The effect of silica nanoparticulate coatings on serum protein adsorption and cellular response
- Elsevier Inc
- Publication Type:
- Journal Article
- Biomaterials, 2006, 27 (28), pp. 4856 - 4862
- Issue Date:
|dc.identifier.citation||Biomaterials, 2006, 27 (28), pp. 4856 - 4862||en_US|
|dc.description.abstract||Serum protein adsorption on colloidal silica surfaces was investigated using a quartz crystal microbalance with dissipation (QCM-D) monitoring. The amount of serum proteins adsorbed on colloidal silica-coated surfaces was not significantly different from the control silica surfaces, with the exception of 21 nm colloidal silica which experienced significantly less (P<0.05) fibrinogen adsorption compared with control silica. The adhesion and proliferation of human endothelial cells (C11STH) on nano-scale colloidal silica surfaces were significantly reduced compared with control silica surfaces, suggesting that the conformation of adsorbed proteins on the colloidal silica surfaces plays a role in modulating the amount of cell binding. Fibronectin is one of the main extracellular matrix proteins involved in endothelial cell attachment to biomaterial surfaces. There was reduced binding of a monoclonal anti-fibronectin antibody, that reacted specifically with the cell-binding fragment, to fibronectin-coated colloidal silica surfaces compared with control silica surfaces. This suggests that the fibronectin adsorbed on the colloidal silica-coated surfaces was conformationally changed compared with control silica reducing the availability of the cell-binding domain of fibronectin.||en_US|
|dc.subject.mesh||NIH 3T3 Cells||en_US|
|dc.subject.mesh||NIH 3T3 Cells||en_US|
|dc.title||The effect of silica nanoparticulate coatings on serum protein adsorption and cellular response||en_US|
|utslib.for||0903 Biomedical Engineering||en_US|
|pubs.organisational-group||/University of Technology Sydney|
|pubs.organisational-group||/University of Technology Sydney/Faculty of Science|
|pubs.organisational-group||/University of Technology Sydney/Strength - CHT - Health Technologies|
|pubs.notes||ED - OK||en_US|
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