Combined Peptidomic and Proteomic Analysis of Electrically Stimulated and Manually Dissected Venom from the South American Bullet Ant Paraponera clavata

Aili, SR; Touchard, A; Petitclerc, F; Dejean, A; Orivel, J; Padula, MP; Escoubas, P; Nicholson, GM

Combined Peptidomic and Proteomic Analysis of Electrically Stimulated and Manually Dissected Venom from the South American Bullet Ant Paraponera clavata

Aili, SR Touchard, A Petitclerc, F Dejean, A Orivel, J Padula, MP

Escoubas, P Nicholson, GM

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Publication Type:: Journal Article
Citation:: Journal of Proteome Research, 2017, 16 (3), pp. 1339 - 1351
Issue Date:: 2017-03-03

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Field	Value	Language
dc.contributor.author	Aili, SR	en_US
dc.contributor.author	Touchard, A	en_US
dc.contributor.author	Petitclerc, F	en_US
dc.contributor.author	Dejean, A	en_US
dc.contributor.author	Orivel, J	en_US
dc.contributor.author	Padula, MP https://orcid.org/0000-0002-8283-0643	en_US
dc.contributor.author	Escoubas, P	en_US
dc.contributor.author	Nicholson, GM https://orcid.org/0000-0002-4277-4296	en_US
dc.date.issued	2017-03-03	en_US
dc.identifier.citation	Journal of Proteome Research, 2017, 16 (3), pp. 1339 - 1351	en_US
dc.identifier.issn	1535-3893	en_US
dc.identifier.uri	http://hdl.handle.net/10453/123916
dc.description.abstract	© 2017 American Chemical Society. Ants have evolved venoms rich in peptides and proteins used for predation, defense, and communication. However, they remain extremely understudied due to the minimal amount of venom secreted by each ant. The present study investigated the differences in the proteome and peptidome of the venom from the bullet ant, Paraponera clavata. Venom samples were collected from a single colony either by manual venom gland dissection or by electrical stimulation and were compared using proteomic methods. Venom proteins were separated by 2D-PAGE and identified by nanoLC-ESI-QTOF MS/MS. Venom peptides were initially separated using C18 reversed-phase high-performance liquid chromatography, then analyzed by MALDI-TOF MS. The proteomic analysis revealed numerous proteins that could be assigned a biological function (total 94), mainly as toxins, or roles in cell regulation and transport. This investigation found that ca. 73% of the proteins were common to venoms collected by the two methods. The peptidomic analysis revealed a large number of peptides (total 309) but with <20% shared by the two collection methods. There was also a marked difference between venoms obtained by venom gland dissection from different ant colonies. These findings demonstrate the rich composition and variability of P. clavata venom.	en_US
dc.relation.ispartof	Journal of Proteome Research	en_US
dc.relation.isbasedon	10.1021/acs.jproteome.6b00948	en_US
dc.subject.classification	Biochemistry & Molecular Biology	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Ants	en_US
dc.subject.mesh	Peptides	en_US
dc.subject.mesh	Insect Proteins	en_US
dc.subject.mesh	Ant Venoms	en_US
dc.subject.mesh	Electrophoresis, Gel, Two-Dimensional	en_US
dc.subject.mesh	Proteomics	en_US
dc.subject.mesh	Tandem Mass Spectrometry	en_US
dc.title	Combined Peptidomic and Proteomic Analysis of Electrically Stimulated and Manually Dissected Venom from the South American Bullet Ant Paraponera clavata	en_US
dc.type	Journal Article
utslib.citation.volume	3	en_US
utslib.citation.volume	16	en_US
utslib.for	0601 Biochemistry and Cell Biology	en_US
utslib.for	0301 Analytical Chemistry	en_US
utslib.for	03 Chemical Sciences	en_US
utslib.for	06 Biological Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
utslib.copyright.status	open_access
pubs.issue	3	en_US
pubs.publication-status	Published	en_US
pubs.volume	16	en_US

Abstract:

© 2017 American Chemical Society. Ants have evolved venoms rich in peptides and proteins used for predation, defense, and communication. However, they remain extremely understudied due to the minimal amount of venom secreted by each ant. The present study investigated the differences in the proteome and peptidome of the venom from the bullet ant, Paraponera clavata. Venom samples were collected from a single colony either by manual venom gland dissection or by electrical stimulation and were compared using proteomic methods. Venom proteins were separated by 2D-PAGE and identified by nanoLC-ESI-QTOF MS/MS. Venom peptides were initially separated using C18 reversed-phase high-performance liquid chromatography, then analyzed by MALDI-TOF MS. The proteomic analysis revealed numerous proteins that could be assigned a biological function (total 94), mainly as toxins, or roles in cell regulation and transport. This investigation found that ca. 73% of the proteins were common to venoms collected by the two methods. The peptidomic analysis revealed a large number of peptides (total 309) but with <20% shared by the two collection methods. There was also a marked difference between venoms obtained by venom gland dissection from different ant colonies. These findings demonstrate the rich composition and variability of P. clavata venom.

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http://hdl.handle.net/10453/123916