The First Isolation and Characterisation of the Protist Labyrinthula sp. in Southeastern Australia

Sullivan, BK; Robinson, KL; Trevathan-Tackett, SM; Lilje, ES; Gleason, FH; Lilje, O

The First Isolation and Characterisation of the Protist Labyrinthula sp. in Southeastern Australia

Sullivan, BK Robinson, KL Trevathan-Tackett, SM

Lilje, ES Gleason, FH Lilje, O

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Publication Type:: Journal Article
Citation:: Journal of Eukaryotic Microbiology, 2017, 64 (4), pp. 504 - 513
Issue Date:: 2017-07-01

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Field	Value	Language
dc.contributor.author	Sullivan, BK	en_US
dc.contributor.author	Robinson, KL	en_US
dc.contributor.author	Trevathan-Tackett, SM https://orcid.org/0000-0002-4977-0757	en_US
dc.contributor.author	Lilje, ES	en_US
dc.contributor.author	Gleason, FH	en_US
dc.contributor.author	Lilje, O	en_US
dc.date.available	2016-11-22	en_US
dc.date.issued	2017-07-01	en_US
dc.identifier.citation	Journal of Eukaryotic Microbiology, 2017, 64 (4), pp. 504 - 513	en_US
dc.identifier.issn	1066-5234	en_US
dc.identifier.uri	http://hdl.handle.net/10453/125153
dc.description.abstract	© 2016 The Author(s) Journal of Eukaryotic Microbiology © 2016 International Society of Protistologists As a result of anthropogenic influences and global climate change, emerging infectious marine diseases are thought to be increasingly more common and more severe than in the past. The aim of our investigation was to confirm the presence of Labyrinthula, the aetiological agent of the seagrass wasting disease, in Southeastern Australia and provide the first isolation and characterisation of this protist, in Australia. Colonies and individual cells were positively identified as Labyrinthula using published descriptions, diagrams, and photographs. Their identity was then confirmed using DNA barcoding of a region of the 18S rRNA gene. Species level identification of isolates was not possible as the taxonomy of the Labyrinthula is still poorly resolved. Still, a diversity of Labyrinthula was isolated from small sections of the southeast coast of Australia. The isolates were grouped into three haplotypes that are biogeographically restricted. These haplotypes are closely related to previously identified saprotrophic clades. The study highlights the need for further investigation into the global distribution of Labyrinthula, including phylogenetic pathogenicity and analysis of host-parasite interactions in response to stressors. Given the results of our analyses, it is prudent to continue research into disease and epidemic agents to better prepare researchers for potential future outbreaks.	en_US
dc.relation.ispartof	Journal of Eukaryotic Microbiology	en_US
dc.relation.isbasedon	10.1111/jeu.12387	en_US
dc.subject.classification	Mycology & Parasitology	en_US
dc.subject.mesh	Australia	en_US
dc.subject.mesh	Climate Change	en_US
dc.subject.mesh	DNA Barcoding, Taxonomic	en_US
dc.subject.mesh	DNA, Algal	en_US
dc.subject.mesh	DNA, Ribosomal	en_US
dc.subject.mesh	Haplotypes	en_US
dc.subject.mesh	Host-Parasite Interactions	en_US
dc.subject.mesh	Magnoliopsida	en_US
dc.subject.mesh	Phylogeny	en_US
dc.subject.mesh	RNA, Ribosomal, 18S	en_US
dc.subject.mesh	Sequence Analysis, DNA	en_US
dc.subject.mesh	Stramenopiles	en_US
dc.title	The First Isolation and Characterisation of the Protist Labyrinthula sp. in Southeastern Australia	en_US
dc.type	Journal Article
utslib.citation.volume	4	en_US
utslib.citation.volume	64	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	0608 Zoology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
utslib.copyright.status	closed_access
pubs.issue	4	en_US
pubs.publication-status	Published	en_US
pubs.volume	64	en_US

Abstract:

© 2016 The Author(s) Journal of Eukaryotic Microbiology © 2016 International Society of Protistologists As a result of anthropogenic influences and global climate change, emerging infectious marine diseases are thought to be increasingly more common and more severe than in the past. The aim of our investigation was to confirm the presence of Labyrinthula, the aetiological agent of the seagrass wasting disease, in Southeastern Australia and provide the first isolation and characterisation of this protist, in Australia. Colonies and individual cells were positively identified as Labyrinthula using published descriptions, diagrams, and photographs. Their identity was then confirmed using DNA barcoding of a region of the 18S rRNA gene. Species level identification of isolates was not possible as the taxonomy of the Labyrinthula is still poorly resolved. Still, a diversity of Labyrinthula was isolated from small sections of the southeast coast of Australia. The isolates were grouped into three haplotypes that are biogeographically restricted. These haplotypes are closely related to previously identified saprotrophic clades. The study highlights the need for further investigation into the global distribution of Labyrinthula, including phylogenetic pathogenicity and analysis of host-parasite interactions in response to stressors. Given the results of our analyses, it is prudent to continue research into disease and epidemic agents to better prepare researchers for potential future outbreaks.

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http://hdl.handle.net/10453/125153