Multi-photon near-infrared emission saturation nanoscopy using upconversion nanoparticles

Chen, C; Wang, F; Wen, S; Su, QP; Wu, MCL; Liu, Y; Wang, B; Li, D; Shan, X; Kianinia, M; Aharonovich, I; Toth, M; Jackson, SP; Xi, P; Jin, D

Multi-photon near-infrared emission saturation nanoscopy using upconversion nanoparticles

Chen, C

Wang, F

Wen, S

Su, QP

Wu, MCL Liu, Y Wang, B

Li, D Shan, X

Kianinia, M Aharonovich, I

Toth, M

Jackson, SP Xi, P Jin, D

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Publication Type:: Journal Article
Citation:: Nature Communications, 2018, 9 (1)
Issue Date:: 2018-12-01

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Field	Value	Language
dc.contributor.author	Chen, C https://orcid.org/0000-0003-4620-7771	en_US
dc.contributor.author	Wang, F https://orcid.org/0000-0001-7403-3305	en_US
dc.contributor.author	Wen, S https://orcid.org/0000-0002-4670-4658	en_US
dc.contributor.author	Su, QP https://orcid.org/0000-0001-7364-3945	en_US
dc.contributor.author	Wu, MCL	en_US
dc.contributor.author	Liu, Y	en_US
dc.contributor.author	Wang, B https://orcid.org/0000-0002-8701-3896	en_US
dc.contributor.author	Li, D	en_US
dc.contributor.author	Shan, X https://orcid.org/0000-0001-7889-4006	en_US
dc.contributor.author	Kianinia, M	en_US
dc.contributor.author	Aharonovich, I https://orcid.org/0000-0003-4304-3935	en_US
dc.contributor.author	Toth, M https://orcid.org/0000-0003-1564-4899	en_US
dc.contributor.author	Jackson, SP	en_US
dc.contributor.author	Xi, P	en_US
dc.contributor.author	Jin, D https://orcid.org/0000-0003-1046-2666	en_US
dc.date.available	2018-07-26	en_US
dc.date.issued	2018-12-01	en_US
dc.identifier.citation	Nature Communications, 2018, 9 (1)	en_US
dc.identifier.uri	http://hdl.handle.net/10453/127638
dc.description.abstract	© 2018, The Author(s). Multiphoton fluorescence microscopy (MPM), using near infrared excitation light, provides increased penetration depth, decreased detection background, and reduced phototoxicity. Using stimulated emission depletion (STED) approach, MPM can bypass the diffraction limitation, but it requires both spatial alignment and temporal synchronization of high power (femtosecond) lasers, which is limited by the inefficiency of the probes. Here, we report that upconversion nanoparticles (UCNPs) can unlock a new mode of near-infrared emission saturation (NIRES) nanoscopy for deep tissue super-resolution imaging with excitation intensity several orders of magnitude lower than that required by conventional MPM dyes. Using a doughnut beam excitation from a 980 nm diode laser and detecting at 800 nm, we achieve a resolution of sub 50 nm, 1/20th of the excitation wavelength, in imaging of single UCNP through 93 μm thick liver tissue. This method offers a simple solution for deep tissue super resolution imaging and single molecule tracking.	en_US
dc.relation	http://purl.org/au-research/grants/arc/FT130100517
dc.relation	http://purl.org/au-research/grants/arc/DP180100077
dc.relation.ispartof	Nature Communications	en_US
dc.relation.isbasedon	10.1038/s41467-018-05842-w	en_US
dc.title	Multi-photon near-infrared emission saturation nanoscopy using upconversion nanoparticles	en_US
dc.type	Journal Article
utslib.citation.volume	1	en_US
utslib.citation.volume	9	en_US
utslib.for	1007 Nanotechnology	en_US
utslib.for	0205 Optical Physics	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
pubs.organisational-group	/University of Technology Sydney/Strength - MTEE - Research Centre Materials and Technology for Energy Efficiency
pubs.organisational-group	/University of Technology Sydney/Students
utslib.copyright.status	open_access
pubs.issue	1	en_US
pubs.publication-status	Published	en_US
pubs.volume	9	en_US

Abstract:

© 2018, The Author(s). Multiphoton fluorescence microscopy (MPM), using near infrared excitation light, provides increased penetration depth, decreased detection background, and reduced phototoxicity. Using stimulated emission depletion (STED) approach, MPM can bypass the diffraction limitation, but it requires both spatial alignment and temporal synchronization of high power (femtosecond) lasers, which is limited by the inefficiency of the probes. Here, we report that upconversion nanoparticles (UCNPs) can unlock a new mode of near-infrared emission saturation (NIRES) nanoscopy for deep tissue super-resolution imaging with excitation intensity several orders of magnitude lower than that required by conventional MPM dyes. Using a doughnut beam excitation from a 980 nm diode laser and detecting at 800 nm, we achieve a resolution of sub 50 nm, 1/20th of the excitation wavelength, in imaging of single UCNP through 93 μm thick liver tissue. This method offers a simple solution for deep tissue super resolution imaging and single molecule tracking.

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http://hdl.handle.net/10453/127638