Comparison of transcriptome profiles by Fusarium oxysporum inoculation between Fusarium yellows resistant and susceptible lines in Brassica rapa L.

Miyaji, N; Shimizu, M; Miyazaki, J; Osabe, K; Sato, M; Ebe, Y; Takada, S; Kaji, M; Dennis, ES; Fujimoto, R; Okazaki, K

Comparison of transcriptome profiles by Fusarium oxysporum inoculation between Fusarium yellows resistant and susceptible lines in Brassica rapa L.

Miyaji, N Shimizu, M Miyazaki, J Osabe, K Sato, M Ebe, Y Takada, S Kaji, M Dennis, ES

Fujimoto, R Okazaki, K

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Publication Type:: Journal Article
Citation:: Plant Cell Reports, 2017, 36 (12), pp. 1841 - 1854
Issue Date:: 2017-12-01

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Field	Value	Language
dc.contributor.author	Miyaji, N	en_US
dc.contributor.author	Shimizu, M	en_US
dc.contributor.author	Miyazaki, J	en_US
dc.contributor.author	Osabe, K	en_US
dc.contributor.author	Sato, M	en_US
dc.contributor.author	Ebe, Y	en_US
dc.contributor.author	Takada, S	en_US
dc.contributor.author	Kaji, M	en_US
dc.contributor.author	Dennis, ES https://orcid.org/0000-0002-7850-591X	en_US
dc.contributor.author	Fujimoto, R	en_US
dc.contributor.author	Okazaki, K	en_US
dc.date.available	2017-08-07	en_US
dc.date.issued	2017-12-01	en_US
dc.identifier.citation	Plant Cell Reports, 2017, 36 (12), pp. 1841 - 1854	en_US
dc.identifier.issn	0721-7714	en_US
dc.identifier.uri	http://hdl.handle.net/10453/124012
dc.description.abstract	© 2017, Springer-Verlag GmbH Germany. Key message: Resistant and susceptible lines in Brassica rapahave different immune responses against Fusarium oxysporum inoculation. Abstract: Fusarium yellows caused by Fusarium oxysporum f. sp. conglutinans (Foc) is an important disease of Brassicaceae; however, the mechanism of how host plants respond to Foc is still unknown. By comparing with and without Foc inoculation in both resistant and susceptible lines of Chinese cabbage (Brassica rapa var. pekinensis), we identified differentially expressed genes (DEGs) between the bulked inoculated (6, 12, 24, and 72 h after inoculation (HAI)) and non-inoculated samples. Most of the DEGs were up-regulated by Foc inoculation. Quantitative real-time RT-PCR showed that most up-regulated genes increased their expression levels from 24 HAI. An independent transcriptome analysis at 24 and 72 HAI was performed in resistant and susceptible lines. GO analysis using up-regulated genes at 24 HAI indicated that Foc inoculation activated systemic acquired resistance (SAR) in resistant lines and tryptophan biosynthetic process and responses to chitin and ethylene in susceptible lines. By contrast, GO analysis using up-regulated genes at 72 HAI showed the overrepresentation of some categories for the defense response in susceptible lines but not in the resistant lines. We also compared DEGs between B. rapa and Arabidopsis thaliana after F. oxysporum inoculation at the same time point, and identified genes related to defense response that were up-regulated in the resistant lines of Chinese cabbage and A. thaliana. Particular genes that changed expression levels overlapped between the two species, suggesting that they are candidates for genes involved in the resistance mechanisms against F. oxysporum.	en_US
dc.relation.ispartof	Plant Cell Reports	en_US
dc.relation.isbasedon	10.1007/s00299-017-2198-9	en_US
dc.subject.classification	Plant Biology & Botany	en_US
dc.subject.mesh	Fusarium	en_US
dc.subject.mesh	Brassica	en_US
dc.subject.mesh	Brassica rapa	en_US
dc.subject.mesh	Ethylenes	en_US
dc.subject.mesh	Chitin	en_US
dc.subject.mesh	Gene Expression Profiling	en_US
dc.subject.mesh	Gene Expression Regulation, Plant	en_US
dc.subject.mesh	Transcriptome	en_US
dc.title	Comparison of transcriptome profiles by Fusarium oxysporum inoculation between Fusarium yellows resistant and susceptible lines in Brassica rapa L.	en_US
dc.type	Journal Article
utslib.citation.volume	12	en_US
utslib.citation.volume	36	en_US
utslib.for	0607 Plant Biology	en_US
utslib.for	0703 Crop and Pasture Production	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access
pubs.issue	12	en_US
pubs.publication-status	Published	en_US
pubs.volume	36	en_US

Abstract:

© 2017, Springer-Verlag GmbH Germany. Key message: Resistant and susceptible lines in Brassica rapahave different immune responses against Fusarium oxysporum inoculation. Abstract: Fusarium yellows caused by Fusarium oxysporum f. sp. conglutinans (Foc) is an important disease of Brassicaceae; however, the mechanism of how host plants respond to Foc is still unknown. By comparing with and without Foc inoculation in both resistant and susceptible lines of Chinese cabbage (Brassica rapa var. pekinensis), we identified differentially expressed genes (DEGs) between the bulked inoculated (6, 12, 24, and 72 h after inoculation (HAI)) and non-inoculated samples. Most of the DEGs were up-regulated by Foc inoculation. Quantitative real-time RT-PCR showed that most up-regulated genes increased their expression levels from 24 HAI. An independent transcriptome analysis at 24 and 72 HAI was performed in resistant and susceptible lines. GO analysis using up-regulated genes at 24 HAI indicated that Foc inoculation activated systemic acquired resistance (SAR) in resistant lines and tryptophan biosynthetic process and responses to chitin and ethylene in susceptible lines. By contrast, GO analysis using up-regulated genes at 72 HAI showed the overrepresentation of some categories for the defense response in susceptible lines but not in the resistant lines. We also compared DEGs between B. rapa and Arabidopsis thaliana after F. oxysporum inoculation at the same time point, and identified genes related to defense response that were up-regulated in the resistant lines of Chinese cabbage and A. thaliana. Particular genes that changed expression levels overlapped between the two species, suggesting that they are candidates for genes involved in the resistance mechanisms against F. oxysporum.

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http://hdl.handle.net/10453/124012