Numerical simulation of the behavior of toroidal and spheroidal multicellular aggregates in microfluidic devices with microwell and U-shaped barrier

Barisam, M; Saidi, MS; Kashaninejad, N; Vadivelu, R; Nguyen, NT

Numerical simulation of the behavior of toroidal and spheroidal multicellular aggregates in microfluidic devices with microwell and U-shaped barrier

Barisam, M Saidi, MS Kashaninejad, N

Vadivelu, R Nguyen, NT

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Publication Type:: Journal Article
Citation:: Micromachines, 2017, 8 (12)
Issue Date:: 2017-12-11

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Field	Value	Language
dc.contributor.author	Barisam, M	en_US
dc.contributor.author	Saidi, MS	en_US
dc.contributor.author	Kashaninejad, N https://orcid.org/0000-0002-4385-7771	en_US
dc.contributor.author	Vadivelu, R	en_US
dc.contributor.author	Nguyen, NT	en_US
dc.date.available	2017-12-05	en_US
dc.date.issued	2017-12-11	en_US
dc.identifier.citation	Micromachines, 2017, 8 (12)	en_US
dc.identifier.uri	http://hdl.handle.net/10453/125482
dc.description.abstract	© 2017 by the authors. A microfluidic system provides an excellent platform for cellular studies. Most importantly, a three-dimensional (3D) cell culture model reconstructs more accurately the in vivo microenvironment of tissue. Accordingly, microfluidic 3D cell culture devices could be ideal candidates for in vitro cell culture platforms. In this paper, two types of 3D cellular aggregates, i.e., toroid and spheroid, are numerically studied. The studies are carried out for microfluidic systems containing U-shaped barrier as well as microwell structure. For the first time, we obtain oxygen and glucose concentration distributions inside a toroid aggregate as well as the shear stress on its surface and compare its performance with a spheroid aggregate of the same volume. In particular, we obtain the oxygen concentration distributions in three areas, namely, oxygen-permeable layer, multicellular aggregates and culture medium. Further, glucose concentration distributions in two regions of multicellular aggregates and culture medium are investigated. The results show that the levels of oxygen and glucose in the system containing U-shaped barriers are far more than those in the system containing microwells. Therefore, to achieve high levels of oxygen and nutrients, a system with U-shaped barriers is more suited than the conventional traps, but the choice between toroid and spheroid depends on their volume and orientation. The results indicate that higher oxygen and glucose concentrations can be achieved in spheroid with a small volume as well as in horizontal toroid with a large volume. The vertical toroid has the highest levels of oxygen and glucose concentration while the surface shear stress on its surface is also maximum. These findings can be used as guidelines for designing an optimum 3D microfluidic bioreactor based on the desired levels of oxygen, glucose and shear stress distributions.	en_US
dc.relation.ispartof	Micromachines	en_US
dc.relation.isbasedon	10.3390/mi8120358	en_US
dc.title	Numerical simulation of the behavior of toroidal and spheroidal multicellular aggregates in microfluidic devices with microwell and U-shaped barrier	en_US
dc.type	Journal Article
utslib.citation.volume	12	en_US
utslib.citation.volume	8	en_US
utslib.for	1007 Nanotechnology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
utslib.copyright.status	open_access
pubs.issue	12	en_US
pubs.publication-status	Published	en_US
pubs.volume	8	en_US

Abstract:

© 2017 by the authors. A microfluidic system provides an excellent platform for cellular studies. Most importantly, a three-dimensional (3D) cell culture model reconstructs more accurately the in vivo microenvironment of tissue. Accordingly, microfluidic 3D cell culture devices could be ideal candidates for in vitro cell culture platforms. In this paper, two types of 3D cellular aggregates, i.e., toroid and spheroid, are numerically studied. The studies are carried out for microfluidic systems containing U-shaped barrier as well as microwell structure. For the first time, we obtain oxygen and glucose concentration distributions inside a toroid aggregate as well as the shear stress on its surface and compare its performance with a spheroid aggregate of the same volume. In particular, we obtain the oxygen concentration distributions in three areas, namely, oxygen-permeable layer, multicellular aggregates and culture medium. Further, glucose concentration distributions in two regions of multicellular aggregates and culture medium are investigated. The results show that the levels of oxygen and glucose in the system containing U-shaped barriers are far more than those in the system containing microwells. Therefore, to achieve high levels of oxygen and nutrients, a system with U-shaped barriers is more suited than the conventional traps, but the choice between toroid and spheroid depends on their volume and orientation. The results indicate that higher oxygen and glucose concentrations can be achieved in spheroid with a small volume as well as in horizontal toroid with a large volume. The vertical toroid has the highest levels of oxygen and glucose concentration while the surface shear stress on its surface is also maximum. These findings can be used as guidelines for designing an optimum 3D microfluidic bioreactor based on the desired levels of oxygen, glucose and shear stress distributions.

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http://hdl.handle.net/10453/125482