Toxicology of gambierdiscus spp. (dinophyceae) from tropical and temperate Australian waters

Larsson, ME; Laczka, OF; Tim Harwood, D; Lewis, RJ; Himaya, SWA; Murray, SA; Doblin, MA

Toxicology of gambierdiscus spp. (dinophyceae) from tropical and temperate Australian waters

Larsson, ME

Laczka, OF

Tim Harwood, D Lewis, RJ Himaya, SWA Murray, SA

Doblin, MA

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Publication Type:: Journal Article
Citation:: Marine Drugs, 2018, 16 (1)
Issue Date:: 2018-01-01

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Field	Value	Language
dc.contributor.author	Larsson, ME https://orcid.org/0000-0003-4310-4582	en_US
dc.contributor.author	Laczka, OF https://orcid.org/0000-0003-0966-9897	en_US
dc.contributor.author	Tim Harwood, D	en_US
dc.contributor.author	Lewis, RJ	en_US
dc.contributor.author	Himaya, SWA	en_US
dc.contributor.author	Murray, SA https://orcid.org/0000-0001-7096-1307	en_US
dc.contributor.author	Doblin, MA https://orcid.org/0000-0001-8750-3433	en_US
dc.date.available	2017-12-15	en_US
dc.date.issued	2018-01-01	en_US
dc.identifier.citation	Marine Drugs, 2018, 16 (1)	en_US
dc.identifier.uri	http://hdl.handle.net/10453/125406
dc.description.abstract	© 2018 by the authors. Ciguatera Fish Poisoning (CFP) is a human illness caused by the consumption of marine fish contaminated with ciguatoxins (CTX) and possibly maitotoxins (MTX), produced by species from the benthic dinoflagellate genus Gambierdiscus. Here, we describe the identity and toxicology of Gambierdiscus spp. isolated from the tropical and temperate waters of eastern Australia. Based on newly cultured strains, we found that four Gambierdiscus species were present at the tropical location, including G. carpenteri, G. lapillus and two others which were not genetically identical to other currently described species within the genus, and may represent new species. Only G. carpenteri was identified from the temperate location. Using LC-MS/MS analysis we did not find any characterized microalgal CTXs (P-CTX-3B, P-CTX-3C, P-CTX-4A and P-CTX-4B) or MTX-1; however, putative maitotoxin-3 (MTX-3) was detected in all species except for the temperate population of G. carpenteri. Using the Ca2+ influx SH-SY5Y cell Fluorescent Imaging Plate Reader (FLIPR) bioassay we found CTX-like activity in extracts of the unidentified Gambierdiscus strains and trace level activity in strains of G. lapillus. While no detectable CTX-like activity was observed in tropical or temperate strains of G. carpenteri, all species showed strong maitotoxin-like activity. This study, which represents the most comprehensive analyses of the toxicology of Gambierdiscus strains isolated from Australia to date, suggests that CFP in this region may be caused by currently undescribed ciguatoxins and maitotoxins.	en_US
dc.relation.ispartof	Marine Drugs	en_US
dc.relation.isbasedon	10.3390/md16010007	en_US
dc.subject.classification	Medicinal & Biomolecular Chemistry	en_US
dc.subject.mesh	Cell Line, Tumor	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Dinoflagellida	en_US
dc.subject.mesh	Ciguatera Poisoning	en_US
dc.subject.mesh	Ciguatoxins	en_US
dc.subject.mesh	Oxocins	en_US
dc.subject.mesh	Marine Toxins	en_US
dc.subject.mesh	Chromatography, Liquid	en_US
dc.subject.mesh	Tropical Climate	en_US
dc.subject.mesh	Australia	en_US
dc.subject.mesh	Tandem Mass Spectrometry	en_US
dc.title	Toxicology of gambierdiscus spp. (dinophyceae) from tropical and temperate Australian waters	en_US
dc.type	Journal Article
utslib.citation.volume	1	en_US
utslib.citation.volume	16	en_US
utslib.for	1115 Pharmacology and Pharmaceutical Sciences	en_US
utslib.for	0306 Physical Chemistry (incl. Structural)	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	open_access
pubs.issue	1	en_US
pubs.publication-status	Published	en_US
pubs.volume	16	en_US

Abstract:

© 2018 by the authors. Ciguatera Fish Poisoning (CFP) is a human illness caused by the consumption of marine fish contaminated with ciguatoxins (CTX) and possibly maitotoxins (MTX), produced by species from the benthic dinoflagellate genus Gambierdiscus. Here, we describe the identity and toxicology of Gambierdiscus spp. isolated from the tropical and temperate waters of eastern Australia. Based on newly cultured strains, we found that four Gambierdiscus species were present at the tropical location, including G. carpenteri, G. lapillus and two others which were not genetically identical to other currently described species within the genus, and may represent new species. Only G. carpenteri was identified from the temperate location. Using LC-MS/MS analysis we did not find any characterized microalgal CTXs (P-CTX-3B, P-CTX-3C, P-CTX-4A and P-CTX-4B) or MTX-1; however, putative maitotoxin-3 (MTX-3) was detected in all species except for the temperate population of G. carpenteri. Using the Ca2+ influx SH-SY5Y cell Fluorescent Imaging Plate Reader (FLIPR) bioassay we found CTX-like activity in extracts of the unidentified Gambierdiscus strains and trace level activity in strains of G. lapillus. While no detectable CTX-like activity was observed in tropical or temperate strains of G. carpenteri, all species showed strong maitotoxin-like activity. This study, which represents the most comprehensive analyses of the toxicology of Gambierdiscus strains isolated from Australia to date, suggests that CFP in this region may be caused by currently undescribed ciguatoxins and maitotoxins.

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http://hdl.handle.net/10453/125406