Systemic antibody response to Chlamydia Trachomatis infection in patients either infected or reinfected with different Chlamydia serovars

Gupta, VK; Waugh, CA; Ziklo, N; Huston, WM; Hocking, JS; Timms, P

Systemic antibody response to Chlamydia Trachomatis infection in patients either infected or reinfected with different Chlamydia serovars

Gupta, VK Waugh, CA Ziklo, N Huston, WM

Hocking, JS Timms, P

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Publication Type:: Journal Article
Citation:: Indian Journal of Medical Microbiology, 2017, 35 (3), pp. 394 - 401
Issue Date:: 2017-07-01

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Field	Value	Language
dc.contributor.author	Gupta, VK	en_US
dc.contributor.author	Waugh, CA	en_US
dc.contributor.author	Ziklo, N	en_US
dc.contributor.author	Huston, WM https://orcid.org/0000-0002-0879-1287	en_US
dc.contributor.author	Hocking, JS	en_US
dc.contributor.author	Timms, P	en_US
dc.date.issued	2017-07-01	en_US
dc.identifier.citation	Indian Journal of Medical Microbiology, 2017, 35 (3), pp. 394 - 401	en_US
dc.identifier.issn	0255-0857	en_US
dc.identifier.uri	http://hdl.handle.net/10453/124689
dc.description.abstract	© 2017 Indian Journal of Medical Microbiology \| Published by Wolters Kluwer - Medknow. Introduction: Chlamydia trachomatis is the etiological agent for the most prevalent bacterial sexually transmitted infection in both developed and developing countries. The aim of present study was to characterize the antibody response between two groups of individuals, having either a single C. trachomatis infection and or repeated infections. Material and Methods: Current study consisted of two groups, one with an initial Chlamydia infection and a second with repeated infections. A titre based estimation of specific serum (IgG and IgA) levels using ELISA were performed, which further validated by western blot. In vitro neutralizing ability of each patient's serum against both homologous and heterologous strains was also determined. Results: Individuals infected with one of the C. trachomatis serovars D, E or K exhibited a strong systemic antibody response as characterized by ELISA and western blot. These individuals may have developed at least some level of protection as they only represented single infection. By comparison, individuals infected with serovar D, E or F that exhibited low systemic antibody response often presented repeated C. trachomatis infections, suggesting an association with poor immune response. An in vitro neutralizing level of 60-90% was observed in the human sera against homologous serovar D and two heterologous C. trachomatis serovars E and K, compared to <40% against heterologous serovars F. Conclusion: Individuals infected with serovars D and K showed a potential association between circulating antibody response and re-infection risk. While the patients infected with serovars E showed a disconnection between systemic antibody response and re-infection risk.	en_US
dc.relation.ispartof	Indian Journal of Medical Microbiology	en_US
dc.relation.isbasedon	10.4103/ijmm.IJMM_17_1	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Chlamydia trachomatis	en_US
dc.subject.mesh	Chlamydia Infections	en_US
dc.subject.mesh	Immunoglobulin A	en_US
dc.subject.mesh	Immunoglobulin G	en_US
dc.subject.mesh	Antibodies, Bacterial	en_US
dc.subject.mesh	Blotting, Western	en_US
dc.subject.mesh	Enzyme-Linked Immunosorbent Assay	en_US
dc.subject.mesh	Cohort Studies	en_US
dc.subject.mesh	Antibody Formation	en_US
dc.subject.mesh	Female	en_US
dc.subject.mesh	Antibodies, Neutralizing	en_US
dc.title	Systemic antibody response to Chlamydia Trachomatis infection in patients either infected or reinfected with different Chlamydia serovars	en_US
dc.type	Journal Article
utslib.citation.volume	3	en_US
utslib.citation.volume	35	en_US
utslib.for	1101 Medical Biochemistry and Metabolomics	en_US
utslib.for	11 Medical and Health Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	open_access
pubs.issue	3	en_US
pubs.publication-status	Published	en_US
pubs.volume	35	en_US

Abstract:

© 2017 Indian Journal of Medical Microbiology | Published by Wolters Kluwer - Medknow. Introduction: Chlamydia trachomatis is the etiological agent for the most prevalent bacterial sexually transmitted infection in both developed and developing countries. The aim of present study was to characterize the antibody response between two groups of individuals, having either a single C. trachomatis infection and or repeated infections. Material and Methods: Current study consisted of two groups, one with an initial Chlamydia infection and a second with repeated infections. A titre based estimation of specific serum (IgG and IgA) levels using ELISA were performed, which further validated by western blot. In vitro neutralizing ability of each patient's serum against both homologous and heterologous strains was also determined. Results: Individuals infected with one of the C. trachomatis serovars D, E or K exhibited a strong systemic antibody response as characterized by ELISA and western blot. These individuals may have developed at least some level of protection as they only represented single infection. By comparison, individuals infected with serovar D, E or F that exhibited low systemic antibody response often presented repeated C. trachomatis infections, suggesting an association with poor immune response. An in vitro neutralizing level of 60-90% was observed in the human sera against homologous serovar D and two heterologous C. trachomatis serovars E and K, compared to <40% against heterologous serovars F. Conclusion: Individuals infected with serovars D and K showed a potential association between circulating antibody response and re-infection risk. While the patients infected with serovars E showed a disconnection between systemic antibody response and re-infection risk.

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http://hdl.handle.net/10453/124689