Patterns of gene expression in developing embryos of Arabidopsis hybrids

Alonso-Peral, MM; Trigueros, M; Sherman, B; Ying, H; Taylor, JM; Peacock, WJ; Dennis, ES

Patterns of gene expression in developing embryos of Arabidopsis hybrids

Alonso-Peral, MM Trigueros, M Sherman, B Ying, H Taylor, JM Peacock, WJ

Dennis, ES

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Publication Type:: Journal Article
Citation:: Plant Journal, 2017, 89 (5), pp. 927 - 939
Issue Date:: 2017-03-01

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Field	Value	Language
dc.contributor.author	Alonso-Peral, MM	en_US
dc.contributor.author	Trigueros, M	en_US
dc.contributor.author	Sherman, B	en_US
dc.contributor.author	Ying, H	en_US
dc.contributor.author	Taylor, JM	en_US
dc.contributor.author	Peacock, WJ https://orcid.org/0000-0001-7314-9074	en_US
dc.contributor.author	Dennis, ES https://orcid.org/0000-0002-7850-591X	en_US
dc.date.available	2016-11-18	en_US
dc.date.issued	2017-03-01	en_US
dc.identifier.citation	Plant Journal, 2017, 89 (5), pp. 927 - 939	en_US
dc.identifier.issn	0960-7412	en_US
dc.identifier.uri	http://hdl.handle.net/10453/124937
dc.description.abstract	© 2016 The Authors The Plant Journal © 2016 John Wiley & Sons Ltd Hybrids between the Arabidopsis ecotypes C24 and Ler have high levels of hybrid vigour, or heterosis, in both biomass and seed yield. Heterosis can be detected throughout the development of the plant and in different tissues. We examined developing embryos and seeds of C24/Ler reciprocal hybrids with the aim of detecting the earliest time at which heterotic gene activity occurs. In the transcriptomes of 4-dap (days after pollination; dermatogen to globular) and 6-dap (heart) embryos from both parents and hybrids, 95% of expressed genes were at the mid parent value (MPV) and 95% of the genes with single nucleotide polymorphisms between C24 and Ler retained the same relative allelic expression levels in the hybrids as existed in the parents. This included loci that had equivalent levels of transcription in the two parents, together with loci which had different levels of expression in the parents. Amongst the genes which did not have MPV expression levels in the hybrids (non-additively expressed genes), approximately 40 in the globular embryo stage and 89 in the heart embryo stage had altered levels of transcription in both reciprocal hybrids; these genes could contribute to the heterotic phenotype of the hybrid embryo. Many of the non-additively expressed genes had expression levels that were shifted towards maternal levels of transcription, and these differed in the reciprocal hybrids. Allelic expression analysis indicated that most genes with altered allelic contributions in the hybrids had an increase in the expression level of the hybrid's maternal allele. Consistent with the maternal pattern of gene expression, embryo and seed also show maternally influenced phenotypes.	en_US
dc.relation.ispartof	Plant Journal	en_US
dc.relation.isbasedon	10.1111/tpj.13432	en_US
dc.subject.classification	Plant Biology & Botany	en_US
dc.subject.mesh	Arabidopsis	en_US
dc.subject.mesh	Seeds	en_US
dc.subject.mesh	Arabidopsis Proteins	en_US
dc.subject.mesh	Gene Expression Regulation, Plant	en_US
dc.subject.mesh	Transcriptome	en_US
dc.title	Patterns of gene expression in developing embryos of Arabidopsis hybrids	en_US
dc.type	Journal Article
utslib.citation.volume	5	en_US
utslib.citation.volume	89	en_US
utslib.for	0607 Plant Biology	en_US
utslib.for	0601 Biochemistry and Cell Biology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access
pubs.issue	5	en_US
pubs.publication-status	Published	en_US
pubs.volume	89	en_US

Abstract:

© 2016 The Authors The Plant Journal © 2016 John Wiley & Sons Ltd Hybrids between the Arabidopsis ecotypes C24 and Ler have high levels of hybrid vigour, or heterosis, in both biomass and seed yield. Heterosis can be detected throughout the development of the plant and in different tissues. We examined developing embryos and seeds of C24/Ler reciprocal hybrids with the aim of detecting the earliest time at which heterotic gene activity occurs. In the transcriptomes of 4-dap (days after pollination; dermatogen to globular) and 6-dap (heart) embryos from both parents and hybrids, 95% of expressed genes were at the mid parent value (MPV) and 95% of the genes with single nucleotide polymorphisms between C24 and Ler retained the same relative allelic expression levels in the hybrids as existed in the parents. This included loci that had equivalent levels of transcription in the two parents, together with loci which had different levels of expression in the parents. Amongst the genes which did not have MPV expression levels in the hybrids (non-additively expressed genes), approximately 40 in the globular embryo stage and 89 in the heart embryo stage had altered levels of transcription in both reciprocal hybrids; these genes could contribute to the heterotic phenotype of the hybrid embryo. Many of the non-additively expressed genes had expression levels that were shifted towards maternal levels of transcription, and these differed in the reciprocal hybrids. Allelic expression analysis indicated that most genes with altered allelic contributions in the hybrids had an increase in the expression level of the hybrid's maternal allele. Consistent with the maternal pattern of gene expression, embryo and seed also show maternally influenced phenotypes.

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http://hdl.handle.net/10453/124937