Elucidation of markers for monitoring morphine and its analogs in urine adulterated with pyridinium chlorochromate

Publication Type:
Journal Article
Citation:
Bioanalysis, 2015, 7 (18), pp. 2283 - 2295
Issue Date:
2015-01-01
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© 2015 Future Science Ltd. Aim: Currently, procedures that identify the drugs 'destroyed' in adulterated urine specimens are very limited. This study aimed to determine the effect of pyridinium chlorochromate (PCC) on routine opiate assays and identify reaction products formed. Results/methodology: Opiate-positive urines adulterated with PCC (20 and 100 mM) were analyzed using CEDIA® immunoassay and GC-MS. Urine and water samples spiked with 6-monoacetylmorphine, morphine and its glucuronides (10 μg/ml) and PCC (0.02-100 mM) were monitored with LC-MS, and the products characterized. Conclusion: PCC significantly decreased the abundance of morphine, codeine and IS. Adulterated water and urine samples containing 6-monoacetylmorphine, morphine and morphine-3-glucuronide yielded morphinone-3-glucuronide, 7,14-dihydroxy-6-monoacetylmorphine, 7,8-diketo-6-monoacetylmorphine and 7,8-diketo-morphine (tentative assignment). Reaction pathways may be different in the two matrices.
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