An improved liquid chromatography tandem mass spectrometry (LC–MS/MS) method for quantification of dexmedetomidine concentrations in samples of human plasma

Moosavi, SM; Shekar, K; Fraser, JF; Smith, MT; Ghassabian, S

An improved liquid chromatography tandem mass spectrometry (LC–MS/MS) method for quantification of dexmedetomidine concentrations in samples of human plasma

Moosavi, SM

Shekar, K Fraser, JF Smith, MT Ghassabian, S

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Publication Type:: Journal Article
Citation:: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 2018, 1073 pp. 118 - 122
Issue Date:: 2018-01-15

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Field	Value	Language
dc.contributor.author	Moosavi, SM https://orcid.org/0000-0002-0905-3825	en_US
dc.contributor.author	Shekar, K	en_US
dc.contributor.author	Fraser, JF	en_US
dc.contributor.author	Smith, MT	en_US
dc.contributor.author	Ghassabian, S	en_US
dc.date.available	2017-12-03	en_US
dc.date.issued	2018-01-15	en_US
dc.identifier.citation	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 2018, 1073 pp. 118 - 122	en_US
dc.identifier.issn	1570-0232	en_US
dc.identifier.uri	http://hdl.handle.net/10453/123453
dc.description.abstract	© 2017 Elsevier B.V. Dexmedetomidine (DMET) is a sedative, analgesic and anxiolytic with minimum adverse respiratory effects. An LC–MS/MS bioanalytical method has been developed and validated to accurately measure DMET concentrations in samples of human plasma. The method overcomes difficulties in the extraction and quantification of DMET due to the fact that it binds strongly to glass and plastic tubes, as well as solid phase extraction (SPE) cartridges. Human plasma (50 μL) was mixed with the internal standard (IS) (DMET-d4) solution (100 μL) and 0.1% formic acid (50 μL) and extracted using Oasis HLB 1 CC (30 mg) solid phase extraction (SPE) cartridges (Waters®). The glass tubes were coated with bovine serum albumin (BSA) 0.5% (20 μL) before eluting DMET and the IS. After evaporation under nitrogen at room temperature, the analytes were reconstituted in 20% acetonitrile in 0.1% formic acid in water and transferred to silanized glass vials. An electrospray ionisation (ESI) mass spectrometry method in positive mode was created and the precursor/product transitions (m/z) were 201.1 → 95.0 (DMET) and 204.9 → 99.0 (IS). The method was robust and fully validated based on the 2012 EMEA guideline for bioanalytical method validation in the concentration range of 0.5–20 ng/mL. Using this assay, we showed that DMET binds strongly to Extracorporeal Membrane Oxygenation (ECMO) circuits, consistent with expectations for small lipophilic compounds.	en_US
dc.relation.ispartof	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences	en_US
dc.relation.isbasedon	10.1016/j.jchromb.2017.12.006	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Dexmedetomidine	en_US
dc.subject.mesh	Chromatography, Liquid	en_US
dc.subject.mesh	Linear Models	en_US
dc.subject.mesh	Sensitivity and Specificity	en_US
dc.subject.mesh	Reproducibility of Results	en_US
dc.subject.mesh	Tandem Mass Spectrometry	en_US
dc.subject.mesh	Solid Phase Extraction	en_US
dc.title	An improved liquid chromatography tandem mass spectrometry (LC–MS/MS) method for quantification of dexmedetomidine concentrations in samples of human plasma	en_US
dc.type	Journal Article
utslib.citation.volume	1073	en_US
utslib.for	0301 Analytical Chemistry	en_US
utslib.for	0601 Biochemistry and Cell Biology	en_US
utslib.for	1115 Pharmacology and Pharmaceutical Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
utslib.copyright.status	closed_access
pubs.publication-status	Published	en_US
pubs.volume	1073	en_US

Abstract:

© 2017 Elsevier B.V. Dexmedetomidine (DMET) is a sedative, analgesic and anxiolytic with minimum adverse respiratory effects. An LC–MS/MS bioanalytical method has been developed and validated to accurately measure DMET concentrations in samples of human plasma. The method overcomes difficulties in the extraction and quantification of DMET due to the fact that it binds strongly to glass and plastic tubes, as well as solid phase extraction (SPE) cartridges. Human plasma (50 μL) was mixed with the internal standard (IS) (DMET-d4) solution (100 μL) and 0.1% formic acid (50 μL) and extracted using Oasis HLB 1 CC (30 mg) solid phase extraction (SPE) cartridges (Waters®). The glass tubes were coated with bovine serum albumin (BSA) 0.5% (20 μL) before eluting DMET and the IS. After evaporation under nitrogen at room temperature, the analytes were reconstituted in 20% acetonitrile in 0.1% formic acid in water and transferred to silanized glass vials. An electrospray ionisation (ESI) mass spectrometry method in positive mode was created and the precursor/product transitions (m/z) were 201.1 → 95.0 (DMET) and 204.9 → 99.0 (IS). The method was robust and fully validated based on the 2012 EMEA guideline for bioanalytical method validation in the concentration range of 0.5–20 ng/mL. Using this assay, we showed that DMET binds strongly to Extracorporeal Membrane Oxygenation (ECMO) circuits, consistent with expectations for small lipophilic compounds.

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http://hdl.handle.net/10453/123453