Safety and immunogenicity of a prototype anti-Chlamydia pecorum recombinant protein vaccine in lambs and pregnant ewes

Desclozeaux, M; Jelocnik, M; Whitting, K; Saifzadeh, S; Bommana, S; Potter, A; Gerdts, V; Timms, P; Polkinghorne, A

Safety and immunogenicity of a prototype anti-Chlamydia pecorum recombinant protein vaccine in lambs and pregnant ewes

Desclozeaux, M Jelocnik, M

Whitting, K Saifzadeh, S Bommana, S Potter, A Gerdts, V Timms, P Polkinghorne, A

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Publication Type:: Journal Article
Citation:: Vaccine, 2017, 35 (27), pp. 3461 - 3465
Issue Date:: 2017-06-14

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Field	Value	Language
dc.contributor.author	Desclozeaux, M	en_US
dc.contributor.author	Jelocnik, M https://orcid.org/0000-0003-1340-2562	en_US
dc.contributor.author	Whitting, K	en_US
dc.contributor.author	Saifzadeh, S	en_US
dc.contributor.author	Bommana, S	en_US
dc.contributor.author	Potter, A	en_US
dc.contributor.author	Gerdts, V	en_US
dc.contributor.author	Timms, P	en_US
dc.contributor.author	Polkinghorne, A	en_US
dc.date.available	2017-03-30	en_US
dc.date.issued	2017-06-14	en_US
dc.identifier.citation	Vaccine, 2017, 35 (27), pp. 3461 - 3465	en_US
dc.identifier.issn	0264-410X	en_US
dc.identifier.uri	http://hdl.handle.net/10453/124711
dc.description.abstract	© 2017 Elsevier Ltd Arthritis and kerato-conjunctivitis caused by Chlamydia pecorum in lambs are difficult to diagnose and treat. We tested the ability of a prototype C. pecorum vaccine (SC-vaccine), comprised of C. pecorum major outer membrane protein (MOMP-G) and polymorphic membrane protein G (PmpG), to trigger a Chlamydia-specific humoral and cell-mediated immune response in lambs and pregnant ewes. Vaccinations with the SC-vaccine (one and two injections) were very well tolerated by all ewes and lambs. Although the overall immune responses of ewes to SC-vaccination was poor, their lambs showed stronger antigen-specific immune response than lambs from control vaccine ewes. SC-vaccination in lambs triggered production of systemic anti-MOMP-G and anti-PmpG IgG antibodies and secretory IgA in the ocular mucosa. Double vaccination caused statistically significant increases in the height and duration of the humoral response. Antigen-specific IFN-γ was produced in the peripheral blood mononuclear cells of vaccinated lambs.	en_US
dc.relation.ispartof	Vaccine	en_US
dc.relation.isbasedon	10.1016/j.vaccine.2017.03.091	en_US
dc.subject.classification	Virology	en_US
dc.subject.mesh	Conjunctiva	en_US
dc.subject.mesh	Leukocytes, Mononuclear	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Sheep	en_US
dc.subject.mesh	Chlamydia	en_US
dc.subject.mesh	Chlamydia Infections	en_US
dc.subject.mesh	Sheep Diseases	en_US
dc.subject.mesh	Immunoglobulin A	en_US
dc.subject.mesh	Immunoglobulin G	en_US
dc.subject.mesh	Vaccines, Synthetic	en_US
dc.subject.mesh	Bacterial Vaccines	en_US
dc.subject.mesh	Antibodies, Bacterial	en_US
dc.subject.mesh	Immunity, Cellular	en_US
dc.subject.mesh	Pregnancy	en_US
dc.subject.mesh	Female	en_US
dc.subject.mesh	Interferon-gamma	en_US
dc.subject.mesh	Immunity, Humoral	en_US
dc.title	Safety and immunogenicity of a prototype anti-Chlamydia pecorum recombinant protein vaccine in lambs and pregnant ewes	en_US
dc.type	Journal Article
utslib.citation.volume	27	en_US
utslib.citation.volume	35	en_US
utslib.for	0601 Biochemistry and Cell Biology	en_US
utslib.for	0701 Agriculture, Land and Farm Management	en_US
utslib.for	1101 Medical Biochemistry and Metabolomics	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	07 Agricultural and Veterinary Sciences	en_US
utslib.for	11 Medical and Health Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access
pubs.issue	27	en_US
pubs.publication-status	Published	en_US
pubs.volume	35	en_US

Abstract:

© 2017 Elsevier Ltd Arthritis and kerato-conjunctivitis caused by Chlamydia pecorum in lambs are difficult to diagnose and treat. We tested the ability of a prototype C. pecorum vaccine (SC-vaccine), comprised of C. pecorum major outer membrane protein (MOMP-G) and polymorphic membrane protein G (PmpG), to trigger a Chlamydia-specific humoral and cell-mediated immune response in lambs and pregnant ewes. Vaccinations with the SC-vaccine (one and two injections) were very well tolerated by all ewes and lambs. Although the overall immune responses of ewes to SC-vaccination was poor, their lambs showed stronger antigen-specific immune response than lambs from control vaccine ewes. SC-vaccination in lambs triggered production of systemic anti-MOMP-G and anti-PmpG IgG antibodies and secretory IgA in the ocular mucosa. Double vaccination caused statistically significant increases in the height and duration of the humoral response. Antigen-specific IFN-γ was produced in the peripheral blood mononuclear cells of vaccinated lambs.

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http://hdl.handle.net/10453/124711