RCH51, a multiply antibiotic-resistant Acinetobacter baumannii ST103IP isolate, carries resistance genes in three plasmids, including a novel potentially conjugative plasmid carrying oxa235 in transposon Tn6252

Hamidian, M; Nigro, SJ; Hartstein, RM; Hall, RM

RCH51, a multiply antibiotic-resistant Acinetobacter baumannii ST103IP isolate, carries resistance genes in three plasmids, including a novel potentially conjugative plasmid carrying oxa235 in transposon Tn6252

Hamidian, M

Nigro, SJ Hartstein, RM Hall, RM

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Publication Type:: Journal Article
Citation:: Journal of Antimicrobial Chemotherapy, 2017, 72 (7), pp. 1907 - 1910
Issue Date:: 2017-07-01

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Field	Value	Language
dc.contributor.author	Hamidian, M https://orcid.org/0000-0002-3614-7261	en_US
dc.contributor.author	Nigro, SJ	en_US
dc.contributor.author	Hartstein, RM	en_US
dc.contributor.author	Hall, RM	en_US
dc.date.available	2020-05-25T19:04:44Z
dc.date.issued	2017-07-01	en_US
dc.identifier.citation	Journal of Antimicrobial Chemotherapy, 2017, 72 (7), pp. 1907 - 1910	en_US
dc.identifier.issn	0305-7453	en_US
dc.identifier.uri	http://hdl.handle.net/10453/123976
dc.description.abstract	© The Author 2017. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. Objectives: To determine the identity and context of genes conferring antibiotic resistance in a sporadic multiply antibiotic-resistant Acinetobacter baumannii recovered at Royal Children's Hospital, Brisbane. Methods: The antibiotic resistance phenotype for 23 antibiotics was determined using disc diffusion or MIC determination. The whole-genome sequence of RCH51 was determined using the Illumina HiSeq platform. Antibiotic resistance determinants were identified using ResFinder. Plasmids were recovered by transformation. Results: Isolate RCH51 belongs to the uncommon STs ST103IP (7-3-2-1-7-1-4) and ST514OX (1-52-29-28-18- 114-7). It was found to be resistant to sulfamethoxazole, tetracycline, gentamicin, tobramycin and kanamycin and also exhibited reduced susceptibility to imipenem(MIC 2 mg/L) and meropenem (MIC 6 mg/L). RCH51 carries the oxa235, sul2, floR, aadB and tet39 resistance genes, all located on plasmids. The largest of the three plasmids, pRCH51-3, is 52 789 bp and carries oxa235 in the ISAba1-bounded transposon Tn6252, as well as sul2 and floR. pRCH51-3 represents a new A. baumannii plasmid family that is potentially conjugative as it contains several genes predicted to encode transfer functions. However, conjugation of pRCH51-3 was not detected. The aadB and tet39 resistance genes were each found in small plasmids identical to the known plasmids pRAY*-v1 and pRCH52-1, respectively. Conclusions: The resistance gene complement of RCH51 was found in three plasmids. pRCH51-3, which carries the oxa235, sul2 and floR resistance genes, represents a new, potentially conjugative A. baumannii plasmid type.	en_US
dc.relation.ispartof	Journal of Antimicrobial Chemotherapy	en_US
dc.relation.isbasedon	10.1093/jac/dkx069	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Acinetobacter baumannii	en_US
dc.subject.mesh	Acinetobacter Infections	en_US
dc.subject.mesh	beta-Lactamases	en_US
dc.subject.mesh	Gentamicins	en_US
dc.subject.mesh	Tobramycin	en_US
dc.subject.mesh	DNA, Bacterial	en_US
dc.subject.mesh	DNA Transposable Elements	en_US
dc.subject.mesh	Anti-Bacterial Agents	en_US
dc.subject.mesh	Microbial Sensitivity Tests	en_US
dc.subject.mesh	Sequence Analysis, DNA	en_US
dc.subject.mesh	Drug Resistance, Multiple, Bacterial	en_US
dc.subject.mesh	Conjugation, Genetic	en_US
dc.subject.mesh	Genomic Islands	en_US
dc.subject.mesh	Genome, Bacterial	en_US
dc.subject.mesh	Plasmids	en_US
dc.title	RCH51, a multiply antibiotic-resistant Acinetobacter baumannii ST103IP isolate, carries resistance genes in three plasmids, including a novel potentially conjugative plasmid carrying oxa235 in transposon Tn6252	en_US
dc.type	Journal Article
utslib.citation.volume	7	en_US
utslib.citation.volume	72	en_US
utslib.for	1115 Pharmacology and Pharmaceutical Sciences	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	1108 Medical Microbiology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	open_access
pubs.issue	7	en_US
pubs.publication-status	Published	en_US
pubs.volume	72	en_US

Abstract:

© The Author 2017. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. Objectives: To determine the identity and context of genes conferring antibiotic resistance in a sporadic multiply antibiotic-resistant Acinetobacter baumannii recovered at Royal Children's Hospital, Brisbane. Methods: The antibiotic resistance phenotype for 23 antibiotics was determined using disc diffusion or MIC determination. The whole-genome sequence of RCH51 was determined using the Illumina HiSeq platform. Antibiotic resistance determinants were identified using ResFinder. Plasmids were recovered by transformation. Results: Isolate RCH51 belongs to the uncommon STs ST103IP (7-3-2-1-7-1-4) and ST514OX (1-52-29-28-18- 114-7). It was found to be resistant to sulfamethoxazole, tetracycline, gentamicin, tobramycin and kanamycin and also exhibited reduced susceptibility to imipenem(MIC 2 mg/L) and meropenem (MIC 6 mg/L). RCH51 carries the oxa235, sul2, floR, aadB and tet39 resistance genes, all located on plasmids. The largest of the three plasmids, pRCH51-3, is 52 789 bp and carries oxa235 in the ISAba1-bounded transposon Tn6252, as well as sul2 and floR. pRCH51-3 represents a new A. baumannii plasmid family that is potentially conjugative as it contains several genes predicted to encode transfer functions. However, conjugation of pRCH51-3 was not detected. The aadB and tet39 resistance genes were each found in small plasmids identical to the known plasmids pRAY*-v1 and pRCH52-1, respectively. Conclusions: The resistance gene complement of RCH51 was found in three plasmids. pRCH51-3, which carries the oxa235, sul2 and floR resistance genes, represents a new, potentially conjugative A. baumannii plasmid type.

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http://hdl.handle.net/10453/123976