Lysozyme interaction with poly(HEMA)-based hydrogel

Lord, MS; Stenzel, MH; Simmons, A; Milthorpe, BK

Lysozyme interaction with poly(HEMA)-based hydrogel

Lord, MS Stenzel, MH Simmons, A Milthorpe, BK

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Publication Type:: Journal Article
Citation:: Biomaterials, 2006, 27 (8), pp. 1341 - 1345
Issue Date:: 2006-03-01

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Field	Value	Language
dc.contributor.author	Lord, MS	en_US
dc.contributor.author	Stenzel, MH	en_US
dc.contributor.author	Simmons, A	en_US
dc.contributor.author	Milthorpe, BK https://orcid.org/0000-0002-0867-9586	en_US
dc.date.available	2005-09-01	en_US
dc.date.issued	2006-03-01	en_US
dc.identifier.citation	Biomaterials, 2006, 27 (8), pp. 1341 - 1345	en_US
dc.identifier.issn	0142-9612	en_US
dc.identifier.uri	http://hdl.handle.net/10453/12813
dc.description.abstract	Lysozyme interaction with an acrylic-based hydrogel, poly(2-hydroxyethyl methacrylate) co-methacrylic acid (P(HEMA-MAA)), was investigated using a combination of quartz crystal microbalance with dissipation (QCM-D), surface plasmon resonance (SPR) and dual polarisation interferometry (DPI). This combination of techniques demonstrated that lysozyme initially absorbed into the hydrogel matrix and displaced water from the hydrogel while subsequent lysozyme additions were adsorbed onto the surface of the hydrogel material. QCM-D, being sensitive to bound water, showed an overall decrease in mass and stiffening of the layer after lysozyme addition. SPR, a water insensitive technique, showed a net mass increase after addition of lysozyme and buffer rinses. DPI showed that the first exposure of lysozyme to P(HEMA-MAA) was consistent with lysozyme absorption while subsequent lysozyme exposures were consistent with lysozyme adsorption. © 2005 Elsevier Ltd. All rights reserved.	en_US
dc.relation.ispartof	Biomaterials	en_US
dc.relation.isbasedon	10.1016/j.biomaterials.2005.09.007	en_US
dc.subject.classification	Biomedical Engineering	en_US
dc.subject.mesh	Polyhydroxyethyl Methacrylate	en_US
dc.subject.mesh	Polyamines	en_US
dc.subject.mesh	Muramidase	en_US
dc.subject.mesh	Hydrogels	en_US
dc.subject.mesh	Surface Plasmon Resonance	en_US
dc.subject.mesh	Interferometry	en_US
dc.subject.mesh	Temperature	en_US
dc.subject.mesh	Dose-Response Relationship, Drug	en_US
dc.title	Lysozyme interaction with poly(HEMA)-based hydrogel	en_US
dc.type	Journal Article
utslib.citation.volume	8	en_US
utslib.citation.volume	27	en_US
utslib.for	0903 Biomedical Engineering	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
utslib.copyright.status	closed_access
pubs.issue	8	en_US
pubs.publication-status	Published	en_US
pubs.volume	27	en_US

Abstract:

Lysozyme interaction with an acrylic-based hydrogel, poly(2-hydroxyethyl methacrylate) co-methacrylic acid (P(HEMA-MAA)), was investigated using a combination of quartz crystal microbalance with dissipation (QCM-D), surface plasmon resonance (SPR) and dual polarisation interferometry (DPI). This combination of techniques demonstrated that lysozyme initially absorbed into the hydrogel matrix and displaced water from the hydrogel while subsequent lysozyme additions were adsorbed onto the surface of the hydrogel material. QCM-D, being sensitive to bound water, showed an overall decrease in mass and stiffening of the layer after lysozyme addition. SPR, a water insensitive technique, showed a net mass increase after addition of lysozyme and buffer rinses. DPI showed that the first exposure of lysozyme to P(HEMA-MAA) was consistent with lysozyme absorption while subsequent lysozyme exposures were consistent with lysozyme adsorption. © 2005 Elsevier Ltd. All rights reserved.

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http://hdl.handle.net/10453/12813