Resistance wheel exercise from mid-life has minimal effect on sciatic nerves from old mice in which sarcopenia was prevented

Krishnan, VS; White, Z; Terrill, JR; Hodgetts, SI; Fitzgerald, M; Shavlakadze, T; Harvey, AR; Grounds, MD

Resistance wheel exercise from mid-life has minimal effect on sciatic nerves from old mice in which sarcopenia was prevented

Krishnan, VS White, Z Terrill, JR Hodgetts, SI

Fitzgerald, M Shavlakadze, T Harvey, AR Grounds, MD

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Publication Type:: Journal Article
Citation:: Biogerontology, 2017, 18 (5), pp. 769 - 790
Issue Date:: 2017-10-01

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Field	Value	Language
dc.contributor.author	Krishnan, VS	en_US
dc.contributor.author	White, Z	en_US
dc.contributor.author	Terrill, JR	en_US
dc.contributor.author	Hodgetts, SI https://orcid.org/0000-0002-3318-0410	en_US
dc.contributor.author	Fitzgerald, M	en_US
dc.contributor.author	Shavlakadze, T	en_US
dc.contributor.author	Harvey, AR	en_US
dc.contributor.author	Grounds, MD	en_US
dc.date.available	2017-05-31	en_US
dc.date.issued	2017-10-01	en_US
dc.identifier.citation	Biogerontology, 2017, 18 (5), pp. 769 - 790	en_US
dc.identifier.issn	1389-5729	en_US
dc.identifier.uri	http://hdl.handle.net/10453/125253
dc.description.abstract	© 2017, Springer Science+Business Media B.V. The ability of resistance exercise, initiated from mid-life, to prevent age-related changes in old sciatic nerves, was investigated in male and female C57BL/6J mice. Aging is associated with cellular changes in old sciatic nerves and also loss of skeletal muscle mass and function (sarcopenia). Mature adult mice aged 15 months (M) were subjected to increasing voluntary resistance wheel exercise (RWE) over a period of 8 M until 23 M of age. This prevented sarcopenia in the old 23 M aged male and female mice. Nerves of control sedentary (SED) males at 3, 15 and 23 M of age, showed a decrease in the myelinated axon numbers at 15 and 23 M, a decreased g-ratio and a significantly increased proportion of myelinated nerves containing electron-dense aggregates at 23 M. Myelinated axon and nerve diameter, and axonal area, were increased at 15 M compared with 3 and 23 M. Exercise increased myelinated nerve profiles containing aggregates at 23 M. S100 protein, detected with immunoblotting was increased in sciatic nerves of 23 M old SED females, but not males, compared with 15 M, with no effect of exercise. Other neuronal proteins showed no significant alterations with age, gender or exercise. Overall the RWE had no cellular impact on the aging nerves, apart from an increased number of old nerves containing aggregates. Thus the relationship between cellular changes in aging nerves, and their sustained capacity for stimulation of old skeletal muscles to help maintain healthy muscle mass in response to exercise remains unclear.	en_US
dc.relation.ispartof	Biogerontology	en_US
dc.relation.isbasedon	10.1007/s10522-017-9714-8	en_US
dc.subject.classification	Gerontology	en_US
dc.subject.mesh	Sciatic Nerve	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Mice, Inbred C57BL	en_US
dc.subject.mesh	Mice	en_US
dc.subject.mesh	Physical Conditioning, Animal	en_US
dc.subject.mesh	Aging	en_US
dc.subject.mesh	Female	en_US
dc.subject.mesh	Male	en_US
dc.subject.mesh	Sarcopenia	en_US
dc.title	Resistance wheel exercise from mid-life has minimal effect on sciatic nerves from old mice in which sarcopenia was prevented	en_US
dc.type	Journal Article
utslib.citation.volume	5	en_US
utslib.citation.volume	18	en_US
utslib.for	1103 Clinical Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
utslib.copyright.status	closed_access
pubs.issue	5	en_US
pubs.publication-status	Published	en_US
pubs.volume	18	en_US

Abstract:

© 2017, Springer Science+Business Media B.V. The ability of resistance exercise, initiated from mid-life, to prevent age-related changes in old sciatic nerves, was investigated in male and female C57BL/6J mice. Aging is associated with cellular changes in old sciatic nerves and also loss of skeletal muscle mass and function (sarcopenia). Mature adult mice aged 15 months (M) were subjected to increasing voluntary resistance wheel exercise (RWE) over a period of 8 M until 23 M of age. This prevented sarcopenia in the old 23 M aged male and female mice. Nerves of control sedentary (SED) males at 3, 15 and 23 M of age, showed a decrease in the myelinated axon numbers at 15 and 23 M, a decreased g-ratio and a significantly increased proportion of myelinated nerves containing electron-dense aggregates at 23 M. Myelinated axon and nerve diameter, and axonal area, were increased at 15 M compared with 3 and 23 M. Exercise increased myelinated nerve profiles containing aggregates at 23 M. S100 protein, detected with immunoblotting was increased in sciatic nerves of 23 M old SED females, but not males, compared with 15 M, with no effect of exercise. Other neuronal proteins showed no significant alterations with age, gender or exercise. Overall the RWE had no cellular impact on the aging nerves, apart from an increased number of old nerves containing aggregates. Thus the relationship between cellular changes in aging nerves, and their sustained capacity for stimulation of old skeletal muscles to help maintain healthy muscle mass in response to exercise remains unclear.

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http://hdl.handle.net/10453/125253