Microfluidic high performance liquid chromatography-chip hyphenation to inductively coupled plasma–mass spectrometry

Bishop, DP; Blanes, L; Wilson, AB; Wilbanks, T; Killeen, K; Grimm, R; Wenzel, R; Major, D; Macka, M; Clarke, D; Schmid, R; Cole, N; Doble, PA

Microfluidic high performance liquid chromatography-chip hyphenation to inductively coupled plasma–mass spectrometry

Bishop, DP

Blanes, L Wilson, AB Wilbanks, T Killeen, K Grimm, R Wenzel, R

Major, D Macka, M Clarke, D Schmid, R Cole, N

Doble, PA

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Publication Type:: Journal Article
Citation:: Journal of Chromatography A, 2017, 1497 pp. 64 - 69
Issue Date:: 2017-05-12

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Field	Value	Language
dc.contributor.author	Bishop, DP https://orcid.org/0000-0002-6533-4410	en_US
dc.contributor.author	Blanes, L	en_US
dc.contributor.author	Wilson, AB	en_US
dc.contributor.author	Wilbanks, T	en_US
dc.contributor.author	Killeen, K	en_US
dc.contributor.author	Grimm, R	en_US
dc.contributor.author	Wenzel, R https://orcid.org/0000-0002-4395-0239	en_US
dc.contributor.author	Major, D	en_US
dc.contributor.author	Macka, M	en_US
dc.contributor.author	Clarke, D	en_US
dc.contributor.author	Schmid, R	en_US
dc.contributor.author	Cole, N https://orcid.org/0000-0002-9866-4694	en_US
dc.contributor.author	Doble, PA https://orcid.org/0000-0002-8472-1301	en_US
dc.date.available	2017-03-14	en_US
dc.date.issued	2017-05-12	en_US
dc.identifier.citation	Journal of Chromatography A, 2017, 1497 pp. 64 - 69	en_US
dc.identifier.issn	0021-9673	en_US
dc.identifier.uri	http://hdl.handle.net/10453/125150
dc.description.abstract	© 2017 Elsevier B.V. The Agilent Chip Cube Interface is a microfluidic chip-based technology originally designed for nanospray molecular mass spectrometry in which the sample enrichment, nano-column, tubing, connectors and spray tip were integrated into a single biocompatible chip. Here we describe the hyphenation of the Chip Cube Interface to ICP-MS via modification of the standard HPLC chip design and a new total consumption nebuliser suitable for flow rates as low as 300 nL min−1. The potential of the instrument to eliminate common nanoLC – ICP-MS shortcomings such as leaks, blockages and band-broadening was demonstrated via analysis of cyanocobalamin in equine plasma. The method was linear over three orders of magnitude with an r2 of 0.9999, the peak area repeatability was 1.9% (n = 7), and the detection limit was 14 ng mL−1. This novel configuration of the Chip Cube Interface coupled to ICP-MS is a suitable platform for the analysis of biomolecules associated with trace metals and speciation applications.	en_US
dc.relation.ispartof	Journal of Chromatography A	en_US
dc.relation.isbasedon	10.1016/j.chroma.2017.03.025	en_US
dc.subject.classification	Analytical Chemistry	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Horses	en_US
dc.subject.mesh	Vitamin B 12	en_US
dc.subject.mesh	Chromatography, High Pressure Liquid	en_US
dc.subject.mesh	Microfluidics	en_US
dc.subject.mesh	Mass Spectrometry	en_US
dc.subject.mesh	Limit of Detection	en_US
dc.title	Microfluidic high performance liquid chromatography-chip hyphenation to inductively coupled plasma–mass spectrometry	en_US
dc.type	Journal Article
utslib.citation.volume	1497	en_US
utslib.for	0303 Macromolecular and Materials Chemistry	en_US
utslib.for	0912 Materials Engineering	en_US
utslib.for	1007 Nanotechnology	en_US
utslib.for	03 Chemical Sciences	en_US
utslib.for	09 Engineering	en_US
utslib.for	10 Technology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - CFS - Centre for Forensic Science
pubs.organisational-group	/University of Technology Sydney/Students
utslib.copyright.status	closed_access
pubs.publication-status	Published	en_US
pubs.volume	1497	en_US

Abstract:

© 2017 Elsevier B.V. The Agilent Chip Cube Interface is a microfluidic chip-based technology originally designed for nanospray molecular mass spectrometry in which the sample enrichment, nano-column, tubing, connectors and spray tip were integrated into a single biocompatible chip. Here we describe the hyphenation of the Chip Cube Interface to ICP-MS via modification of the standard HPLC chip design and a new total consumption nebuliser suitable for flow rates as low as 300 nL min−1. The potential of the instrument to eliminate common nanoLC – ICP-MS shortcomings such as leaks, blockages and band-broadening was demonstrated via analysis of cyanocobalamin in equine plasma. The method was linear over three orders of magnitude with an r2 of 0.9999, the peak area repeatability was 1.9% (n = 7), and the detection limit was 14 ng mL−1. This novel configuration of the Chip Cube Interface coupled to ICP-MS is a suitable platform for the analysis of biomolecules associated with trace metals and speciation applications.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/125150