Structural properties of liposomes from digital holographic microscopy

Di Maio, IL; Carl, D; Langehanenberg, P; Valenzuela, SM; Battle, AR; Al Khazaaly, S; Killingsworth, M; Kemper, B; Von Bally, G; Martin, DK

Structural properties of liposomes from digital holographic microscopy

Di Maio, IL Carl, D Langehanenberg, P Valenzuela, SM

Battle, AR Al Khazaaly, S Killingsworth, M Kemper, B Von Bally, G Martin, DK

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Publication Type:: Conference Proceeding
Citation:: Progress in Biomedical Optics and Imaging - Proceedings of SPIE, 2006, 6036
Issue Date:: 2006-03-31

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Field	Value	Language
dc.contributor.author	Di Maio, IL	en_US
dc.contributor.author	Carl, D	en_US
dc.contributor.author	Langehanenberg, P	en_US
dc.contributor.author	Valenzuela, SM https://orcid.org/0000-0001-5934-6047	en_US
dc.contributor.author	Battle, AR	en_US
dc.contributor.author	Al Khazaaly, S	en_US
dc.contributor.author	Killingsworth, M	en_US
dc.contributor.author	Kemper, B	en_US
dc.contributor.author	Von Bally, G	en_US
dc.contributor.author	Martin, DK https://orcid.org/0000-0001-5913-2372	en_US
dc.date.issued	2006-03-31	en_US
dc.identifier.citation	Progress in Biomedical Optics and Imaging - Proceedings of SPIE, 2006, 6036	en_US
dc.identifier.issn	1605-7422	en_US
dc.identifier.uri	http://hdl.handle.net/10453/12696
dc.description.abstract	We have constructed liposomes from L-alpha-Phosphatidylcholine (PC) lipids, which are biomimetic lipids similar to those present in the membranes of mammalian cells. We propose an advance in the use of liposomes, such as for drug delivery, to incorporate into the liposomal membranes transport proteins that have been extracted from the lipid membranes of mammalian cells. In this paper, we describe the usage of a novel optical microscope to characterize the nanomechanical properties of these liposomes. We have applied the technique of digital holographic microscopy1, using an instrument recently developed at the University of Münster, Germany. This system enabled us to measure quantitatively the structural changes in liposomes. We have investigated the deformations of these biomimetic lipids comprising these liposomes by applying osmotic stresses, in order to gain insight into the membrane environment prior to incorporation of cloned membrane transport proteins. This control of the nanomechanical properties is important in the stresses transmitted to mechanosensitive ion channels that we have incorporated into the liposomal membranes. These liposomes provide transporting vesicles that respond to mechanical stresses, such as those that occur during implantation.	en_US
dc.relation.ispartof	Progress in Biomedical Optics and Imaging - Proceedings of SPIE	en_US
dc.relation.isbasedon	10.1117/12.638823	en_US
dc.title	Structural properties of liposomes from digital holographic microscopy	en_US
dc.type	Conference Proceeding
utslib.citation.volume	6036	en_US
utslib.for	0304 Medicinal and Biomolecular Chemistry	en_US
utslib.for	0306 Physical Chemistry (incl. Structural)	en_US
utslib.for	1004 Medical Biotechnology	en_US
dc.location.activity	Brisbane, AUSTRALIA	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Medical and Molecular Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
utslib.copyright.status	open_access
pubs.publication-status	Published	en_US
pubs.volume	6036	en_US

Abstract:

We have constructed liposomes from L-alpha-Phosphatidylcholine (PC) lipids, which are biomimetic lipids similar to those present in the membranes of mammalian cells. We propose an advance in the use of liposomes, such as for drug delivery, to incorporate into the liposomal membranes transport proteins that have been extracted from the lipid membranes of mammalian cells. In this paper, we describe the usage of a novel optical microscope to characterize the nanomechanical properties of these liposomes. We have applied the technique of digital holographic microscopy1, using an instrument recently developed at the University of Münster, Germany. This system enabled us to measure quantitatively the structural changes in liposomes. We have investigated the deformations of these biomimetic lipids comprising these liposomes by applying osmotic stresses, in order to gain insight into the membrane environment prior to incorporation of cloned membrane transport proteins. This control of the nanomechanical properties is important in the stresses transmitted to mechanosensitive ion channels that we have incorporated into the liposomal membranes. These liposomes provide transporting vesicles that respond to mechanical stresses, such as those that occur during implantation.

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http://hdl.handle.net/10453/12696