aeruginosa could accumulate genetic mutations that often lead to its better adaptability to the host environment. Understanding these evolutionary traits may provide important clues for the development of effective therapies to treat P. aeruginosa infections. In this study, 25 P. aeruginosa isolates were longitudinally sampled from the airways of four ventilator-associated pneumonia (VAP) patients. Pacbio and Illumina sequencing were used to analyse the in vivo evolutionary trajectories of these isolates. Our analysis showed that positive selection dominantly shaped P. aeruginosa genomes during VAP infections and led to three convergent evolution events, including loss-of-function mutations of lasR and mpl, and a pyoverdine-deficient phenotype. Specifically, lasR encodes one of the major transcriptional regulators in quorum sensing, whereas mpl encodes an enzyme responsible for recycling cell wall peptidoglycan. We also found that P. aeruginosa isolated at late stages of VAP infections produce less elastase and are less virulent in vivo than their earlier isolated counterparts, suggesting the short-term in vivo evolution of P. aeruginosa leads to attenuated virulence.
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