Effect of carbon limitation on photosynthetic electron transport in Nannochloropsis oculata

Zavřel, T; Szabó, M; Tamburic, B; Evenhuis, C; Kuzhiumparambil, U; Literáková, P; Larkum, AWD; Raven, JA; Červený, J; Ralph, PJ

Effect of carbon limitation on photosynthetic electron transport in Nannochloropsis oculata

Zavřel, T Szabó, M

Tamburic, B

Evenhuis, C

Kuzhiumparambil, U

Literáková, P Larkum, AWD

Raven, JA

Červený, J

Ralph, PJ

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Publication Type:: Journal Article
Citation:: Journal of Photochemistry and Photobiology B: Biology, 2018, 181 pp. 31 - 43
Issue Date:: 2018-04-01

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Field	Value	Language
dc.contributor.author	Zavřel, T	en_US
dc.contributor.author	Szabó, M https://orcid.org/0000-0001-6235-2894	en_US
dc.contributor.author	Tamburic, B https://orcid.org/0000-0001-5720-9380	en_US
dc.contributor.author	Evenhuis, C https://orcid.org/0000-0001-9903-7716	en_US
dc.contributor.author	Kuzhiumparambil, U https://orcid.org/0000-0003-0582-6779	en_US
dc.contributor.author	Literáková, P	en_US
dc.contributor.author	Larkum, AWD https://orcid.org/0000-0002-0420-134X	en_US
dc.contributor.author	Raven, JA https://orcid.org/0000-0002-2789-3297	en_US
dc.contributor.author	Červený, J https://orcid.org/0000-0002-5046-3105	en_US
dc.contributor.author	Ralph, PJ https://orcid.org/0000-0002-3103-7346	en_US
dc.date.available	2018-02-17	en_US
dc.date.issued	2018-04-01	en_US
dc.identifier.citation	Journal of Photochemistry and Photobiology B: Biology, 2018, 181 pp. 31 - 43	en_US
dc.identifier.issn	1011-1344	en_US
dc.identifier.uri	http://hdl.handle.net/10453/124052
dc.description.abstract	© 2018 Elsevier B.V. This study describes the impacts of inorganic carbon limitation on the photosynthetic efficiency and operation of photosynthetic electron transport pathways in the biofuel-candidate microalga Nannochloropsis oculata. Using a combination of highly-controlled cultivation setup (photobioreactor), variable chlorophyll a fluorescence and transient spectroscopy methods (electrochromic shift (ECS) and P700 redox kinetics), we showed that net photosynthesis and effective quantum yield of Photosystem II (PSII) decreased in N. oculata under carbon limitation. This was accompanied by a transient increase in total proton motive force and energy-dependent non-photochemical quenching as well as slightly elevated respiration. On the other hand, under carbon limitation the rapid increase in proton motive force (PMF, estimated from the total ECS signal) was also accompanied by reduced conductivity of ATP synthase to protons (estimated from the rate of ECS decay in dark after actinic illumination). This indicates that the slow operation of ATP synthase results in the transient build-up of PMF, which leads to the activation of fast energy dissipation mechanisms such as energy-dependent non-photochemical quenching. N. oculata also increased content of lipids under carbon limitation, which compensated for reduced NAPDH consumption during decreased CO2 fixation. The integrated knowledge of the underlying energetic regulation of photosynthetic processes attained with a combination of biophysical methods may be used to identify photo-physiological signatures of the onset of carbon limitation in microalgal cultivation systems, as well as to potentially identify microalgal strains that can better acclimate to carbon limitation.	en_US
dc.relation.ispartof	Journal of Photochemistry and Photobiology B: Biology	en_US
dc.relation.isbasedon	10.1016/j.jphotobiol.2018.02.020	en_US
dc.subject.classification	Biophysics	en_US
dc.subject.mesh	Thylakoids	en_US
dc.subject.mesh	Carbon Dioxide	en_US
dc.subject.mesh	Protons	en_US
dc.subject.mesh	Carbon	en_US
dc.subject.mesh	Photosystem II Protein Complex	en_US
dc.subject.mesh	Fatty Acids	en_US
dc.subject.mesh	Adenosine Triphosphate	en_US
dc.subject.mesh	Photosynthesis	en_US
dc.subject.mesh	Electron Transport	en_US
dc.subject.mesh	Light	en_US
dc.subject.mesh	Microalgae	en_US
dc.title	Effect of carbon limitation on photosynthetic electron transport in Nannochloropsis oculata	en_US
dc.type	Journal Article
utslib.citation.volume	181	en_US
utslib.for	0601 Biochemistry and Cell Biology	en_US
utslib.for	0299 Other Physical Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	closed_access
pubs.publication-status	Published	en_US
pubs.volume	181	en_US

Abstract:

© 2018 Elsevier B.V. This study describes the impacts of inorganic carbon limitation on the photosynthetic efficiency and operation of photosynthetic electron transport pathways in the biofuel-candidate microalga Nannochloropsis oculata. Using a combination of highly-controlled cultivation setup (photobioreactor), variable chlorophyll a fluorescence and transient spectroscopy methods (electrochromic shift (ECS) and P700 redox kinetics), we showed that net photosynthesis and effective quantum yield of Photosystem II (PSII) decreased in N. oculata under carbon limitation. This was accompanied by a transient increase in total proton motive force and energy-dependent non-photochemical quenching as well as slightly elevated respiration. On the other hand, under carbon limitation the rapid increase in proton motive force (PMF, estimated from the total ECS signal) was also accompanied by reduced conductivity of ATP synthase to protons (estimated from the rate of ECS decay in dark after actinic illumination). This indicates that the slow operation of ATP synthase results in the transient build-up of PMF, which leads to the activation of fast energy dissipation mechanisms such as energy-dependent non-photochemical quenching. N. oculata also increased content of lipids under carbon limitation, which compensated for reduced NAPDH consumption during decreased CO2 fixation. The integrated knowledge of the underlying energetic regulation of photosynthetic processes attained with a combination of biophysical methods may be used to identify photo-physiological signatures of the onset of carbon limitation in microalgal cultivation systems, as well as to potentially identify microalgal strains that can better acclimate to carbon limitation.

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http://hdl.handle.net/10453/124052