Neural progenitor cells but not astrocytes respond distally to thoracic spinal cord injury in rat models

Nguyen, T; Mao, Y; Sutherland, T; Gorrie, CA

Neural progenitor cells but not astrocytes respond distally to thoracic spinal cord injury in rat models

Nguyen, T

Mao, Y

Sutherland, T

Gorrie, CA

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Publication Type:: Journal Article
Citation:: Neural Regeneration Research, 2017, 12 (11), pp. 1885 - 1894
Issue Date:: 2017-11-01

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Field	Value	Language
dc.contributor.author	Nguyen, T https://orcid.org/0000-0001-6371-4105	en_US
dc.contributor.author	Mao, Y https://orcid.org/0000-0002-3573-6486	en_US
dc.contributor.author	Sutherland, T https://orcid.org/0000-0002-2919-1504	en_US
dc.contributor.author	Gorrie, CA https://orcid.org/0000-0001-5934-2492	en_US
dc.date.issued	2017-11-01	en_US
dc.identifier.citation	Neural Regeneration Research, 2017, 12 (11), pp. 1885 - 1894	en_US
dc.identifier.issn	1673-5374	en_US
dc.identifier.uri	http://hdl.handle.net/10453/125169
dc.description.abstract	© 2017 Medknow Publications. All rights reserved. Traumatic spinal cord injury (SCI) is a detrimental condition that causes loss of sensory and motor function in an individual. Many complex secondary injury cascades occur after SCI and they offer great potential for therapeutic targeting. In this study, we investigated the response of endogenous neural progenitor cells, astrocytes, and microglia to a localized thoracic SCI throughout the neuroaxis. Twenty-five adult female Sprague-Dawley rats underwent mild-contusion thoracic SCI (n = 9), sham surgery (n = 8), or no surgery (n = 8). Spinal cord and brain tissues were fixed and cut at six regions of the neuroaxis. Immunohistochemistry showed increased reactivity of neural progenitor cell marker nestin in the central canal at all levels of the spinal cord. Increased reactivity of astrocyte-specific marker glial fibrillary acidic protein was found only at the lesion epicenter. The number of activated microglia was significantly increased at the lesion site, and activated microglia extended to the lumbar enlargement. Phagocytic microglia and macrophages were significantly increased only at the lesion site. There were no changes in nestin, glial fibrillary acidic protein, microglia and macrophage response in the third ventricle of rats subjected to mild-contusion thoracic SCI compared to the sham surgery or no surgery. These findings indicate that neural progenitor cells, astrocytes and microglia respond differently to a localized SCI, presumably due to differences in inflammatory signaling. These different cellular responses may have implications in the way that neural progenitor cells can be manipulated for neuroregeneration after SCI. This needs to be further investigated.	en_US
dc.relation.ispartof	Neural Regeneration Research	en_US
dc.relation.isbasedon	10.4103/1673-5374.219051	en_US
dc.title	Neural progenitor cells but not astrocytes respond distally to thoracic spinal cord injury in rat models	en_US
dc.type	Journal Article
utslib.citation.volume	11	en_US
utslib.citation.volume	12	en_US
utslib.for	1109 Neurosciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
utslib.copyright.status	open_access
pubs.issue	11	en_US
pubs.publication-status	Published	en_US
pubs.volume	12	en_US

Abstract:

© 2017 Medknow Publications. All rights reserved. Traumatic spinal cord injury (SCI) is a detrimental condition that causes loss of sensory and motor function in an individual. Many complex secondary injury cascades occur after SCI and they offer great potential for therapeutic targeting. In this study, we investigated the response of endogenous neural progenitor cells, astrocytes, and microglia to a localized thoracic SCI throughout the neuroaxis. Twenty-five adult female Sprague-Dawley rats underwent mild-contusion thoracic SCI (n = 9), sham surgery (n = 8), or no surgery (n = 8). Spinal cord and brain tissues were fixed and cut at six regions of the neuroaxis. Immunohistochemistry showed increased reactivity of neural progenitor cell marker nestin in the central canal at all levels of the spinal cord. Increased reactivity of astrocyte-specific marker glial fibrillary acidic protein was found only at the lesion epicenter. The number of activated microglia was significantly increased at the lesion site, and activated microglia extended to the lumbar enlargement. Phagocytic microglia and macrophages were significantly increased only at the lesion site. There were no changes in nestin, glial fibrillary acidic protein, microglia and macrophage response in the third ventricle of rats subjected to mild-contusion thoracic SCI compared to the sham surgery or no surgery. These findings indicate that neural progenitor cells, astrocytes and microglia respond differently to a localized SCI, presumably due to differences in inflammatory signaling. These different cellular responses may have implications in the way that neural progenitor cells can be manipulated for neuroregeneration after SCI. This needs to be further investigated.

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http://hdl.handle.net/10453/125169