The relative availability of inorganic carbon and inorganic nitrogen influences the response of the dinoflagellate Protoceratium reticulatum to elevated CO<inf>2</inf>

Pierangelini, M; Raven, JA; Giordano, M

The relative availability of inorganic carbon and inorganic nitrogen influences the response of the dinoflagellate Protoceratium reticulatum to elevated CO<inf>2</inf>

Pierangelini, M Raven, JA

Giordano, M

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Publication Type:: Journal Article
Citation:: Journal of Phycology, 2017, 53 (2), pp. 298 - 307
Issue Date:: 2017-04-01

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Field	Value	Language
dc.contributor.author	Pierangelini, M	en_US
dc.contributor.author	Raven, JA https://orcid.org/0000-0002-2789-3297	en_US
dc.contributor.author	Giordano, M	en_US
dc.date.available	2016-07-23	en_US
dc.date.issued	2017-04-01	en_US
dc.identifier.citation	Journal of Phycology, 2017, 53 (2), pp. 298 - 307	en_US
dc.identifier.issn	0022-3646	en_US
dc.identifier.uri	http://hdl.handle.net/10453/123726
dc.description.abstract	© 2016 Phycological Society of America This work originates from three facts: (i) changes in CO2 availability influence metabolic processes in algal cells; (ii) Spatial and temporal variations of nitrogen availability cause repercussions on phytoplankton physiology; (iii) Growth and cell composition are dependent on the stoichiometry of nutritional resources. In this study, we assess whether the impact of rising pCO2 is influenced by N availability, through the impact that it would have on the C/N stoichiometry, in conditions of N sufficiency. Our experiments used the dinoflagellate Protoceratium reticulatum, which we cultured under three CO2 regimes (400, 1,000, and 5,000 ppmv, pH of 8.1) and either variable (the NO3− concentration was always 2.5 mmol · L−1) or constant (NO3− concentration varied to maintain the same Ci/NO3− ratio at all pCO2) Ci/NO3− ratio. Regardless of N availability, cells had higher specific growth rates, but lower cell dry weight and C and N quotas, at elevated CO2. The carbohydrate pool size and the C/N was unaltered in all treatments. The lipid content only decreased at high pCO2 at constant Ci/NO3− ratio. In the variable Ci/NO3− conditions, the relative abundance of Rubisco (and other proteins) also changed; this did not occur at constant Ci/NO3−. Thus, the biomass quality of P. reticulatum for grazers was affected by the Ci/NO3− ratio in the environment and not only by the pCO2, both with respect to the size of the main organic pools and the composition of the expressed proteome.	en_US
dc.relation.ispartof	Journal of Phycology	en_US
dc.relation.isbasedon	10.1111/jpy.12463	en_US
dc.subject.classification	Marine Biology & Hydrobiology	en_US
dc.subject.mesh	Dinoflagellida	en_US
dc.subject.mesh	Carbon Dioxide	en_US
dc.subject.mesh	Carbon	en_US
dc.subject.mesh	Nitrogen	en_US
dc.subject.mesh	Hydrogen-Ion Concentration	en_US
dc.title	The relative availability of inorganic carbon and inorganic nitrogen influences the response of the dinoflagellate Protoceratium reticulatum to elevated CO<inf>2</inf>	en_US
dc.type	Journal Article
utslib.citation.volume	2	en_US
utslib.citation.volume	53	en_US
utslib.for	0607 Plant Biology	en_US
utslib.for	0704 Fisheries Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
utslib.copyright.status	closed_access
pubs.issue	2	en_US
pubs.publication-status	Published	en_US
pubs.volume	53	en_US

Abstract:

© 2016 Phycological Society of America This work originates from three facts: (i) changes in CO2 availability influence metabolic processes in algal cells; (ii) Spatial and temporal variations of nitrogen availability cause repercussions on phytoplankton physiology; (iii) Growth and cell composition are dependent on the stoichiometry of nutritional resources. In this study, we assess whether the impact of rising pCO2 is influenced by N availability, through the impact that it would have on the C/N stoichiometry, in conditions of N sufficiency. Our experiments used the dinoflagellate Protoceratium reticulatum, which we cultured under three CO2 regimes (400, 1,000, and 5,000 ppmv, pH of 8.1) and either variable (the NO3− concentration was always 2.5 mmol · L−1) or constant (NO3− concentration varied to maintain the same Ci/NO3− ratio at all pCO2) Ci/NO3− ratio. Regardless of N availability, cells had higher specific growth rates, but lower cell dry weight and C and N quotas, at elevated CO2. The carbohydrate pool size and the C/N was unaltered in all treatments. The lipid content only decreased at high pCO2 at constant Ci/NO3− ratio. In the variable Ci/NO3− conditions, the relative abundance of Rubisco (and other proteins) also changed; this did not occur at constant Ci/NO3−. Thus, the biomass quality of P. reticulatum for grazers was affected by the Ci/NO3− ratio in the environment and not only by the pCO2, both with respect to the size of the main organic pools and the composition of the expressed proteome.

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http://hdl.handle.net/10453/123726