A novel immunomodulatory function of neutrophils on rhinovirus-Activated monocytes in vitro

Tang, FSM; Hansbro, PM; Burgess, JK; Ammit, AJ; Baines, KJ; Oliver, BG

A novel immunomodulatory function of neutrophils on rhinovirus-Activated monocytes in vitro

Tang, FSM Hansbro, PM

Burgess, JK Ammit, AJ

Baines, KJ Oliver, BG

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Publication Type:: Journal Article
Citation:: Thorax, 2016, 71 (11), pp. 1039 - 1049
Issue Date:: 2016-11-01

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Field	Value	Language
dc.contributor.author	Tang, FSM	en_US
dc.contributor.author	Hansbro, PM https://orcid.org/0000-0002-4741-3035	en_US
dc.contributor.author	Burgess, JK	en_US
dc.contributor.author	Ammit, AJ https://orcid.org/0000-0003-0555-2544	en_US
dc.contributor.author	Baines, KJ	en_US
dc.contributor.author	Oliver, BG https://orcid.org/0000-0002-7122-9262	en_US
dc.date.available	2016-05-15	en_US
dc.date.issued	2016-11-01	en_US
dc.identifier.citation	Thorax, 2016, 71 (11), pp. 1039 - 1049	en_US
dc.identifier.issn	0040-6376	en_US
dc.identifier.uri	http://hdl.handle.net/10453/122796
dc.description.abstract	© 2016 Published by the BMJ Publishing Group Limited. Background Rhinovirus (RV) infections are the major precipitant of asthma exacerbations. While neutrophilic lung inflammation occurs during such infections, its role remains unclear. Neutrophilic inflammation is associated with increased asthma severity and steroid refractory disease. Neutrophils are vital for controlling infections but also have immunomodulatory functions. Previously, we found that neutrophils respond to viral mimetics but not replication competent RV. We aimed to investigate if neutrophils are activated and/or modulate immune responses of monocytes during RV16 infection. Methods Primary human monocytes and autologous neutrophils were cocultured with or without RV16, in direct contact or separated by transwells. RV16-stimulated monocytes were also exposed to lysed neutrophils, neutrophil membrane components or soluble neutrophil intracellular components. Interleukin 6 (IL-6) and C-X-C motif (CXC)L8 mRNA and proteins were measured by quantitative PCR and ELISA at 24â €..hours. Results RV16 induced IL-6 and CXCL8 in monocytes, but not neutrophils. RV16-induced IL-6 and CXCL8 from monocytes was reduced in the presence of live neutrophils. Transwell separation abolished the inhibitory effects. Lysed neutrophils inhibited RV16-induced IL-6 and CXCL8 from monocytes. Neutrophil intracellular components alone effectively inhibited RV16-induced monocyte-derived IL-6 and CXCL8. Neutrophil intracellular components reduced RV16-induced IL-6 and CXCL8 mRNA in monocytes. Conclusions Cell contact between monocytes and neutrophils is required, and preformed neutrophil mediator(s) are likely to be involved in the suppression of cytokine mRNA and protein production. This study demonstrates a novel regulatory function of neutrophils on RV-Activated monocytes in vitro, challenging the paradigm that neutrophils are predominantly proinflammatory.	en_US
dc.relation	http://purl.org/au-research/grants/nhmrc/APP1110368
dc.relation	http://purl.org/au-research/grants/nhmrc/APP1104704
dc.relation	http://purl.org/au-research/grants/nhmrc/APP1026880
dc.relation.ispartof	Thorax	en_US
dc.relation.isbasedon	10.1136/thoraxjnl-2015-207781	en_US
dc.subject.classification	Respiratory System	en_US
dc.subject.mesh	Neutrophils	en_US
dc.subject.mesh	Monocytes	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Rhinovirus	en_US
dc.subject.mesh	Picornaviridae Infections	en_US
dc.subject.mesh	Inflammation	en_US
dc.subject.mesh	Interleukin-8	en_US
dc.subject.mesh	Interleukin-6	en_US
dc.subject.mesh	Enzyme-Linked Immunosorbent Assay	en_US
dc.subject.mesh	Coculture Techniques	en_US
dc.subject.mesh	Polymerase Chain Reaction	en_US
dc.subject.mesh	In Vitro Techniques	en_US
dc.title	A novel immunomodulatory function of neutrophils on rhinovirus-Activated monocytes in vitro	en_US
dc.type	Journal Article
utslib.citation.volume	11	en_US
utslib.citation.volume	71	en_US
utslib.for	1103 Clinical Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
utslib.copyright.status	open_access
pubs.issue	11	en_US
pubs.publication-status	Published	en_US
pubs.volume	71	en_US

Abstract:

© 2016 Published by the BMJ Publishing Group Limited. Background Rhinovirus (RV) infections are the major precipitant of asthma exacerbations. While neutrophilic lung inflammation occurs during such infections, its role remains unclear. Neutrophilic inflammation is associated with increased asthma severity and steroid refractory disease. Neutrophils are vital for controlling infections but also have immunomodulatory functions. Previously, we found that neutrophils respond to viral mimetics but not replication competent RV. We aimed to investigate if neutrophils are activated and/or modulate immune responses of monocytes during RV16 infection. Methods Primary human monocytes and autologous neutrophils were cocultured with or without RV16, in direct contact or separated by transwells. RV16-stimulated monocytes were also exposed to lysed neutrophils, neutrophil membrane components or soluble neutrophil intracellular components. Interleukin 6 (IL-6) and C-X-C motif (CXC)L8 mRNA and proteins were measured by quantitative PCR and ELISA at 24â €..hours. Results RV16 induced IL-6 and CXCL8 in monocytes, but not neutrophils. RV16-induced IL-6 and CXCL8 from monocytes was reduced in the presence of live neutrophils. Transwell separation abolished the inhibitory effects. Lysed neutrophils inhibited RV16-induced IL-6 and CXCL8 from monocytes. Neutrophil intracellular components alone effectively inhibited RV16-induced monocyte-derived IL-6 and CXCL8. Neutrophil intracellular components reduced RV16-induced IL-6 and CXCL8 mRNA in monocytes. Conclusions Cell contact between monocytes and neutrophils is required, and preformed neutrophil mediator(s) are likely to be involved in the suppression of cytokine mRNA and protein production. This study demonstrates a novel regulatory function of neutrophils on RV-Activated monocytes in vitro, challenging the paradigm that neutrophils are predominantly proinflammatory.

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http://hdl.handle.net/10453/122796