Mechanism of photodamage of the oxygen evolving Mn Cluster of Photosystem II by excessive light energy

Zavafer, A; Koinuma, W; Chow, WS; Cheah, MH; Mino, H

Mechanism of photodamage of the oxygen evolving Mn Cluster of Photosystem II by excessive light energy

Zavafer, A

Koinuma, W Chow, WS Cheah, MH Mino, H

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Publication Type:: Journal Article
Citation:: Scientific Reports, 2017, 7 (1)
Issue Date:: 2017-12-01

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Field	Value	Language
dc.contributor.author	Zavafer, A https://orcid.org/0000-0002-8905-1618	en_US
dc.contributor.author	Koinuma, W	en_US
dc.contributor.author	Chow, WS	en_US
dc.contributor.author	Cheah, MH	en_US
dc.contributor.author	Mino, H	en_US
dc.date.available	2017-06-30	en_US
dc.date.issued	2017-12-01	en_US
dc.identifier.citation	Scientific Reports, 2017, 7 (1)	en_US
dc.identifier.uri	http://hdl.handle.net/10453/125140
dc.description.abstract	© 2017 The Author(s). Photodamage to Photosystem II (PSII) has been attributed either to excessive excitation of photosynthetic pigments or by direct of light absorption by Mn4CaO5 cluster. Here we investigated the time course of PSII photodamage and release of Mn in PSII-enriched membranes under high light illumination at 460 nm and 660 nm. We found that the loss of PSII activity, assayed by chlorophyll fluorescence, is faster than release of Mn from the Mn4CaO5 cluster, assayed by EPR. Loss of PSII activity and Mn release was slower during illumination in the presence of exogenous electron acceptors. Recovery of PSII activity was observed, after 30 min of addition of electron donor post illumination. The same behavior was observed under 460 and 660 nm illumination, suggesting stronger correlation between excessive excitation and photodamage compared to direct light absorption by the cluster. A unified model of PSII photodamage that takes into account present and previous literature reports is presented.	en_US
dc.relation.ispartof	Scientific Reports	en_US
dc.relation.isbasedon	10.1038/s41598-017-07671-1	en_US
dc.subject.mesh	Intracellular Membranes	en_US
dc.subject.mesh	Spinacia oleracea	en_US
dc.subject.mesh	Plant Leaves	en_US
dc.subject.mesh	Oxygen	en_US
dc.subject.mesh	Manganese	en_US
dc.subject.mesh	Chlorophyll	en_US
dc.subject.mesh	Light-Harvesting Protein Complexes	en_US
dc.subject.mesh	Photosystem II Protein Complex	en_US
dc.subject.mesh	Electron Spin Resonance Spectroscopy	en_US
dc.subject.mesh	Photosynthesis	en_US
dc.subject.mesh	Kinetics	en_US
dc.subject.mesh	Electrons	en_US
dc.subject.mesh	Light	en_US
dc.subject.mesh	Fluorescence	en_US
dc.title	Mechanism of photodamage of the oxygen evolving Mn Cluster of Photosystem II by excessive light energy	en_US
dc.type	Journal Article
utslib.citation.volume	1	en_US
utslib.citation.volume	7	en_US
utslib.for	0699 Other Biological Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	open_access
pubs.issue	1	en_US
pubs.publication-status	Published	en_US
pubs.volume	7	en_US

Abstract:

© 2017 The Author(s). Photodamage to Photosystem II (PSII) has been attributed either to excessive excitation of photosynthetic pigments or by direct of light absorption by Mn4CaO5 cluster. Here we investigated the time course of PSII photodamage and release of Mn in PSII-enriched membranes under high light illumination at 460 nm and 660 nm. We found that the loss of PSII activity, assayed by chlorophyll fluorescence, is faster than release of Mn from the Mn4CaO5 cluster, assayed by EPR. Loss of PSII activity and Mn release was slower during illumination in the presence of exogenous electron acceptors. Recovery of PSII activity was observed, after 30 min of addition of electron donor post illumination. The same behavior was observed under 460 and 660 nm illumination, suggesting stronger correlation between excessive excitation and photodamage compared to direct light absorption by the cluster. A unified model of PSII photodamage that takes into account present and previous literature reports is presented.

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http://hdl.handle.net/10453/125140