para-Sulfonatocalix[4]arene and polyamidoamine dendrimer nanocomplexes as delivery vehicles for a novel platinum anticancer agent

Pang, CT; Ammit, AJ; Ong, YQE; Wheate, NJ

para-Sulfonatocalix[4]arene and polyamidoamine dendrimer nanocomplexes as delivery vehicles for a novel platinum anticancer agent

Pang, CT Ammit, AJ

Ong, YQE Wheate, NJ

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Publication Type:: Journal Article
Citation:: Journal of Inorganic Biochemistry, 2017, 176 pp. 1 - 7
Issue Date:: 2017-11-01

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Field	Value	Language
dc.contributor.author	Pang, CT	en_US
dc.contributor.author	Ammit, AJ https://orcid.org/0000-0003-0555-2544	en_US
dc.contributor.author	Ong, YQE	en_US
dc.contributor.author	Wheate, NJ	en_US
dc.date.available	2017-08-04	en_US
dc.date.issued	2017-11-01	en_US
dc.identifier.citation	Journal of Inorganic Biochemistry, 2017, 176 pp. 1 - 7	en_US
dc.identifier.issn	0162-0134	en_US
dc.identifier.uri	http://hdl.handle.net/10453/125183
dc.description.abstract	© 2017 Novel para-sulfonatocalix[4]arene (sCX[4]) and polyamidoamine (PAMAM) dendrimer nanocomplexes were evaluated as delivery vehicles for the platinum anticancer agent [(1,10-phenanthroline)(1S,2S-diaminocyclohexane)platinum(II)] chloride (PHENSS). Different ratios of sCX[4] to PHENSS were tested for their compatibility, with a ratio of 6:1 sCX[4]:PHENSS having the best solubility. The loading of sCX[4], and sCX[4]-bound PHENSS, onto three different generations of PAMAM dendrimers (G3.0–5.0) was examined using UV–visible spectrophotometry. The quantity of sCX[4] bound was found to increase exponentially with dendrimer size: G3, 15 sCX[4] molecules per dendrimer; G4, 37; and G5, 78. Similarly, the loading of sCX[4]-bound PHENSS also increased with increasing dendrimer size: G3, 7 PHENSS molecules per dendrimer; G4, 14; and G5, 28.5. The loading of sCX[4]-bound PHENSS molecules is significantly lower when compared with that of sCX[4], which indicates that less than half of the binding sites were occupied (45, 44, and 44%, respectively). By 1H NMR and UV–vis analysis, the nanocomplex was found to be stable in NaCl solutions at concentrations up to 150 mM. While PHENSS is more active in vitro than cisplatin against the human breast cancer cell line, MCF-7, delivery of PHENSS using the sCX[4]-dendrimer nanocomplexes, regardless of dendrimer generation, had little effect on PHENSS cytotoxicity. The results of this study may have application in the delivery of a variety of small molecule metal-based drugs for which chemical conjugation to a nanoparticle is undesired or not feasible.	en_US
dc.relation.ispartof	Journal of Inorganic Biochemistry	en_US
dc.relation.isbasedon	10.1016/j.jinorgbio.2017.08.002	en_US
dc.subject.classification	Inorganic & Nuclear Chemistry	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Breast Neoplasms	en_US
dc.subject.mesh	Polyamines	en_US
dc.subject.mesh	Organoplatinum Compounds	en_US
dc.subject.mesh	Antineoplastic Agents	en_US
dc.subject.mesh	Drug Screening Assays, Antitumor	en_US
dc.subject.mesh	Female	en_US
dc.subject.mesh	Dendrimers	en_US
dc.subject.mesh	MCF-7 Cells	en_US
dc.title	para-Sulfonatocalix[4]arene and polyamidoamine dendrimer nanocomplexes as delivery vehicles for a novel platinum anticancer agent	en_US
dc.type	Journal Article
utslib.citation.volume	176	en_US
utslib.for	0302 Inorganic Chemistry	en_US
utslib.for	0307 Theoretical and Computational Chemistry	en_US
utslib.for	0399 Other Chemical Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
utslib.copyright.status	closed_access
pubs.publication-status	Published	en_US
pubs.volume	176	en_US

Abstract:

© 2017 Novel para-sulfonatocalix[4]arene (sCX[4]) and polyamidoamine (PAMAM) dendrimer nanocomplexes were evaluated as delivery vehicles for the platinum anticancer agent [(1,10-phenanthroline)(1S,2S-diaminocyclohexane)platinum(II)] chloride (PHENSS). Different ratios of sCX[4] to PHENSS were tested for their compatibility, with a ratio of 6:1 sCX[4]:PHENSS having the best solubility. The loading of sCX[4], and sCX[4]-bound PHENSS, onto three different generations of PAMAM dendrimers (G3.0–5.0) was examined using UV–visible spectrophotometry. The quantity of sCX[4] bound was found to increase exponentially with dendrimer size: G3, 15 sCX[4] molecules per dendrimer; G4, 37; and G5, 78. Similarly, the loading of sCX[4]-bound PHENSS also increased with increasing dendrimer size: G3, 7 PHENSS molecules per dendrimer; G4, 14; and G5, 28.5. The loading of sCX[4]-bound PHENSS molecules is significantly lower when compared with that of sCX[4], which indicates that less than half of the binding sites were occupied (45, 44, and 44%, respectively). By 1H NMR and UV–vis analysis, the nanocomplex was found to be stable in NaCl solutions at concentrations up to 150 mM. While PHENSS is more active in vitro than cisplatin against the human breast cancer cell line, MCF-7, delivery of PHENSS using the sCX[4]-dendrimer nanocomplexes, regardless of dendrimer generation, had little effect on PHENSS cytotoxicity. The results of this study may have application in the delivery of a variety of small molecule metal-based drugs for which chemical conjugation to a nanoparticle is undesired or not feasible.

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http://hdl.handle.net/10453/125183