Obese asthmatics are characterized by altered adipose tissue macrophage activation

Periyalil, HA; Wood, LG; Wright, TA; Karihaloo, C; Starkey, MR; Miu, AS; Baines, KJ; Hansbro, PM; Gibson, PG

Obese asthmatics are characterized by altered adipose tissue macrophage activation

Periyalil, HA Wood, LG Wright, TA Karihaloo, C Starkey, MR Miu, AS Baines, KJ Hansbro, PM

Gibson, PG

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Publication Type:: Journal Article
Citation:: Clinical and Experimental Allergy, 2018, 48 (6), pp. 641 - 649
Issue Date:: 2018-06-01

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Field	Value	Language
dc.contributor.author	Periyalil, HA	en_US
dc.contributor.author	Wood, LG	en_US
dc.contributor.author	Wright, TA	en_US
dc.contributor.author	Karihaloo, C	en_US
dc.contributor.author	Starkey, MR	en_US
dc.contributor.author	Miu, AS	en_US
dc.contributor.author	Baines, KJ	en_US
dc.contributor.author	Hansbro, PM https://orcid.org/0000-0002-4741-3035	en_US
dc.contributor.author	Gibson, PG	en_US
dc.date.available	2017-11-10	en_US
dc.date.issued	2018-06-01	en_US
dc.identifier.citation	Clinical and Experimental Allergy, 2018, 48 (6), pp. 641 - 649	en_US
dc.identifier.issn	0954-7894	en_US
dc.identifier.uri	http://hdl.handle.net/10453/128323
dc.description.abstract	© 2018 John Wiley & Sons Ltd Background: Adipose tissue-derived inflammation is linked to obesity-related comorbidities. This study aimed to quantify and immuno-phenotype adipose tissue macrophages (ATMs) from obese asthmatics and obese non-asthmatics and to examine associations between adipose tissue, systemic and airway inflammation. Methods: Visceral (VAT) adipose tissue and subcutaneous (SAT) adipose tissue were collected from obese adults undergoing bariatric surgery and processed to obtain the stromovascular fraction. Pro-inflammatory (M1) and anti-inflammatory (M2) macrophages were quantified by flow cytometry. Cytospins of induced sputum were stained for differential cell counts. Plasma C-reactive protein (CRP) and CD163 were measured by ELISA. Results: VAT contained a higher number of ATMs compared to SAT. A higher percentage of M1 ATMs was observed in VAT of obese asthmatics compared to obese non-asthmatics. The M1:M2 ratio in VAT was negatively associated with FEV1%. Sputum macrophage count was correlated positively with M1 ATMs and negatively with M2 ATMs in VAT. In obese asthmatics, CRP was positively associated with M1:M2 ratio in VAT. There were no associations with CD163. An elevated ratio of M1:M2 ATMs was observed in VAT of obese asthmatics with increased disease severity. Conclusions and Clinical Relevance: Visceral inflammation with increased pro-inflammatory macrophages (M1) occurs in obese asthma and may be a determinant of systemic inflammation and asthma severity.	en_US
dc.relation.ispartof	Clinical and Experimental Allergy	en_US
dc.relation.isbasedon	10.1111/cea.13109	en_US
dc.subject.classification	Allergy	en_US
dc.subject.mesh	Adipose Tissue	en_US
dc.subject.mesh	Macrophages	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Asthma	en_US
dc.subject.mesh	Obesity	en_US
dc.subject.mesh	Inflammation	en_US
dc.subject.mesh	Respiratory Function Tests	en_US
dc.subject.mesh	Flow Cytometry	en_US
dc.subject.mesh	Cross-Sectional Studies	en_US
dc.subject.mesh	Gene Expression Profiling	en_US
dc.subject.mesh	Macrophage Activation	en_US
dc.subject.mesh	Body Composition	en_US
dc.subject.mesh	Phenotype	en_US
dc.subject.mesh	Adult	en_US
dc.subject.mesh	Middle Aged	en_US
dc.subject.mesh	Female	en_US
dc.subject.mesh	Male	en_US
dc.subject.mesh	Biomarkers	en_US
dc.title	Obese asthmatics are characterized by altered adipose tissue macrophage activation	en_US
dc.type	Journal Article
utslib.citation.volume	6	en_US
utslib.citation.volume	48	en_US
utslib.for	1107 Immunology	en_US
utslib.for	1117 Public Health and Health Services	en_US
utslib.for	1111 Nutrition and Dietetics	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access
pubs.issue	6	en_US
pubs.publication-status	Published	en_US
pubs.volume	48	en_US

Abstract:

© 2018 John Wiley & Sons Ltd Background: Adipose tissue-derived inflammation is linked to obesity-related comorbidities. This study aimed to quantify and immuno-phenotype adipose tissue macrophages (ATMs) from obese asthmatics and obese non-asthmatics and to examine associations between adipose tissue, systemic and airway inflammation. Methods: Visceral (VAT) adipose tissue and subcutaneous (SAT) adipose tissue were collected from obese adults undergoing bariatric surgery and processed to obtain the stromovascular fraction. Pro-inflammatory (M1) and anti-inflammatory (M2) macrophages were quantified by flow cytometry. Cytospins of induced sputum were stained for differential cell counts. Plasma C-reactive protein (CRP) and CD163 were measured by ELISA. Results: VAT contained a higher number of ATMs compared to SAT. A higher percentage of M1 ATMs was observed in VAT of obese asthmatics compared to obese non-asthmatics. The M1:M2 ratio in VAT was negatively associated with FEV1%. Sputum macrophage count was correlated positively with M1 ATMs and negatively with M2 ATMs in VAT. In obese asthmatics, CRP was positively associated with M1:M2 ratio in VAT. There were no associations with CD163. An elevated ratio of M1:M2 ATMs was observed in VAT of obese asthmatics with increased disease severity. Conclusions and Clinical Relevance: Visceral inflammation with increased pro-inflammatory macrophages (M1) occurs in obese asthma and may be a determinant of systemic inflammation and asthma severity.

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http://hdl.handle.net/10453/128323