Nitrogen fixation in a landrace of maize is supported by a mucilage-associated diazotrophic microbiota

Van Deynze, A; Zamora, P; Delaux, PM; Heitmann, C; Jayaraman, D; Rajasekar, S; Graham, D; Maeda, J; Gibson, D; Schwartz, KD; Berry, AM; Bhatnagar, S; Jospin, G; Darling, A; Jeannotte, R; Lopez, J; Weimer, BC; Eisen, JA; Shapiro, HY; Ané, JM; Bennett, AB

Nitrogen fixation in a landrace of maize is supported by a mucilage-associated diazotrophic microbiota

Van Deynze, A Zamora, P Delaux, PM Heitmann, C Jayaraman, D Rajasekar, S Graham, D Maeda, J Gibson, D Schwartz, KD Berry, AM Bhatnagar, S Jospin, G Darling, A

Jeannotte, R Lopez, J Weimer, BC Eisen, JA Shapiro, HY Ané, JM Bennett, AB

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Publication Type:: Journal Article
Citation:: PLoS Biology, 2018, 16 (8)
Issue Date:: 2018-08-07

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Field	Value	Language
dc.contributor.author	Van Deynze, A	en_US
dc.contributor.author	Zamora, P	en_US
dc.contributor.author	Delaux, PM	en_US
dc.contributor.author	Heitmann, C	en_US
dc.contributor.author	Jayaraman, D	en_US
dc.contributor.author	Rajasekar, S	en_US
dc.contributor.author	Graham, D	en_US
dc.contributor.author	Maeda, J	en_US
dc.contributor.author	Gibson, D	en_US
dc.contributor.author	Schwartz, KD	en_US
dc.contributor.author	Berry, AM	en_US
dc.contributor.author	Bhatnagar, S	en_US
dc.contributor.author	Jospin, G	en_US
dc.contributor.author	Darling, A https://orcid.org/0000-0003-2397-7925	en_US
dc.contributor.author	Jeannotte, R	en_US
dc.contributor.author	Lopez, J	en_US
dc.contributor.author	Weimer, BC	en_US
dc.contributor.author	Eisen, JA	en_US
dc.contributor.author	Shapiro, HY	en_US
dc.contributor.author	Ané, JM	en_US
dc.contributor.author	Bennett, AB	en_US
dc.date.available	2018-07-03	en_US
dc.date.issued	2018-08-07	en_US
dc.identifier.citation	PLoS Biology, 2018, 16 (8)	en_US
dc.identifier.issn	1544-9173	en_US
dc.identifier.uri	http://hdl.handle.net/10453/127768
dc.description.abstract	© 2018 Van Deynze et al. http://creativecommons.org/licenses/by/4.0/. Plants are associated with a complex microbiota that contributes to nutrient acquisition, plant growth, and plant defense. Nitrogen-fixing microbial associations are efficient and well characterized in legumes but are limited in cereals, including maize. We studied an indigenous landrace of maize grown in nitrogen-depleted soils in the Sierra Mixe region of Oaxaca, Mexico. This landrace is characterized by the extensive development of aerial roots that secrete a carbohydrate-rich mucilage. Analysis of the mucilage microbiota indicated that it was enriched in taxa for which many known species are diazotrophic, was enriched for homologs of genes encoding nitrogenase subunits, and harbored active nitrogenase activity as assessed by acetylene reduction and 15 N 2 incorporation assays. Field experiments in Sierra Mixe using 15 N natural abundance or 15 N-enrichment assessments over 5 years indicated that atmospheric nitrogen fixation contributed 29%–82% of the nitrogen nutrition of Sierra Mixe maize.	en_US
dc.relation.ispartof	PLoS Biology	en_US
dc.relation.isbasedon	10.1371/journal.pbio.2006352	en_US
dc.subject.classification	Developmental Biology	en_US
dc.subject.mesh	Zea mays	en_US
dc.subject.mesh	Plant Roots	en_US
dc.subject.mesh	Nitrogen	en_US
dc.subject.mesh	Polysaccharides	en_US
dc.subject.mesh	Soil	en_US
dc.subject.mesh	Soil Microbiology	en_US
dc.subject.mesh	Phylogeny	en_US
dc.subject.mesh	Nitrogen Fixation	en_US
dc.subject.mesh	Mexico	en_US
dc.subject.mesh	Plant Development	en_US
dc.subject.mesh	Plant Mucilage	en_US
dc.subject.mesh	Microbiota	en_US
dc.title	Nitrogen fixation in a landrace of maize is supported by a mucilage-associated diazotrophic microbiota	en_US
dc.type	Journal Article
utslib.citation.volume	8	en_US
utslib.citation.volume	16	en_US
utslib.for	0607 Plant Biology	en_US
utslib.for	0703 Crop and Pasture Production	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	07 Agricultural and Veterinary Sciences	en_US
utslib.for	11 Medical and Health Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	open_access
pubs.issue	8	en_US
pubs.publication-status	Published	en_US
pubs.volume	16	en_US

Abstract:

© 2018 Van Deynze et al. http://creativecommons.org/licenses/by/4.0/. Plants are associated with a complex microbiota that contributes to nutrient acquisition, plant growth, and plant defense. Nitrogen-fixing microbial associations are efficient and well characterized in legumes but are limited in cereals, including maize. We studied an indigenous landrace of maize grown in nitrogen-depleted soils in the Sierra Mixe region of Oaxaca, Mexico. This landrace is characterized by the extensive development of aerial roots that secrete a carbohydrate-rich mucilage. Analysis of the mucilage microbiota indicated that it was enriched in taxa for which many known species are diazotrophic, was enriched for homologs of genes encoding nitrogenase subunits, and harbored active nitrogenase activity as assessed by acetylene reduction and 15 N 2 incorporation assays. Field experiments in Sierra Mixe using 15 N natural abundance or 15 N-enrichment assessments over 5 years indicated that atmospheric nitrogen fixation contributed 29%–82% of the nitrogen nutrition of Sierra Mixe maize.

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http://hdl.handle.net/10453/127768