Elemental bio-imaging using laser ablation-triple quadrupole-ICP-MS

Publication Type:
Journal Article
Citation:
Journal of Analytical Atomic Spectrometry, 2016, 31 (1), pp. 197 - 202
Issue Date:
2016-01-01
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© 2016 The Royal Society of Chemistry. Elemental bio-imaging (EBI) of trace metal distributions in tissue sections is typically performed by laser ablation-single quadrupole-inductively coupled plasma-mass spectrometry (LA-ICP-SQ-MS) in which the sensitivity of biologically relevant elements such as Fe may be inhibited by polyatomic interferences. The triple quadrupole ICP-MS (ICP-QQQ-MS) is designed to reduce interferences and was examined for its potential application to EBI. Three tune modes; a no-gas, an H2, and an O2/H2tune were developed and investigated for their suitability. All three performed well with high signal to noise ratios and low limits of detection. The O2/H2tune was applied to a prostate cancer biopsy sample, with high levels of Zn found in the area correlating with the cancer. The oxygen mass shift was applied to image P and Se in a mouse brain. The unique focussing properties of the ICP-QQQ-MS and the ability to use mixed cell gases provided sensitive analyses of all measured isotopes with the O2/H2tune, including those unaffected by mass shift reactions.
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