Asymptomatic infections with highly polymorphic Chlamydia suis are ubiquitous in pigs

Li, M; Jelocnik, M; Yang, F; Gong, J; Kaltenboeck, B; Polkinghorne, A; Feng, Z; Pannekoek, Y; Borel, N; Song, C; Jiang, P; Li, J; Zhang, J; Wang, Y; Wang, J; Zhou, X; Wang, C

Asymptomatic infections with highly polymorphic Chlamydia suis are ubiquitous in pigs

Li, M Jelocnik, M

Yang, F Gong, J Kaltenboeck, B Polkinghorne, A Feng, Z Pannekoek, Y Borel, N Song, C Jiang, P Li, J Zhang, J Wang, Y Wang, J Zhou, X Wang, C

Permalink

Publication Type:: Journal Article
Citation:: BMC Veterinary Research, 2017, 13 (1)
Issue Date:: 2017-12-01

Open Access

Copyright Clearance Process

Recently Added
In Progress
Open Access

This item is open access.

Adobe PDF

Download Published VersionAdobe PDF (1.56 MB)

View on publisher's site

View statistics

Full metadata record

Field	Value	Language
dc.contributor.author	Li, M	en_US
dc.contributor.author	Jelocnik, M https://orcid.org/0000-0003-1340-2562	en_US
dc.contributor.author	Yang, F	en_US
dc.contributor.author	Gong, J	en_US
dc.contributor.author	Kaltenboeck, B	en_US
dc.contributor.author	Polkinghorne, A	en_US
dc.contributor.author	Feng, Z	en_US
dc.contributor.author	Pannekoek, Y	en_US
dc.contributor.author	Borel, N	en_US
dc.contributor.author	Song, C	en_US
dc.contributor.author	Jiang, P	en_US
dc.contributor.author	Li, J	en_US
dc.contributor.author	Zhang, J	en_US
dc.contributor.author	Wang, Y	en_US
dc.contributor.author	Wang, J	en_US
dc.contributor.author	Zhou, X	en_US
dc.contributor.author	Wang, C	en_US
dc.date.available	2017-11-22	en_US
dc.date.issued	2017-12-01	en_US
dc.identifier.citation	BMC Veterinary Research, 2017, 13 (1)	en_US
dc.identifier.uri	http://hdl.handle.net/10453/124707
dc.description.abstract	© 2017 The Author(s). Background: Chlamydia suis is an important, globally distributed, highly prevalent and diverse obligate intracellular pathogen infecting pigs. To investigate the prevalence and genetic diversity of C. suis in China, 2,137 nasal, conjunctival, and rectal swabs as well as whole blood and lung samples of pigs were collected in 19 regions from ten provinces of China in this study. Results: We report an overall positivity of 62.4% (1,334/2,137) of C. suis following screening by Chlamydia spp. 23S rRNA-based FRET-PCR and high-resolution melting curve analysis and confirmatory sequencing. For C. suis-positive samples, 33.3 % of whole blood and 62.5% of rectal swabs were found to be positive for the C. suis tetR(C) gene, while 13.3% of whole blood and 87.0% of rectal swabs were positive for the C. suis tet(C) gene. Phylogenetic comparison of partial C. suis ompA gene sequences revealed significant genetic diversity in the C. suis strains. This genetic diversity was confirmed by C. suis-specific multilocus sequence typing (MLST), which identified 26 novel sequence types among 27 examined strains. Tanglegrams based on MLST and ompA sequences provided evidence of C. suis recombination amongst the strains analyzed. Conclusions: Genetically highly diverse C. suis strains are exceedingly prevalent in pigs. As it stands, the potential pathogenic effect of C. suis on pig health and production of C. suis remains unclear and will be the subject of further investigations. Further study is also required to address the transmission of C. suis between pigs and the risk of 'spill-over' and 'spill-back' of infections to wild animals and humans.	en_US
dc.relation.ispartof	BMC Veterinary Research	en_US
dc.relation.isbasedon	10.1186/s12917-017-1295-x	en_US
dc.subject.classification	Veterinary Sciences	en_US
dc.subject.mesh	Rectum	en_US
dc.subject.mesh	Lung	en_US
dc.subject.mesh	Nasal Cavity	en_US
dc.subject.mesh	Conjunctiva	en_US
dc.subject.mesh	Blood	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Swine	en_US
dc.subject.mesh	Chlamydia	en_US
dc.subject.mesh	Chlamydia Infections	en_US
dc.subject.mesh	Swine Diseases	en_US
dc.subject.mesh	RNA, Ribosomal, 23S	en_US
dc.subject.mesh	Fluorescence Resonance Energy Transfer	en_US
dc.subject.mesh	Phylogeny	en_US
dc.subject.mesh	China	en_US
dc.subject.mesh	Genetic Variation	en_US
dc.subject.mesh	Asymptomatic Infections	en_US
dc.subject.mesh	Real-Time Polymerase Chain Reaction	en_US
dc.title	Asymptomatic infections with highly polymorphic Chlamydia suis are ubiquitous in pigs	en_US
dc.type	Journal Article
utslib.citation.volume	1	en_US
utslib.citation.volume	13	en_US
utslib.for	0707 Veterinary Sciences	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	0601 Biochemistry and Cell Biology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	open_access
pubs.issue	1	en_US
pubs.publication-status	Published	en_US
pubs.volume	13	en_US

Abstract:

© 2017 The Author(s). Background: Chlamydia suis is an important, globally distributed, highly prevalent and diverse obligate intracellular pathogen infecting pigs. To investigate the prevalence and genetic diversity of C. suis in China, 2,137 nasal, conjunctival, and rectal swabs as well as whole blood and lung samples of pigs were collected in 19 regions from ten provinces of China in this study. Results: We report an overall positivity of 62.4% (1,334/2,137) of C. suis following screening by Chlamydia spp. 23S rRNA-based FRET-PCR and high-resolution melting curve analysis and confirmatory sequencing. For C. suis-positive samples, 33.3 % of whole blood and 62.5% of rectal swabs were found to be positive for the C. suis tetR(C) gene, while 13.3% of whole blood and 87.0% of rectal swabs were positive for the C. suis tet(C) gene. Phylogenetic comparison of partial C. suis ompA gene sequences revealed significant genetic diversity in the C. suis strains. This genetic diversity was confirmed by C. suis-specific multilocus sequence typing (MLST), which identified 26 novel sequence types among 27 examined strains. Tanglegrams based on MLST and ompA sequences provided evidence of C. suis recombination amongst the strains analyzed. Conclusions: Genetically highly diverse C. suis strains are exceedingly prevalent in pigs. As it stands, the potential pathogenic effect of C. suis on pig health and production of C. suis remains unclear and will be the subject of further investigations. Further study is also required to address the transmission of C. suis between pigs and the risk of 'spill-over' and 'spill-back' of infections to wild animals and humans.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/124707