Chronic rhinosinusitis: Potential role of microbial dysbiosis and recommendations for sampling sites

Copeland, E; Leonard, K; Carney, R; Kong, J; Forer, M; Naidoo, Y; Oliver, BGG; Seymour, JR; Woodcock, S; Burke, CM; Stow, NW

Chronic rhinosinusitis: Potential role of microbial dysbiosis and recommendations for sampling sites

Copeland, E Leonard, K Carney, R Kong, J Forer, M Naidoo, Y Oliver, BGG

Seymour, JR

Woodcock, S

Burke, CM

Stow, NW

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Publication Type:: Journal Article
Citation:: Frontiers in Cellular and Infection Microbiology, 2018, 8 (FEB)
Issue Date:: 2018-02-28

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Field	Value	Language
dc.contributor.author	Copeland, E	en_US
dc.contributor.author	Leonard, K	en_US
dc.contributor.author	Carney, R	en_US
dc.contributor.author	Kong, J	en_US
dc.contributor.author	Forer, M	en_US
dc.contributor.author	Naidoo, Y	en_US
dc.contributor.author	Oliver, BGG https://orcid.org/0000-0002-7122-9262	en_US
dc.contributor.author	Seymour, JR https://orcid.org/0000-0002-3745-6541	en_US
dc.contributor.author	Woodcock, S https://orcid.org/0000-0003-4903-3164	en_US
dc.contributor.author	Burke, CM https://orcid.org/0000-0002-8917-0019	en_US
dc.contributor.author	Stow, NW	en_US
dc.date.available	2018-02-12	en_US
dc.date.issued	2018-02-28	en_US
dc.identifier.citation	Frontiers in Cellular and Infection Microbiology, 2018, 8 (FEB)	en_US
dc.identifier.uri	http://hdl.handle.net/10453/126134
dc.description.abstract	© 2018 Copeland, Leonard, Carney, Kong, Forer, Naidoo, Oliver, Seymour, Woodcock, Burke and Stow. Chronic rhinosinusitis (CRS) is an inflammatory condition that affects up to 12% of the human population in developed countries. Previous studies examining the potential role of the sinus bacterial microbiota within CRS infections have found inconsistent results, possibly because of inconsistencies in sampling strategies. The aim of this study was to determine whether the sinus microbiome is altered in CRS and additionally if the middle meatus is a suitable representative site for sampling the sinus microbiome. Swab samples were collected from 12 healthy controls and 21 CRS patients, including all eight sinuses for CRS patients and between one and five sinuses for control subjects. The left and right middle meatus and nostril swabs were also collected. Significant differences in the sinus microbiomes between CRS and control samples were revealed using high-throughput 16S rRNA gene sequencing. The genus Escherichia was over-represented in CRS sinuses, and associations between control patients and Corynebacterium and Dolosigranulum were also identified. Comparisons of the middle meatuses between groups did not reflect these differences, and the abundance of the genus Escherichia was significantly lower at this location. Additionally, intra-patient variation was lower between sinuses than between sinus and middle meatus, which together with the above results suggests that the middle meatus is not an effective representative sampling site.	en_US
dc.relation.ispartof	Frontiers in Cellular and Infection Microbiology	en_US
dc.relation.isbasedon	10.3389/fcimb.2018.00057	en_US
dc.subject.mesh	Nasal Cavity	en_US
dc.subject.mesh	Paranasal Sinuses	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Bacteria	en_US
dc.subject.mesh	Escherichia	en_US
dc.subject.mesh	Sinusitis	en_US
dc.subject.mesh	Rhinitis	en_US
dc.subject.mesh	Chronic Disease	en_US
dc.subject.mesh	DNA, Bacterial	en_US
dc.subject.mesh	RNA, Ribosomal, 16S	en_US
dc.subject.mesh	Sequence Analysis	en_US
dc.subject.mesh	Biodiversity	en_US
dc.subject.mesh	Carnobacteriaceae	en_US
dc.subject.mesh	Microbiota	en_US
dc.subject.mesh	Dysbiosis	en_US
dc.title	Chronic rhinosinusitis: Potential role of microbial dysbiosis and recommendations for sampling sites	en_US
dc.type	Journal Article
utslib.citation.volume	FEB	en_US
utslib.citation.volume	8	en_US
utslib.for	0604 Genetics	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	0601 Biochemistry and Cell Biology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
utslib.copyright.status	open_access
pubs.issue	FEB	en_US
pubs.publication-status	Published	en_US
pubs.volume	8	en_US

Abstract:

© 2018 Copeland, Leonard, Carney, Kong, Forer, Naidoo, Oliver, Seymour, Woodcock, Burke and Stow. Chronic rhinosinusitis (CRS) is an inflammatory condition that affects up to 12% of the human population in developed countries. Previous studies examining the potential role of the sinus bacterial microbiota within CRS infections have found inconsistent results, possibly because of inconsistencies in sampling strategies. The aim of this study was to determine whether the sinus microbiome is altered in CRS and additionally if the middle meatus is a suitable representative site for sampling the sinus microbiome. Swab samples were collected from 12 healthy controls and 21 CRS patients, including all eight sinuses for CRS patients and between one and five sinuses for control subjects. The left and right middle meatus and nostril swabs were also collected. Significant differences in the sinus microbiomes between CRS and control samples were revealed using high-throughput 16S rRNA gene sequencing. The genus Escherichia was over-represented in CRS sinuses, and associations between control patients and Corynebacterium and Dolosigranulum were also identified. Comparisons of the middle meatuses between groups did not reflect these differences, and the abundance of the genus Escherichia was significantly lower at this location. Additionally, intra-patient variation was lower between sinuses than between sinus and middle meatus, which together with the above results suggests that the middle meatus is not an effective representative sampling site.

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http://hdl.handle.net/10453/126134