Antimicrobial activity of T4 bacteriophage conjugated indium tin oxide surfaces

Liana, AE; Marquis, CP; Gunawan, C; Justin Gooding, J; Amal, R

Antimicrobial activity of T4 bacteriophage conjugated indium tin oxide surfaces

Liana, AE Marquis, CP Gunawan, C

Justin Gooding, J Amal, R

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Publication Type:: Journal Article
Citation:: Journal of Colloid and Interface Science, 2018, 514 pp. 227 - 233
Issue Date:: 2018-03-15

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Field	Value	Language
dc.contributor.author	Liana, AE	en_US
dc.contributor.author	Marquis, CP	en_US
dc.contributor.author	Gunawan, C https://orcid.org/0000-0002-2957-1347	en_US
dc.contributor.author	Justin Gooding, J	en_US
dc.contributor.author	Amal, R	en_US
dc.date.available	2017-12-11	en_US
dc.date.issued	2018-03-15	en_US
dc.identifier.citation	Journal of Colloid and Interface Science, 2018, 514 pp. 227 - 233	en_US
dc.identifier.issn	0021-9797	en_US
dc.identifier.uri	http://hdl.handle.net/10453/121983
dc.description.abstract	© 2017 Elsevier Inc. We report the antimicrobial activity of bare and surface functionalized indium tin oxide (ITO) conjugated with T4 bacteriophage towards E. coli. A ∼ 103-fold reduction (99.9%) in the bacterial concentration was achieved within 2 h exposure of E. coli to the bare as well as the amine, carboxylic and methyl functionalized ITO/T4 surfaces. Despite the known differences in bacteriophage loading of these ITO/T4 systems, the almost identical extent of antimicrobial activity of all of the ITO/T4 systems resulted from the release of a comparable amount of infective T4 from the systems. As anticipated, a single dose of immobilized bacteriophage was sufficient to eliminate further surge of bacterial population. Upon the 2 h eradication of the ‘1st batch’ of E. coli population, all of the ITO/T4 systems, each system with 102-fold more suspended bacteriophage (due to propagation of the phage at the expense of the ‘1st batch’ E. coli death), reduced the ‘2nd batch’ of E. coli concentration by ∼104-fold in just 30 min, suggesting the potential of immobilized bacteriophage systems as solution to the issues of antimicrobial agent depletion. All of the ITO/T4 systems maintained their antimicrobial activity in the presence of model food components. The antimicrobial activity was however, affected by pH; at pH 5 whereby the bacteria's growth was physiologically inhibited, generally no reduction in E. coli concentration was detected. The present work provides an understanding of the mode of antimicrobial activity exhibited by an immobilized bacteriophage based substrate and demonstrates efficacy in the presence of food components.	en_US
dc.relation.ispartof	Journal of Colloid and Interface Science	en_US
dc.relation.isbasedon	10.1016/j.jcis.2017.12.029	en_US
dc.subject.classification	Chemical Physics	en_US
dc.subject.mesh	Escherichia coli	en_US
dc.subject.mesh	Bacteriophage T4	en_US
dc.subject.mesh	Tin Compounds	en_US
dc.subject.mesh	Anti-Bacterial Agents	en_US
dc.subject.mesh	Microbial Sensitivity Tests	en_US
dc.subject.mesh	Surface Properties	en_US
dc.title	Antimicrobial activity of T4 bacteriophage conjugated indium tin oxide surfaces	en_US
dc.type	Journal Article
utslib.citation.volume	514	en_US
utslib.for	0305 Organic Chemistry	en_US
utslib.for	0904 Chemical Engineering	en_US
utslib.for	0202 Atomic, Molecular, Nuclear, Particle and Plasma Physics	en_US
utslib.for	02 Physical Sciences	en_US
utslib.for	03 Chemical Sciences	en_US
utslib.for	09 Engineering	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	closed_access
pubs.publication-status	Published	en_US
pubs.volume	514	en_US

Abstract:

© 2017 Elsevier Inc. We report the antimicrobial activity of bare and surface functionalized indium tin oxide (ITO) conjugated with T4 bacteriophage towards E. coli. A ∼ 103-fold reduction (99.9%) in the bacterial concentration was achieved within 2 h exposure of E. coli to the bare as well as the amine, carboxylic and methyl functionalized ITO/T4 surfaces. Despite the known differences in bacteriophage loading of these ITO/T4 systems, the almost identical extent of antimicrobial activity of all of the ITO/T4 systems resulted from the release of a comparable amount of infective T4 from the systems. As anticipated, a single dose of immobilized bacteriophage was sufficient to eliminate further surge of bacterial population. Upon the 2 h eradication of the ‘1st batch’ of E. coli population, all of the ITO/T4 systems, each system with 102-fold more suspended bacteriophage (due to propagation of the phage at the expense of the ‘1st batch’ E. coli death), reduced the ‘2nd batch’ of E. coli concentration by ∼104-fold in just 30 min, suggesting the potential of immobilized bacteriophage systems as solution to the issues of antimicrobial agent depletion. All of the ITO/T4 systems maintained their antimicrobial activity in the presence of model food components. The antimicrobial activity was however, affected by pH; at pH 5 whereby the bacteria's growth was physiologically inhibited, generally no reduction in E. coli concentration was detected. The present work provides an understanding of the mode of antimicrobial activity exhibited by an immobilized bacteriophage based substrate and demonstrates efficacy in the presence of food components.

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http://hdl.handle.net/10453/121983