Functionalisation of Ti6Al4V and hydroxyapatite surfaces with combined peptides based on KKLPDA and EEEEEEEE peptides

Rodriguez, GM; Bowen, J; Grossin, D; Ben-Nissan, B; Stamboulis, A

Functionalisation of Ti6Al4V and hydroxyapatite surfaces with combined peptides based on KKLPDA and EEEEEEEE peptides

Rodriguez, GM Bowen, J Grossin, D Ben-Nissan, B Stamboulis, A

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Publication Type:: Journal Article
Citation:: Colloids and Surfaces B: Biointerfaces, 2017, 160 pp. 154 - 160
Issue Date:: 2017-12-01

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Field	Value	Language
dc.contributor.author	Rodriguez, GM	en_US
dc.contributor.author	Bowen, J	en_US
dc.contributor.author	Grossin, D	en_US
dc.contributor.author	Ben-Nissan, B	en_US
dc.contributor.author	Stamboulis, A https://orcid.org/0000-0002-8366-590X	en_US
dc.date.available	2020-05-25T19:23:25Z
dc.date.issued	2017-12-01	en_US
dc.identifier.citation	Colloids and Surfaces B: Biointerfaces, 2017, 160 pp. 154 - 160	en_US
dc.identifier.issn	0927-7765	en_US
dc.identifier.uri	http://hdl.handle.net/10453/124871
dc.description.abstract	© 2017 The Authors Surface modifications are usually performed on titanium alloys to improve osteo-integration and surface bioactivity. Modifications such as alkaline and acid etching, or coating with bioactive materials such as hydroxyapatite, have previously been demonstrated. The aim of this work is to develop a peptide with combined titanium oxide and hydroxyapatite binders in order to achieve a biomimetic hydroxyapatite coating on titanium surfaces. The technology would also be applicable for the functionalisation of titanium and hydroxyapatite surfaces for selective protein adsorption, conjugation of antimicrobial peptides, and adsorption of specialised drugs for drug delivery. In this work, functionalisation of Ti6Al4V and hydroxyapatite surfaces was achieved using combined titanium-hydroxyapatite (Ti-Hap) peptides based on titanium peptide binder (KKLPDA) and hydroxyapatite peptide binder (EEEEEEEE). Homogeneous peptide coatings on Ti6Al4V surfaces were obtained after surface chemical treatments with a 30 wt% aqueous solution of H2O2 for 24 and 48 h. The treated titanium surfaces presented an average roughness of Sa = 197 nm (24 h) and Sa = 128 nm (48 h); an untreated mirror polished sample exhibited an Sa of 13 nm. The advancing water contact angle of the titanium oxide layer after 1 h of exposure to 30 wt% aqueous solution of H2O2 was around 65°, decreasing gradually with time until it reached 35° after a 48 h exposure, suggesting that the surface hydrophilicity increased over etching time. The presence of a lysine (L) amino acid in the sequence of the titanium binder resulted in fluorescence intensity roughly 16% higher compared with the arginine (R) amino acid analogue and therefore the lysine containing titanium peptide binder was used in this work. The Ti-Hap peptide KKLPDAEEEEEEEE (Ti-Hap1) was not adsorbed by the treated Ti6Al4V surfaces and therefore was modified. The modifications involved the inclusion of a glycine spacer between the binding terminals (Ti-Hap2) and the addition of a second titanium binder (KKLPDA) (Ti-Hap3 and Ti-Hap4). The combined Ti-Hap peptide which exhibited the strongest intensity after the titanium surface dip coating was KKLPDAKKLPDAEEEEEEEE (Ti-HAp4). On the other hand, hydroxyapatite surfaces, exhibiting an average roughness of Sa = 1.42 μm, showed a higher fluorescence for peptides with a higher negative net charge.	en_US
dc.relation.ispartof	Colloids and Surfaces B: Biointerfaces	en_US
dc.relation.isbasedon	10.1016/j.colsurfb.2017.09.022	en_US
dc.subject.classification	Chemical Physics	en_US
dc.subject.mesh	Durapatite	en_US
dc.subject.mesh	Titanium	en_US
dc.subject.mesh	Alloys	en_US
dc.subject.mesh	Peptides	en_US
dc.subject.mesh	Coated Materials, Biocompatible	en_US
dc.subject.mesh	Microscopy, Electron, Scanning	en_US
dc.subject.mesh	Microscopy, Fluorescence	en_US
dc.subject.mesh	Interferometry	en_US
dc.subject.mesh	Amino Acid Sequence	en_US
dc.subject.mesh	Adsorption	en_US
dc.subject.mesh	Surface Properties	en_US
dc.title	Functionalisation of Ti6Al4V and hydroxyapatite surfaces with combined peptides based on KKLPDA and EEEEEEEE peptides	en_US
dc.type	Journal Article
utslib.citation.volume	160	en_US
utslib.for	0306 Physical Chemistry (incl. Structural)	en_US
utslib.for	0903 Biomedical Engineering	en_US
utslib.for	0904 Chemical Engineering	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
utslib.copyright.status	open_access
pubs.publication-status	Published	en_US
pubs.volume	160	en_US

Abstract:

© 2017 The Authors Surface modifications are usually performed on titanium alloys to improve osteo-integration and surface bioactivity. Modifications such as alkaline and acid etching, or coating with bioactive materials such as hydroxyapatite, have previously been demonstrated. The aim of this work is to develop a peptide with combined titanium oxide and hydroxyapatite binders in order to achieve a biomimetic hydroxyapatite coating on titanium surfaces. The technology would also be applicable for the functionalisation of titanium and hydroxyapatite surfaces for selective protein adsorption, conjugation of antimicrobial peptides, and adsorption of specialised drugs for drug delivery. In this work, functionalisation of Ti6Al4V and hydroxyapatite surfaces was achieved using combined titanium-hydroxyapatite (Ti-Hap) peptides based on titanium peptide binder (KKLPDA) and hydroxyapatite peptide binder (EEEEEEEE). Homogeneous peptide coatings on Ti6Al4V surfaces were obtained after surface chemical treatments with a 30 wt% aqueous solution of H2O2 for 24 and 48 h. The treated titanium surfaces presented an average roughness of Sa = 197 nm (24 h) and Sa = 128 nm (48 h); an untreated mirror polished sample exhibited an Sa of 13 nm. The advancing water contact angle of the titanium oxide layer after 1 h of exposure to 30 wt% aqueous solution of H2O2 was around 65°, decreasing gradually with time until it reached 35° after a 48 h exposure, suggesting that the surface hydrophilicity increased over etching time. The presence of a lysine (L) amino acid in the sequence of the titanium binder resulted in fluorescence intensity roughly 16% higher compared with the arginine (R) amino acid analogue and therefore the lysine containing titanium peptide binder was used in this work. The Ti-Hap peptide KKLPDAEEEEEEEE (Ti-Hap1) was not adsorbed by the treated Ti6Al4V surfaces and therefore was modified. The modifications involved the inclusion of a glycine spacer between the binding terminals (Ti-Hap2) and the addition of a second titanium binder (KKLPDA) (Ti-Hap3 and Ti-Hap4). The combined Ti-Hap peptide which exhibited the strongest intensity after the titanium surface dip coating was KKLPDAKKLPDAEEEEEEEE (Ti-HAp4). On the other hand, hydroxyapatite surfaces, exhibiting an average roughness of Sa = 1.42 μm, showed a higher fluorescence for peptides with a higher negative net charge.

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http://hdl.handle.net/10453/124871