Distinct Bradyrhizbium communities nodulate legumes native to temperate and tropical monsoon Australia

Stepkowski, T; Watkin, E; McInnes, A; Gurda, D; Gracz, J; Steenkamp, ET

Distinct Bradyrhizbium communities nodulate legumes native to temperate and tropical monsoon Australia

Stepkowski, T Watkin, E McInnes, A

Gurda, D Gracz, J Steenkamp, ET

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Publication Type:: Journal Article
Citation:: Molecular Phylogenetics and Evolution, 2012, 63 (2), pp. 265 - 277
Issue Date:: 2012-05-01

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Field	Value	Language
dc.contributor.author	Stepkowski, T	en_US
dc.contributor.author	Watkin, E	en_US
dc.contributor.author	McInnes, A https://orcid.org/0000-0002-8340-3133	en_US
dc.contributor.author	Gurda, D	en_US
dc.contributor.author	Gracz, J	en_US
dc.contributor.author	Steenkamp, ET	en_US
dc.date.available	2011-12-19	en_US
dc.date.issued	2012-05-01	en_US
dc.identifier.citation	Molecular Phylogenetics and Evolution, 2012, 63 (2), pp. 265 - 277	en_US
dc.identifier.issn	1055-7903	en_US
dc.identifier.uri	http://hdl.handle.net/10453/123185
dc.description.abstract	Geographic isolation and growing climate aridity played major roles in the evolution of Australian legumes. It is likely that these two factors also impacted on the evolution of their root-nodule bacteria. To investigate this issue, we applied a multilocus sequence analysis (MLSA) approach to examine Bradyrhizobium isolates originating from temperate areas of Western Australia (WA) and the tropical-monsoon area of the Northern Territory (NT). The isolates were mostly collected from the nodules of legumes belonging to tribes, genera and species endemic or native to Australia. Phylogenetic analyses of sequences for the housekeeping atpD, dnaK, glnII, gyrB, recA and 16S rRNA genes and nodulation nodA gene revealed that most isolates belonged to groups that are distinct from non-Australian Bradyrhizobium isolates, which is in line with earlier studies based on 16S rRNA gene sequence analyses. Phylogenetic analysis of the nodA data allowed identification of five major Clades among the WA and NT isolates. All WA isolates grouped in a subgroup I.1 of Clade I with strains originating from temperate eastern Australia. In contrast, the NT isolates formed part of Clades I (subgroup I.2), III (subgroup III.3), IV, V and X. Of these nodA clades, Clade I, Clade IV, Clade X presumably have an Australian origin. Overall, these data demonstrate that the impact of geographic isolation of the Australian landmass is manifested by the presence of numerous unique clusters in housekeeping and nodulation gene trees. In addition, the intrinsic climate characteristics of temperate WA and tropical-monsoon NT were responsible for the formation of distinct legume communities selecting for unrelated Bradyrhizobium groups. © 2011 Elsevier Inc.	en_US
dc.relation.ispartof	Molecular Phylogenetics and Evolution	en_US
dc.relation.isbasedon	10.1016/j.ympev.2011.12.020	en_US
dc.subject.classification	Evolutionary Biology	en_US
dc.subject.mesh	Bradyrhizobium	en_US
dc.subject.mesh	Fabaceae	en_US
dc.subject.mesh	Acyltransferases	en_US
dc.subject.mesh	Bacterial Proteins	en_US
dc.subject.mesh	DNA, Bacterial	en_US
dc.subject.mesh	DNA, Ribosomal	en_US
dc.subject.mesh	RNA, Ribosomal, 16S	en_US
dc.subject.mesh	Sequence Analysis, DNA	en_US
dc.subject.mesh	Phylogeny	en_US
dc.subject.mesh	Symbiosis	en_US
dc.subject.mesh	Base Sequence	en_US
dc.subject.mesh	Multigene Family	en_US
dc.subject.mesh	Australia	en_US
dc.subject.mesh	Root Nodules, Plant	en_US
dc.subject.mesh	Multilocus Sequence Typing	en_US
dc.subject.mesh	Microbial Consortia	en_US
dc.title	Distinct Bradyrhizbium communities nodulate legumes native to temperate and tropical monsoon Australia	en_US
dc.type	Journal Article
utslib.citation.volume	2	en_US
utslib.citation.volume	63	en_US
utslib.for	0603 Evolutionary Biology	en_US
utslib.for	0604 Genetics	en_US
utslib.for	0608 Zoology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
utslib.copyright.status	closed_access
pubs.issue	2	en_US
pubs.publication-status	Published	en_US
pubs.volume	63	en_US

Abstract:

Geographic isolation and growing climate aridity played major roles in the evolution of Australian legumes. It is likely that these two factors also impacted on the evolution of their root-nodule bacteria. To investigate this issue, we applied a multilocus sequence analysis (MLSA) approach to examine Bradyrhizobium isolates originating from temperate areas of Western Australia (WA) and the tropical-monsoon area of the Northern Territory (NT). The isolates were mostly collected from the nodules of legumes belonging to tribes, genera and species endemic or native to Australia. Phylogenetic analyses of sequences for the housekeeping atpD, dnaK, glnII, gyrB, recA and 16S rRNA genes and nodulation nodA gene revealed that most isolates belonged to groups that are distinct from non-Australian Bradyrhizobium isolates, which is in line with earlier studies based on 16S rRNA gene sequence analyses. Phylogenetic analysis of the nodA data allowed identification of five major Clades among the WA and NT isolates. All WA isolates grouped in a subgroup I.1 of Clade I with strains originating from temperate eastern Australia. In contrast, the NT isolates formed part of Clades I (subgroup I.2), III (subgroup III.3), IV, V and X. Of these nodA clades, Clade I, Clade IV, Clade X presumably have an Australian origin. Overall, these data demonstrate that the impact of geographic isolation of the Australian landmass is manifested by the presence of numerous unique clusters in housekeeping and nodulation gene trees. In addition, the intrinsic climate characteristics of temperate WA and tropical-monsoon NT were responsible for the formation of distinct legume communities selecting for unrelated Bradyrhizobium groups. © 2011 Elsevier Inc.

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http://hdl.handle.net/10453/123185