Monitoring cell adhesion on tantalum and oxidised polystyrene using a quartz crystal microbalance with dissipation

Publisher:
Elsevier Inc
Publication Type:
Journal Article
Citation:
Biomaterials, 2006, 27 (26), pp. 4529 - 4537
Issue Date:
2006-01
Full metadata record
Files in This Item:
Filename Description Size
Thumbnail2008002380OK.pdf562.21 kB
Adobe PDF
The quartz crystal microbalance with dissipation (QCM-D) (Q-Sense AB, Sweden) has been established as a useful tool for evaluating interactions between various biological and non-biological systems, and there has been increasing interest in using the QCM-D technique for cell monitoring applications. This study investigated the potential of the QCM-D to characterise the initial adhesion and spreading of cells in contact with protein precoated biocompatible surfaces. The QCM-D technique is attractive for monitoring cell adhesion and spreading as it allows in situ real-time measurements. The adhesion of NIH3T3 (EGFP) fibroblasts to tantalum (Ta) and oxidised polystyrene (PSox) surfaces precoated with serum proteins was examined using the QCM-D for a period of either 2 or 4 h. Time-lapse photography was performed at 30 min intervals to visually examine cell adhesion and spreading in order to relate cell morphology to the QCM-D response.
Please use this identifier to cite or link to this item: