Redefining the sponge-symbiont acquisition paradigm: sponge microbes exhibit chemotaxis towards host-derived compounds

Tout, J; Astudillo-García, C; Taylor, MW; Tyson, GW; Stocker, R; Ralph, PJ; Seymour, JR; Webster, NS

Redefining the sponge-symbiont acquisition paradigm: sponge microbes exhibit chemotaxis towards host-derived compounds

Tout, J Astudillo-García, C Taylor, MW Tyson, GW Stocker, R Ralph, PJ

Seymour, JR

Webster, NS

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Publication Type:: Journal Article
Citation:: Environmental Microbiology Reports, 2017, 9 (6), pp. 750 - 755
Issue Date:: 2017-12-01

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Field	Value	Language
dc.contributor.author	Tout, J	en_US
dc.contributor.author	Astudillo-García, C	en_US
dc.contributor.author	Taylor, MW	en_US
dc.contributor.author	Tyson, GW	en_US
dc.contributor.author	Stocker, R	en_US
dc.contributor.author	Ralph, PJ https://orcid.org/0000-0002-3103-7346	en_US
dc.contributor.author	Seymour, JR https://orcid.org/0000-0002-3745-6541	en_US
dc.contributor.author	Webster, NS	en_US
dc.date.available	2017-08-31	en_US
dc.date.issued	2017-12-01	en_US
dc.identifier.citation	Environmental Microbiology Reports, 2017, 9 (6), pp. 750 - 755	en_US
dc.identifier.uri	http://hdl.handle.net/10453/128691
dc.description.abstract	© 2017 Society for Applied Microbiology and John Wiley & Sons Ltd Marine sponges host stable and species-specific microbial symbionts that are thought to be acquired and maintained by the host through a combination of vertical transmission and filtration from the surrounding seawater. To assess whether the microbial symbionts also actively contribute to the establishment of these symbioses, we performed in situ experiments on Orpheus Island, Great Barrier Reef, to quantify the chemotactic responses of natural populations of seawater microorganisms towards cellular extracts of the reef sponge Rhopaloeides odorabile. Flow cytometry analysis revealed significant levels of microbial chemotaxis towards R. odorabile extracts and 16S rRNA gene amplicon sequencing showed enrichment of ‘sponge-specific’ microbial phylotypes, including a cluster within the Gemmatimonadetes and another within the Actinobacteria. These findings infer a potential mechanism for how sponges can acquire bacterial symbionts from the surrounding environment and suggest an active role of the symbionts in finding their host.	en_US
dc.relation	http://purl.org/au-research/grants/arc/FT130100218
dc.relation.ispartof	Environmental Microbiology Reports	en_US
dc.relation.isbasedon	10.1111/1758-2229.12591	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Porifera	en_US
dc.subject.mesh	Bacteria	en_US
dc.subject.mesh	Actinobacteria	en_US
dc.subject.mesh	RNA, Ribosomal, 16S	en_US
dc.subject.mesh	Flow Cytometry	en_US
dc.subject.mesh	Sequence Analysis, DNA	en_US
dc.subject.mesh	Seawater	en_US
dc.subject.mesh	Symbiosis	en_US
dc.subject.mesh	Chemotaxis	en_US
dc.subject.mesh	Queensland	en_US
dc.subject.mesh	Bacterial Physiological Phenomena	en_US
dc.title	Redefining the sponge-symbiont acquisition paradigm: sponge microbes exhibit chemotaxis towards host-derived compounds	en_US
dc.type	Journal Article
utslib.citation.volume	6	en_US
utslib.citation.volume	9	en_US
utslib.for	0602 Ecology	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	0603 Evolutionary Biology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	open_access
pubs.issue	6	en_US
pubs.publication-status	Published	en_US
pubs.volume	9	en_US

Abstract:

© 2017 Society for Applied Microbiology and John Wiley & Sons Ltd Marine sponges host stable and species-specific microbial symbionts that are thought to be acquired and maintained by the host through a combination of vertical transmission and filtration from the surrounding seawater. To assess whether the microbial symbionts also actively contribute to the establishment of these symbioses, we performed in situ experiments on Orpheus Island, Great Barrier Reef, to quantify the chemotactic responses of natural populations of seawater microorganisms towards cellular extracts of the reef sponge Rhopaloeides odorabile. Flow cytometry analysis revealed significant levels of microbial chemotaxis towards R. odorabile extracts and 16S rRNA gene amplicon sequencing showed enrichment of ‘sponge-specific’ microbial phylotypes, including a cluster within the Gemmatimonadetes and another within the Actinobacteria. These findings infer a potential mechanism for how sponges can acquire bacterial symbionts from the surrounding environment and suggest an active role of the symbionts in finding their host.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/123629