Characterization of naturally occurring, new and persistent subclinical foot-and-mouth disease virus infection in vaccinated Asian buffalo in Islamabad Capital Territory, Pakistan

Farooq, U; Ahmed, Z; Naeem, K; Bertram, M; Brito, B; Stenfeldt, C; Pauszek, SJ; LaRocco, M; Rodriguez, L; Arzt, J

Characterization of naturally occurring, new and persistent subclinical foot-and-mouth disease virus infection in vaccinated Asian buffalo in Islamabad Capital Territory, Pakistan

Farooq, U Ahmed, Z Naeem, K Bertram, M Brito, B

Stenfeldt, C Pauszek, SJ LaRocco, M Rodriguez, L Arzt, J

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Publication Type:: Journal Article
Citation:: Transboundary and Emerging Diseases, 2018, 65 (6), pp. 1836 - 1850
Issue Date:: 2018-12-01

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Field	Value	Language
dc.contributor.author	Farooq, U	en_US
dc.contributor.author	Ahmed, Z	en_US
dc.contributor.author	Naeem, K	en_US
dc.contributor.author	Bertram, M	en_US
dc.contributor.author	Brito, B https://orcid.org/0000-0001-6122-2596	en_US
dc.contributor.author	Stenfeldt, C	en_US
dc.contributor.author	Pauszek, SJ	en_US
dc.contributor.author	LaRocco, M	en_US
dc.contributor.author	Rodriguez, L	en_US
dc.contributor.author	Arzt, J	en_US
dc.date.available	2018-06-28	en_US
dc.date.issued	2018-12-01	en_US
dc.identifier.citation	Transboundary and Emerging Diseases, 2018, 65 (6), pp. 1836 - 1850	en_US
dc.identifier.issn	1865-1674	en_US
dc.identifier.uri	http://hdl.handle.net/10453/127863
dc.description.abstract	© 2018 Blackwell Verlag GmbH The convalescent subclinical carrier state of foot-and-mouth disease virus (FMDV) infection has been thoroughly investigated; contrastingly, the subclinical form of new infections of vaccinated and naïve hosts is recognized, but poorly understood. To investigate the natural dynamics of subclinical FMDV infections, a prospective, 12-month, longitudinal study was conducted in vaccinated Asian buffalo (Bubalus bubalis) under natural conditions in Pakistan, where FMDV is hyperendemic. Oropharyngeal fluid (OPF) samples were obtained quarterly from 300 buffalo on 30 farms which reported no clinical FMD during the 12-month study period. At the start of the study, 77.7% of buffalo had FMDV anti-NSP antibodies, and all farms had at least one seropositive buffalo. Based upon the presence of viral RNA and viral VP1 sequences obtained, distinct subcategories of subclinical infections were documented, including new, persistent, and serial infections with different FMDV strains. Viral RNA was detected in at least one OPF sample from 180 (60%) of the 300 buffalo. Over the course of the study, FMDV was detected in OPF of 80 buffalo that had been FMDV-free in previous OPF samples, indicating the occurrence of new subclinical infections. Eight buffalo were confirmed to be persistently infected, and serial infection with different FMDVs was confirmed in 13 animals. The most prevalent serotype detected was Asia-1, followed by A, and O. Phylogenetic analysis indicated multiple distinct clusters of serotypes Asia-1 and A. This study indicates a high prevalence of subclinical FMDV infection in vaccinated buffalo in Pakistan and emphasizes the importance of clinically undetected infection in FMD dynamics in endemic regions.	en_US
dc.relation.ispartof	Transboundary and Emerging Diseases	en_US
dc.relation.isbasedon	10.1111/tbed.12963	en_US
dc.subject.classification	Veterinary Sciences	en_US
dc.subject.mesh	Oropharynx	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Buffaloes	en_US
dc.subject.mesh	Foot-and-Mouth Disease Virus	en_US
dc.subject.mesh	Foot-and-Mouth Disease	en_US
dc.subject.mesh	RNA, Viral	en_US
dc.subject.mesh	Viral Vaccines	en_US
dc.subject.mesh	Vaccination	en_US
dc.subject.mesh	Longitudinal Studies	en_US
dc.subject.mesh	Prospective Studies	en_US
dc.subject.mesh	Carrier State	en_US
dc.subject.mesh	Pakistan	en_US
dc.title	Characterization of naturally occurring, new and persistent subclinical foot-and-mouth disease virus infection in vaccinated Asian buffalo in Islamabad Capital Territory, Pakistan	en_US
dc.type	Journal Article
utslib.citation.volume	6	en_US
utslib.citation.volume	65	en_US
utslib.for	0707 Veterinary Sciences	en_US
utslib.for	1117 Public Health and Health Services	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	closed_access
pubs.issue	6	en_US
pubs.publication-status	Published	en_US
pubs.volume	65	en_US

Abstract:

© 2018 Blackwell Verlag GmbH The convalescent subclinical carrier state of foot-and-mouth disease virus (FMDV) infection has been thoroughly investigated; contrastingly, the subclinical form of new infections of vaccinated and naïve hosts is recognized, but poorly understood. To investigate the natural dynamics of subclinical FMDV infections, a prospective, 12-month, longitudinal study was conducted in vaccinated Asian buffalo (Bubalus bubalis) under natural conditions in Pakistan, where FMDV is hyperendemic. Oropharyngeal fluid (OPF) samples were obtained quarterly from 300 buffalo on 30 farms which reported no clinical FMD during the 12-month study period. At the start of the study, 77.7% of buffalo had FMDV anti-NSP antibodies, and all farms had at least one seropositive buffalo. Based upon the presence of viral RNA and viral VP1 sequences obtained, distinct subcategories of subclinical infections were documented, including new, persistent, and serial infections with different FMDV strains. Viral RNA was detected in at least one OPF sample from 180 (60%) of the 300 buffalo. Over the course of the study, FMDV was detected in OPF of 80 buffalo that had been FMDV-free in previous OPF samples, indicating the occurrence of new subclinical infections. Eight buffalo were confirmed to be persistently infected, and serial infection with different FMDVs was confirmed in 13 animals. The most prevalent serotype detected was Asia-1, followed by A, and O. Phylogenetic analysis indicated multiple distinct clusters of serotypes Asia-1 and A. This study indicates a high prevalence of subclinical FMDV infection in vaccinated buffalo in Pakistan and emphasizes the importance of clinically undetected infection in FMD dynamics in endemic regions.

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http://hdl.handle.net/10453/127863