DSYB catalyses the key step of dimethylsulfoniopropionate biosynthesis in many phytoplankton

Curson, ARJ; Williams, BT; Pinchbeck, BJ; Sims, LP; Martínez, AB; Rivera, PPL; Kumaresan, D; Mercadé, E; Spurgin, LG; Carrión, O; Moxon, S; Cattolico, RA; Kuzhiumparambil, U; Guagliardo, P; Clode, PL; Raina, JB; Todd, JD

DSYB catalyses the key step of dimethylsulfoniopropionate biosynthesis in many phytoplankton

Curson, ARJ Williams, BT Pinchbeck, BJ Sims, LP Martínez, AB Rivera, PPL Kumaresan, D Mercadé, E Spurgin, LG Carrión, O Moxon, S Cattolico, RA Kuzhiumparambil, U

Guagliardo, P Clode, PL Raina, JB

Todd, JD

Permalink

Publication Type:: Journal Article
Citation:: Nature Microbiology, 2018, 3 (4), pp. 430 - 439
Issue Date:: 2018-04-01

Open Access

Copyright Clearance Process

Recently Added
In Progress
Open Access

This item is open access.

Adobe PDF

Download Accepted Manuscript VersionAdobe PDF (2.98 MB)

View on publisher's site

View statistics

Full metadata record

Field	Value	Language
dc.contributor.author	Curson, ARJ	en_US
dc.contributor.author	Williams, BT	en_US
dc.contributor.author	Pinchbeck, BJ	en_US
dc.contributor.author	Sims, LP	en_US
dc.contributor.author	Martínez, AB	en_US
dc.contributor.author	Rivera, PPL	en_US
dc.contributor.author	Kumaresan, D	en_US
dc.contributor.author	Mercadé, E	en_US
dc.contributor.author	Spurgin, LG	en_US
dc.contributor.author	Carrión, O	en_US
dc.contributor.author	Moxon, S	en_US
dc.contributor.author	Cattolico, RA	en_US
dc.contributor.author	Kuzhiumparambil, U https://orcid.org/0000-0003-0582-6779	en_US
dc.contributor.author	Guagliardo, P	en_US
dc.contributor.author	Clode, PL	en_US
dc.contributor.author	Raina, JB https://orcid.org/0000-0002-7508-0004	en_US
dc.contributor.author	Todd, JD	en_US
dc.date.available	2020-05-25T19:04:28Z
dc.date.issued	2018-04-01	en_US
dc.identifier.citation	Nature Microbiology, 2018, 3 (4), pp. 430 - 439	en_US
dc.identifier.uri	http://hdl.handle.net/10453/122416
dc.description.abstract	© 2018 The Author(s). Dimethylsulfoniopropionate (DMSP) is a globally important organosulfur molecule and the major precursor for dimethyl sulfide. These compounds are important info-chemicals, key nutrients for marine microorganisms, and are involved in global sulfur cycling, atmospheric chemistry and cloud formation1-3. DMSP production was thought to be confined to eukaryotes, but heterotrophic bacteria can also produce DMSP through the pathway used by most phytoplankton4, and the DsyB enzyme catalysing the key step of this pathway in bacteria was recently identified5. However, eukaryotic phytoplankton probably produce most of Earth's DMSP, yet no DMSP biosynthesis genes have been identified in any such organisms. Here we identify functional dsyB homologues, termed DSYB, in many phytoplankton and corals. DSYB is a methylthiohydroxybutryate methyltransferase enzyme localized in the chloroplasts and mitochondria of the haptophyte Prymnesium parvum, and stable isotope tracking experiments support these organelles as sites of DMSP synthesis. DSYB transcription levels increased with DMSP concentrations in different phytoplankton and were indicative of intracellular DMSP. Identification of the eukaryotic DSYB sequences, along with bacterial dsyB, provides the first molecular tools to predict the relative contributions of eukaryotes and prokaryotes to global DMSP production. Furthermore, evolutionary analysis suggests that eukaryotic DSYB originated in bacteria and was passed to eukaryotes early in their evolution.	en_US
dc.relation	http://purl.org/au-research/grants/arc/DE160100636
dc.relation.ispartof	Nature Microbiology	en_US
dc.relation.isbasedon	10.1038/s41564-018-0119-5	en_US
dc.subject.mesh	Mitochondria	en_US
dc.subject.mesh	Chloroplasts	en_US
dc.subject.mesh	Phytoplankton	en_US
dc.subject.mesh	Dinoflagellida	en_US
dc.subject.mesh	Diatoms	en_US
dc.subject.mesh	Sulfonium Compounds	en_US
dc.subject.mesh	Methyltransferases	en_US
dc.subject.mesh	Haptophyta	en_US
dc.title	DSYB catalyses the key step of dimethylsulfoniopropionate biosynthesis in many phytoplankton	en_US
dc.type	Journal Article
utslib.citation.volume	4	en_US
utslib.citation.volume	3	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	1108 Medical Microbiology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	open_access
pubs.issue	4	en_US
pubs.publication-status	Published	en_US
pubs.volume	3	en_US

Abstract:

© 2018 The Author(s). Dimethylsulfoniopropionate (DMSP) is a globally important organosulfur molecule and the major precursor for dimethyl sulfide. These compounds are important info-chemicals, key nutrients for marine microorganisms, and are involved in global sulfur cycling, atmospheric chemistry and cloud formation1-3. DMSP production was thought to be confined to eukaryotes, but heterotrophic bacteria can also produce DMSP through the pathway used by most phytoplankton4, and the DsyB enzyme catalysing the key step of this pathway in bacteria was recently identified5. However, eukaryotic phytoplankton probably produce most of Earth's DMSP, yet no DMSP biosynthesis genes have been identified in any such organisms. Here we identify functional dsyB homologues, termed DSYB, in many phytoplankton and corals. DSYB is a methylthiohydroxybutryate methyltransferase enzyme localized in the chloroplasts and mitochondria of the haptophyte Prymnesium parvum, and stable isotope tracking experiments support these organelles as sites of DMSP synthesis. DSYB transcription levels increased with DMSP concentrations in different phytoplankton and were indicative of intracellular DMSP. Identification of the eukaryotic DSYB sequences, along with bacterial dsyB, provides the first molecular tools to predict the relative contributions of eukaryotes and prokaryotes to global DMSP production. Furthermore, evolutionary analysis suggests that eukaryotic DSYB originated in bacteria and was passed to eukaryotes early in their evolution.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/122416