pIP40a, a type 1 IncC plasmid from 1969 carries the integrative element GIsul2 and a novel class II mercury resistance transposon

Harmer, CJ; Hamidian, M; Hall, RM

pIP40a, a type 1 IncC plasmid from 1969 carries the integrative element GIsul2 and a novel class II mercury resistance transposon

Harmer, CJ Hamidian, M

Hall, RM

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Publication Type:: Journal Article
Citation:: Plasmid, 2017, 92 pp. 17 - 25
Issue Date:: 2017-07-01

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Field	Value	Language
dc.contributor.author	Harmer, CJ	en_US
dc.contributor.author	Hamidian, M https://orcid.org/0000-0002-3614-7261	en_US
dc.contributor.author	Hall, RM	en_US
dc.date.available	2020-05-25T19:16:03Z
dc.date.issued	2017-07-01	en_US
dc.identifier.citation	Plasmid, 2017, 92 pp. 17 - 25	en_US
dc.identifier.issn	0147-619X	en_US
dc.identifier.uri	http://hdl.handle.net/10453/123978
dc.description.abstract	© 2017 Elsevier Inc. The 167.5 kb sequence of the conjugative IncC plasmid pIP40a, isolated from a Pseudomonas aeruginosa in 1969, was analysed. pIP40a confers resistance to kanamycin, neomycin, ampicillin, sulphonamides and mercuric ions, and several insertions in a type 1 IncC backbone were found, including copies of IS3, Tn1000 and a novel mercury resistance transposon, Tn6182. The antibiotic resistance genes were in two locations. Tn6023, containing the aphA1 kanamycin and neomycin resistance gene, is in a partial copy of Tn1/Tn2/Tn3 (blaTEM, ampicillin resistance) in the kfrA gene, and the sul2 sulphonamide resistance gene is in the integrative element GIsul2 in the position of ARI-B islands. The 11.5 kb class II transposon Tn6182 is only distantly related to other class II transposons, with at most 33% identity between the TnpA of Tn6182 and TnpA of other group members. In addition, the inverted repeats are 37 bp rather than 38 bp, and the likely resolution enzyme is a tyrosine recombinase (TnpI). Re-annotation of GIsul2 revealed genes predicted to confer resistance to arsenate and arsenite, but resistance was not detected. The location of GIsul2 confirms it as the progenitor of the ARI-B configurations seen in many IncC plasmids isolated more recently. However, GIsul2 has integrated at the same site in type 1 and type 2 IncC plasmids, indicating that it targets this site. Analysis of the distribution of GIsul2 revealed that it in addition to its chromosomal integration site at the 3′-end of the guaA gene, it has also integrated into other plasmids, increasing its mobility.	en_US
dc.relation.ispartof	Plasmid	en_US
dc.relation.isbasedon	10.1016/j.plasmid.2017.05.004	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Pseudomonas aeruginosa	en_US
dc.subject.mesh	Escherichia coli	en_US
dc.subject.mesh	Mercuric Chloride	en_US
dc.subject.mesh	Bacterial Proteins	en_US
dc.subject.mesh	DNA, Bacterial	en_US
dc.subject.mesh	DNA Transposable Elements	en_US
dc.subject.mesh	Anti-Bacterial Agents	en_US
dc.subject.mesh	Microbial Sensitivity Tests	en_US
dc.subject.mesh	Sequence Analysis, DNA	en_US
dc.subject.mesh	Phylogeny	en_US
dc.subject.mesh	Drug Resistance, Multiple, Bacterial	en_US
dc.subject.mesh	Base Sequence	en_US
dc.subject.mesh	Conserved Sequence	en_US
dc.subject.mesh	Plasmids	en_US
dc.title	pIP40a, a type 1 IncC plasmid from 1969 carries the integrative element GIsul2 and a novel class II mercury resistance transposon	en_US
dc.type	Journal Article
utslib.citation.volume	92	en_US
utslib.for	0604 Genetics	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	open_access
pubs.publication-status	Published	en_US
pubs.volume	92	en_US

Abstract:

© 2017 Elsevier Inc. The 167.5 kb sequence of the conjugative IncC plasmid pIP40a, isolated from a Pseudomonas aeruginosa in 1969, was analysed. pIP40a confers resistance to kanamycin, neomycin, ampicillin, sulphonamides and mercuric ions, and several insertions in a type 1 IncC backbone were found, including copies of IS3, Tn1000 and a novel mercury resistance transposon, Tn6182. The antibiotic resistance genes were in two locations. Tn6023, containing the aphA1 kanamycin and neomycin resistance gene, is in a partial copy of Tn1/Tn2/Tn3 (blaTEM, ampicillin resistance) in the kfrA gene, and the sul2 sulphonamide resistance gene is in the integrative element GIsul2 in the position of ARI-B islands. The 11.5 kb class II transposon Tn6182 is only distantly related to other class II transposons, with at most 33% identity between the TnpA of Tn6182 and TnpA of other group members. In addition, the inverted repeats are 37 bp rather than 38 bp, and the likely resolution enzyme is a tyrosine recombinase (TnpI). Re-annotation of GIsul2 revealed genes predicted to confer resistance to arsenate and arsenite, but resistance was not detected. The location of GIsul2 confirms it as the progenitor of the ARI-B configurations seen in many IncC plasmids isolated more recently. However, GIsul2 has integrated at the same site in type 1 and type 2 IncC plasmids, indicating that it targets this site. Analysis of the distribution of GIsul2 revealed that it in addition to its chromosomal integration site at the 3′-end of the guaA gene, it has also integrated into other plasmids, increasing its mobility.

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http://hdl.handle.net/10453/123978