Physiological responses of Manila clams Venerupis (=Ruditapes) philippinarum with varying parasite Perkinsus olseni burden to toxic algal Alexandrium ostenfeldii exposure

Lassudrie, M; Soudant, P; Richard, G; Henry, N; Medhioub, W; da Silva, PM; Donval, A; Bunel, M; Le Goïc, N; Lambert, C; de Montaudouin, X; Fabioux, C; Hégaret, H

Physiological responses of Manila clams Venerupis (=Ruditapes) philippinarum with varying parasite Perkinsus olseni burden to toxic algal Alexandrium ostenfeldii exposure

Soudant, P Richard, G Henry, N Medhioub, W da Silva, PM Donval, A Bunel, M Le Goïc, N Lambert, C de Montaudouin, X Fabioux, C Hégaret, H

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Publication Type:: Journal Article
Citation:: Aquatic Toxicology, 2014, 154 pp. 27 - 38
Issue Date:: 2014-01-01

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Field	Value	Language
dc.contributor.author	Lassudrie, M https://orcid.org/0000-0002-7004-926X	en_US
dc.contributor.author	Soudant, P	en_US
dc.contributor.author	Richard, G	en_US
dc.contributor.author	Henry, N	en_US
dc.contributor.author	Medhioub, W	en_US
dc.contributor.author	da Silva, PM	en_US
dc.contributor.author	Donval, A	en_US
dc.contributor.author	Bunel, M	en_US
dc.contributor.author	Le Goïc, N	en_US
dc.contributor.author	Lambert, C	en_US
dc.contributor.author	de Montaudouin, X	en_US
dc.contributor.author	Fabioux, C	en_US
dc.contributor.author	Hégaret, H	en_US
dc.date.available	2014-05-05	en_US
dc.date.issued	2014-01-01	en_US
dc.identifier.citation	Aquatic Toxicology, 2014, 154 pp. 27 - 38	en_US
dc.identifier.issn	0166-445X	en_US
dc.identifier.uri	http://hdl.handle.net/10453/123430
dc.description.abstract	Manila clam stock from Arcachon Bay, France, is declining, as is commercial harvest. To understand the role of environmental biotic interactions in this decrease, effects of a toxic dinoflagellate, Alexandrium ostenfeldii, which blooms regularly in Arcachon bay, and the interaction with perkinsosis on clam physiology were investigated. Manila clams from Arcachon Bay, with variable natural levels of perkinsosis, were exposed for seven days to a mix of the nutritious microalga T-Iso and the toxic dinoflagellate A. ostenfeldii, a producer of spirolides, followed by seven days of depuration fed only T-Iso. Following sacrifice and quantification of protozoan parasite Perkinsus olseni burden, clams were divided into two groups according to intensity of the infection ("Light-Moderate" and "Moderate-Heavy"). Hemocyte and plasma responses, digestive enzyme activities, antioxidant enzyme activities in gills, and histopathological responses were analyzed. Reactive oxygen species (ROS) production in hemocytes and catalase (CAT) activity in gills increased with P. olseni intensity of infection in control clams fed T-Iso, but did not vary among A. ostenfeldii-exposed clams. Exposure to A. ostenfeldii caused tissue alterations associated with an inflammatory response and modifications in hemocyte morphology. In the gills, superoxide dismutase (SOD) activity decreased, and an increase in brown cell occurrence was seen, suggesting oxidative stress. Observations of hemocytes and brown cells in tissues during exposure and depuration suggest involvement of both cell types in detoxication processes. Results suggest that exposure to A. ostenfeldii disrupted the pro-/anti-oxidant response of clams to heavy P. olseni intensity. In addition, depressed mitochondrial membrane potential (MMP) in hemocytes of clams exposed to A. ostenfeldii suggests that mitochondrial functions are regulated to maintain homeostasis of digestive enzyme activity and condition index. © 2014 Elsevier B.V.	en_US
dc.relation.ispartof	Aquatic Toxicology	en_US
dc.relation.isbasedon	10.1016/j.aquatox.2014.05.002	en_US
dc.subject.classification	Toxicology	en_US
dc.subject.mesh	Hemocytes	en_US
dc.subject.mesh	Mitochondria	en_US
dc.subject.mesh	Gills	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Dinoflagellida	en_US
dc.subject.mesh	Reactive Oxygen Species	en_US
dc.subject.mesh	Spiro Compounds	en_US
dc.subject.mesh	Oxidoreductases	en_US
dc.subject.mesh	Marine Toxins	en_US
dc.subject.mesh	Water Pollutants, Chemical	en_US
dc.subject.mesh	Enzyme Activation	en_US
dc.subject.mesh	France	en_US
dc.subject.mesh	Bivalvia	en_US
dc.subject.mesh	Alveolata	en_US
dc.subject.mesh	Parasite Load	en_US
dc.title	Physiological responses of Manila clams Venerupis (=Ruditapes) philippinarum with varying parasite Perkinsus olseni burden to toxic algal Alexandrium ostenfeldii exposure	en_US
dc.type	Journal Article
utslib.citation.volume	154	en_US
utslib.for	0602 Ecology	en_US
utslib.for	03 Chemical Sciences	en_US
utslib.for	05 Environmental Sciences	en_US
utslib.for	06 Biological Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
utslib.copyright.status	closed_access
pubs.publication-status	Published	en_US
pubs.volume	154	en_US

Abstract:

Manila clam stock from Arcachon Bay, France, is declining, as is commercial harvest. To understand the role of environmental biotic interactions in this decrease, effects of a toxic dinoflagellate, Alexandrium ostenfeldii, which blooms regularly in Arcachon bay, and the interaction with perkinsosis on clam physiology were investigated. Manila clams from Arcachon Bay, with variable natural levels of perkinsosis, were exposed for seven days to a mix of the nutritious microalga T-Iso and the toxic dinoflagellate A. ostenfeldii, a producer of spirolides, followed by seven days of depuration fed only T-Iso. Following sacrifice and quantification of protozoan parasite Perkinsus olseni burden, clams were divided into two groups according to intensity of the infection ("Light-Moderate" and "Moderate-Heavy"). Hemocyte and plasma responses, digestive enzyme activities, antioxidant enzyme activities in gills, and histopathological responses were analyzed. Reactive oxygen species (ROS) production in hemocytes and catalase (CAT) activity in gills increased with P. olseni intensity of infection in control clams fed T-Iso, but did not vary among A. ostenfeldii-exposed clams. Exposure to A. ostenfeldii caused tissue alterations associated with an inflammatory response and modifications in hemocyte morphology. In the gills, superoxide dismutase (SOD) activity decreased, and an increase in brown cell occurrence was seen, suggesting oxidative stress. Observations of hemocytes and brown cells in tissues during exposure and depuration suggest involvement of both cell types in detoxication processes. Results suggest that exposure to A. ostenfeldii disrupted the pro-/anti-oxidant response of clams to heavy P. olseni intensity. In addition, depressed mitochondrial membrane potential (MMP) in hemocytes of clams exposed to A. ostenfeldii suggests that mitochondrial functions are regulated to maintain homeostasis of digestive enzyme activity and condition index. © 2014 Elsevier B.V.

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http://hdl.handle.net/10453/123430