Bispecific Antibody-Functionalized Upconversion Nanoprobe

He, H; Howard, CB; Chen, Y; Wen, S; Lin, G; Zhou, J; Thurecht, KJ; Jin, D

Bispecific Antibody-Functionalized Upconversion Nanoprobe

He, H

Howard, CB Chen, Y

Wen, S

Lin, G

Zhou, J

Thurecht, KJ Jin, D

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Publication Type:: Journal Article
Citation:: Analytical Chemistry, 2018, 90 (5), pp. 3024 - 3029
Issue Date:: 2018-03-06

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Field	Value	Language
dc.contributor.author	He, H https://orcid.org/0000-0003-3878-3349	en_US
dc.contributor.author	Howard, CB	en_US
dc.contributor.author	Chen, Y https://orcid.org/0000-0002-7886-7144	en_US
dc.contributor.author	Wen, S https://orcid.org/0000-0002-4670-4658	en_US
dc.contributor.author	Lin, G https://orcid.org/0000-0001-9880-8478	en_US
dc.contributor.author	Zhou, J https://orcid.org/0000-0002-0605-5745	en_US
dc.contributor.author	Thurecht, KJ	en_US
dc.contributor.author	Jin, D https://orcid.org/0000-0003-1046-2666	en_US
dc.date.available	2020-05-25T19:11:25Z
dc.date.issued	2018-03-06	en_US
dc.identifier.citation	Analytical Chemistry, 2018, 90 (5), pp. 3024 - 3029	en_US
dc.identifier.issn	0003-2700	en_US
dc.identifier.uri	http://hdl.handle.net/10453/125025
dc.description.abstract	© 2018 American Chemical Society. Upconversion nanoparticles (UCNPs) are new optical probes for biological applications. For specific biomolecular recognition to be realized for diagnosis and imaging, the key lies in developing a stable and easy-to-use bioconjugation method for antibody modification. Current methods are not yet satisfactory regarding conjugation time, stability, and binding efficiency. Here, we report a facile and high-yield approach based on a bispecific antibody (BsAb) free of chemical reaction steps. One end of the BsAb is designed to recognize methoxy polyethylene glycol-coated UCNPs, and the other end of the BsAb is designed to recognize the cancer antigen biomarker. Through simple vortexing, BsAb-UCNP nanoprobes form within 30 min and show higher (up to 54%) association to the target than that of the traditional UCNP nanoprobes in the ELISA-like assay. We further demonstrate its successful binding to the cancer cells with high efficiency and specificity for background-free fluorescence imaging under near-infrared excitation. This method suggests a general approach broadly suitable for functionalizing a range of nanoparticles to specifically target biomolecules.	en_US
dc.relation	http://purl.org/au-research/grants/arc/IH150100028
dc.relation.ispartof	Analytical Chemistry	en_US
dc.relation.isbasedon	10.1021/acs.analchem.7b05341	en_US
dc.subject.classification	Analytical Chemistry	en_US
dc.subject.mesh	Cell Line, Tumor	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Polyethylene Glycols	en_US
dc.subject.mesh	Receptor, EphA2	en_US
dc.subject.mesh	Antibodies, Bispecific	en_US
dc.subject.mesh	Immunoconjugates	en_US
dc.subject.mesh	Microscopy, Confocal	en_US
dc.subject.mesh	Light	en_US
dc.subject.mesh	Fluorescence	en_US
dc.subject.mesh	Nanoparticles	en_US
dc.title	Bispecific Antibody-Functionalized Upconversion Nanoprobe	en_US
dc.type	Journal Article
utslib.citation.volume	5	en_US
utslib.citation.volume	90	en_US
utslib.for	0301 Analytical Chemistry	en_US
utslib.for	0904 Chemical Engineering	en_US
utslib.for	0399 Other Chemical Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
pubs.organisational-group	/University of Technology Sydney/Students
utslib.copyright.status	open_access
pubs.issue	5	en_US
pubs.publication-status	Published	en_US
pubs.volume	90	en_US

Abstract:

© 2018 American Chemical Society. Upconversion nanoparticles (UCNPs) are new optical probes for biological applications. For specific biomolecular recognition to be realized for diagnosis and imaging, the key lies in developing a stable and easy-to-use bioconjugation method for antibody modification. Current methods are not yet satisfactory regarding conjugation time, stability, and binding efficiency. Here, we report a facile and high-yield approach based on a bispecific antibody (BsAb) free of chemical reaction steps. One end of the BsAb is designed to recognize methoxy polyethylene glycol-coated UCNPs, and the other end of the BsAb is designed to recognize the cancer antigen biomarker. Through simple vortexing, BsAb-UCNP nanoprobes form within 30 min and show higher (up to 54%) association to the target than that of the traditional UCNP nanoprobes in the ELISA-like assay. We further demonstrate its successful binding to the cancer cells with high efficiency and specificity for background-free fluorescence imaging under near-infrared excitation. This method suggests a general approach broadly suitable for functionalizing a range of nanoparticles to specifically target biomolecules.

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http://hdl.handle.net/10453/125025