Degradation of Adsorbed Azo Dye by Solid-State Fermentation: Improvement of Culture Conditions, a Kinetic Study, and Rotating Drum Bioreactor Performance

Jaramillo, AC; Cobas, M; Hormaza, A; Sanromán, MÁ

Degradation of Adsorbed Azo Dye by Solid-State Fermentation: Improvement of Culture Conditions, a Kinetic Study, and Rotating Drum Bioreactor Performance

Jaramillo, AC Cobas, M Hormaza, A Sanromán, MÁ

Permalink

Publication Type:: Journal Article
Citation:: Water, Air, and Soil Pollution, 2017, 228 (6)
Issue Date:: 2017-01-01

Closed Access

	Filename	Description	Size
	Jaramillo2017_Article_DegradationOfAdsorbedAzoDyeByS.pdf	Published Version	1.28 MB	Adobe PDF	View/Open

Copyright Clearance Process

Recently Added
In Progress
Closed Access

This item is closed access and not available.

Full metadata record

Field	Value	Language
dc.contributor.author	Jaramillo, AC	en_US
dc.contributor.author	Cobas, M	en_US
dc.contributor.author	Hormaza, A	en_US
dc.contributor.author	Sanromán, MÁ	en_US
dc.date.issued	2017-01-01	en_US
dc.identifier.citation	Water, Air, and Soil Pollution, 2017, 228 (6)	en_US
dc.identifier.issn	0049-6979	en_US
dc.identifier.uri	http://hdl.handle.net/10453/125691
dc.description.abstract	© 2017, Springer International Publishing Switzerland. The presence of synthetic dyes in effluents leads to an environmental imbalance characterized by a decrease in photosynthetic activity and, therefore, a reduction of available oxygen, which affects all living aquatic species. To reduce this problem, a combination adsorption and biodegradation treatment strategy is proposed. In this work, Red 40 dye was adsorbed onto a low-cost waste product, followed by degradation by Trametes versicolor under solid state fermentation conditions. The principal aim of this research was to establish the best fermentation conditions using a kinetic evaluation of both degradation and laccase enzyme activity. The process was scaled-up using a rotating drum bioreactor. The best process conditions were a carbon:nitrogen ratio of 30:1, a moisture percentage of 75%, and an inductor concentration of 0.5 mM; the maximum dye degradation was 96.04%. Under these optimized conditions, the highest enzymatic activity was 8.49 U/gdm after 14 days of culture at the flask scale. Using a rotating drum bioreactor, 630 mg of azo dye was degraded after 30 days of culture. Red 40 dye degradation was confirmed using infrared spectroscopy Fourier transform infrared spectrometer and HPLC-MS techniques. The results show that the degradation percentage has a direct relation with laccase activity, and the obtained efficiency in the rotating drum bioreactor confirms the potential of this methodology for implementation at the industrial level.	en_US
dc.relation.ispartof	Water, Air, and Soil Pollution	en_US
dc.relation.isbasedon	10.1007/s11270-017-3389-2	en_US
dc.subject.classification	Environmental Sciences	en_US
dc.title	Degradation of Adsorbed Azo Dye by Solid-State Fermentation: Improvement of Culture Conditions, a Kinetic Study, and Rotating Drum Bioreactor Performance	en_US
dc.type	Journal Article
utslib.citation.volume	6	en_US
utslib.citation.volume	228	en_US
utslib.for	0503 Soil Sciences	en_US
utslib.for	MD Multidisciplinary	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
utslib.copyright.status	closed_access
pubs.issue	6	en_US
pubs.publication-status	Published	en_US
pubs.volume	228	en_US

Abstract:

© 2017, Springer International Publishing Switzerland. The presence of synthetic dyes in effluents leads to an environmental imbalance characterized by a decrease in photosynthetic activity and, therefore, a reduction of available oxygen, which affects all living aquatic species. To reduce this problem, a combination adsorption and biodegradation treatment strategy is proposed. In this work, Red 40 dye was adsorbed onto a low-cost waste product, followed by degradation by Trametes versicolor under solid state fermentation conditions. The principal aim of this research was to establish the best fermentation conditions using a kinetic evaluation of both degradation and laccase enzyme activity. The process was scaled-up using a rotating drum bioreactor. The best process conditions were a carbon:nitrogen ratio of 30:1, a moisture percentage of 75%, and an inductor concentration of 0.5 mM; the maximum dye degradation was 96.04%. Under these optimized conditions, the highest enzymatic activity was 8.49 U/gdm after 14 days of culture at the flask scale. Using a rotating drum bioreactor, 630 mg of azo dye was degraded after 30 days of culture. Red 40 dye degradation was confirmed using infrared spectroscopy Fourier transform infrared spectrometer and HPLC-MS techniques. The results show that the degradation percentage has a direct relation with laccase activity, and the obtained efficiency in the rotating drum bioreactor confirms the potential of this methodology for implementation at the industrial level.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/125691