Influence of surface composition on the colloidal stability of ultra-small detonation nanodiamonds in biological media

Bradac, C; Rastogi, ID; Cordina, NM; Garcia-Bennett, A; Brown, LJ

Influence of surface composition on the colloidal stability of ultra-small detonation nanodiamonds in biological media

Bradac, C

Rastogi, ID Cordina, NM Garcia-Bennett, A

Brown, LJ

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Publication Type:: Journal Article
Citation:: Diamond and Related Materials, 2018, 83 pp. 38 - 45
Issue Date:: 2018-03-01

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Field	Value	Language
dc.contributor.author	Bradac, C https://orcid.org/0000-0002-6673-7238	en_US
dc.contributor.author	Rastogi, ID	en_US
dc.contributor.author	Cordina, NM	en_US
dc.contributor.author	Garcia-Bennett, A https://orcid.org/0000-0002-9097-0179	en_US
dc.contributor.author	Brown, LJ https://orcid.org/0000-0001-8592-2431	en_US
dc.date.available	2020-05-25T19:02:19Z
dc.date.issued	2018-03-01	en_US
dc.identifier.citation	Diamond and Related Materials, 2018, 83 pp. 38 - 45	en_US
dc.identifier.issn	0925-9635	en_US
dc.identifier.uri	http://hdl.handle.net/10453/124816
dc.description.abstract	© 2018 Fluorescent nanodiamonds (NDs) are strong contenders as bio-labels for life science imaging, diagnostics and therapeutics. Ultimately, for their use in biomedical applications, their size should ideally be <10 nm. Yet, even more critical for their specificity and efficient uptake in cellular systems, is their resilience to aggregation, which is dictated by their colloidal stability in complex, physiological environments. To this end, we characterize small detonation NDs (~5 nm) by examining their surface chemical profiles and stability in solutions of varying ionic strength and pH. Using dynamic light scattering measurements, we demonstrate that small monodisperse ND particles with chemically homogeneous and negatively charged surface profiles are more stable than positive particles under a broad range of simulated biological environments. We show that the colloidal stability of small clusters of both positive and negative detonation NDs is improved by functionalization with bovine serum albumin. Based on these analyses, we propose and describe strategies for enhancing the overall colloidal stability of detonation NDs and their resilience to aggregation. Our findings provide a practical framework towards the reduction in size of the bio-conjugates employed to probe complex biological systems, and the advancement of bio-imaging techniques with minimal perturbation of the molecular trafficking in cellular and organelle systems.	en_US
dc.relation	http://purl.org/au-research/grants/arc/DE180100810
dc.relation	http://purl.org/au-research/grants/arc/LE180100090
dc.relation.ispartof	Diamond and Related Materials	en_US
dc.relation.isbasedon	10.1016/j.diamond.2018.01.022	en_US
dc.subject.classification	Applied Physics	en_US
dc.title	Influence of surface composition on the colloidal stability of ultra-small detonation nanodiamonds in biological media	en_US
dc.type	Journal Article
utslib.citation.volume	83	en_US
utslib.for	0904 Chemical Engineering	en_US
utslib.for	0912 Materials Engineering	en_US
utslib.for	0910 Manufacturing Engineering	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
utslib.copyright.status	open_access
pubs.publication-status	Published	en_US
pubs.volume	83	en_US

Abstract:

© 2018 Fluorescent nanodiamonds (NDs) are strong contenders as bio-labels for life science imaging, diagnostics and therapeutics. Ultimately, for their use in biomedical applications, their size should ideally be <10 nm. Yet, even more critical for their specificity and efficient uptake in cellular systems, is their resilience to aggregation, which is dictated by their colloidal stability in complex, physiological environments. To this end, we characterize small detonation NDs (~5 nm) by examining their surface chemical profiles and stability in solutions of varying ionic strength and pH. Using dynamic light scattering measurements, we demonstrate that small monodisperse ND particles with chemically homogeneous and negatively charged surface profiles are more stable than positive particles under a broad range of simulated biological environments. We show that the colloidal stability of small clusters of both positive and negative detonation NDs is improved by functionalization with bovine serum albumin. Based on these analyses, we propose and describe strategies for enhancing the overall colloidal stability of detonation NDs and their resilience to aggregation. Our findings provide a practical framework towards the reduction in size of the bio-conjugates employed to probe complex biological systems, and the advancement of bio-imaging techniques with minimal perturbation of the molecular trafficking in cellular and organelle systems.

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http://hdl.handle.net/10453/124816